BACTERIAL
BASICS
Bacteria Structure
• Prokaryotes
– No nucleus
– Cell wall
– DNA plasmid
(ring-shaped)
• Use asexual
reproduction
http://en.wikipedia.org/wiki/Bacteria
Different Forms of DNA Transfer
Bacterial Transformation
• 1928-Frederick Griffith, 1944-Avery, McCarty, Macleod
• Process of taking up DNA from media
• DNA can be from cells
from same or different
species
• DNA will integrate into
the recipient’s
chromosome
http://en.wikipedia.org/wiki/Bacterial_transformation
Bacterial Adhesion Zones
• Adhesion zones develop in the bacteria cell
membrane, although DNA cannot fit through
Calcium Chloride Solution
• The addition of calcium ions in CaCl2 solution shields the
negatively charged phosphate groups so that the DNA can
pass through the membrane when heat shocked.
Heat Shock 42ºC