Phytochromes and Etiolation Bio 1400 Biological Inquiry e’-tee-oh-la-shun Signal Transduction • conversion of stimulus to cellular response – integrated orchestrated events • extracellular signal • causes cellular response – signal is transduced from outside of cell to inside to set up response Receptors receive stimulus from outside the cell CELL WALL CYTOPLASM stimulus chemical and electrical signals Cell response: change in gene expression DNA RNA protein Phytochrome structure in plants How do phytochromes work in plants? Phytochrome synthesis biologically ACTIVE form of phytochrome red light converts Pr to Pfr Pr continual production of Pr in plant: biologically INACTIVE form Pfr far-red light converts Pfr to Pr sunlight contains both red and far-red, thus an equilibrium is reached: 60% Pfr to 40% Pr Phytochrome synthesis biologically ACTIVE form of phytochrome red light converts Pr to Pfr Pr continual production of Pr in plant: biologically INACTIVE form Pfr far-red light converts Pfr to Pr Dark Reversion in long dark periods Electromagnetic Radiation from Sun far-red 700-800nm Absorption spectra of phytochrome forms 660nm 730nm Two parts of phytochrome: chromorphore and protein Phytochrome chromophore (Pr form) http://plantphys.info/plant_physiology/phytoch rome.shtml SIGNAL TRANSDUCTION: https://www.youtube.com/watch?v=3I2bSMm06IA&noredirect=1 Process that allows conversion of light energy chemical and electrical energy gene expression Changes in wavelength of light are detected by phytochromes http://www.bio.miami.edu/dana/226/226F08_21.html Watch the animation for more details • http://highered.mheducation.com/sites/9834 092339/student_view0/chapter41/animation_ -_phytochrome_signaling.html On your own: • Perform an internet search to investigate the function of vertebrate photoreceptors. • How are these proteins similar to photoreceptors in plants? How are they different? • Bring your findings for discussion in class on Thursday, 2-12-15