Edexcel A Level Physical Education A 9536 Edexcel Examinations A Level Physical Education A 9536 Unit 6 : Section A part 1 Scientific Principles of Exercise and Performance Previous Next Unit 6 A.1.1 INDEX Index Edexcel A Level Physical Education A 9536 3 - ENERGY - DEFINITIONS 4 - ENERGY CONCEPTS APPLICATIONS / RATES OF ENERGY EXPENDITURE 5 - INVESTIGATIONS RUNNING UP STAIRS (Margaria Staircase Test) ENERGY VALUE OF FOOD EATEN 6 - ENERGY CONCEPTS 7 - METABOLISM - ENERGY METABOLISM BASAL METABOLIC RATE (BMR) / TOTAL METABOLIC RATE 8 - MOTOR NEURONE STRUCTURE CELL BODY / DENSRITES / AXON 9 - TRANSMISSION OF NEURAL IMPULSE AN ACTION POTENTIAL 10 - TRANSMISSION OF NEURAL IMPULSE - CONDUCTION OF ACTION POTENTIAL / MYELIN SHEATH 11 - TRANSMISSION OF NEURAL IMPULSE The MOTOR END PLATE / FUNCTION / ACTION 12 - IMPULSE TRANSMISSION AT A NEUROMUSCULAR JUNCTION 13 - PRODUCTION OF COORDINATED MOVEMENT MOTOR NEURAL FIRING PATTERNS / SINGLE FIBRE TWITCH WAVE SUMMATION 14 - PRODUCTION OF COORDINATED MOVEMENT MOTOR NEURAL FIRING PATTERNS / MULTIPLE FIBRE TWITCHES / SPATIAL SUMMATION 15 - PRODUCTION OF COORDINATED MOVEMENT MOTOR NEURAL FIRING PATTERNS / MULTIPLE FIBRE TWITCHES / GRADATION OF CONTRACTION 16 - CONTROL OF COORDINATED MOVEMENT THE CEREBELLUM / THE LEARNING OF SPORTS SKILLS 17 - CONTROL OF COORDINATED MOVEMENT RECIPROCAL INNERVATION 18 - SKELETAL MUSCLE ANATOMY TENDON / CONNECTIVE TISSUE / FASCICULI / MUSCLE FIBRE / MYOFIBRIL / SARCOMERE Previous Next 19 - SKELETAL MUSCLE ANATOMY - STRUCTURE / PHYSIOLOGY 20 - SKELETAL MUSCLE ANATOMY - STRUCTURES SARCOPLASMIC RETICULUM / TROPOMYOSIN TROPONIN 21 - STRUCTURE and PHYSIOLOGY of MUSCLE CELL CONTRACTION STRUCTURE of a SARCOMERE / CONTRACTION 22 - PHYSIOLOGY of MUSCLE CELL CONTRACTION EXCITATION and CONTRACTION 23 - PHYSIOLOGY of MUSCLE CELL CONTRACTION RELAXATION 24 - STRENGTH OF MUSCLE CONTRACTION FACTORS AFFECTING 25 - ATP - ADENOSINE TRIPHOSPHATE 26 - ATP - RESYNTHESIS OF ATP 27 - ATP / PC ALACTIC SYSTEM - THE PC SYSTEM 28 - ATP / PC ALACTIC SYSTEM - THE PC SYSTEM THE COUPLED REACTION 29 - ATP / PC ALACTIC SYSTEM - THE PC SYSTEM EFFECT OF TRAINING 30 - THE LACTIC ACID SYSTEM - GLYCOLYSIS 31 - THE LACTIC ACID SYSTEM - EVENTS / SPORTS EFFECTS OF CONTINUED HIGH INTENSITY EXERCISE 32 - THE AEROBIC SYSTEM GLYCOLYSIS / KREB’S CYCLE (CITRIC ACID CYCLE) ELECTRON TRANSPORT CHAIN 33 - THE AEROBIC SYSTEM 34 - THE AEROBIC SYSTEM AEROBIC RESPIRATION / EXERCISE TYPES 35 - THE ENERGY CONTINUUM 36 - THE ENERGY CONTINUUM FACTORS AFFECTING PROPORTIONS OF ENERGY SYSTEMS VARIATION IN CONTRIBUTION OF ENERGY SYSTEMS Unit 6 A.1.2 Edexcel A Level Physical Education A 9536 Energy Concepts ENERGY DEFINITIONS ENERGY • is the capacity to do work WORK • = force x distance moved • measured in joules J CHEMICAL ENERGY • is energy that is produced by a complex series of chemical reactions • which can then be made available as : KINETIC ENERGY • is energy due to movement • which results from muscular contractions POTENTIAL ENERGY • is stored energy due to gravity POWER • is the rate at which energy is used • the energy used per second • unit watt W Previous Next Unit 6 A.1.3 Edexcel A Level Physical Education A 9536 Energy Concepts ENERGY CONCEPTS APPLICATIONS • warming-up • the construction of training programmes • prevention of fatigue • delay of fatigue • recovery from fatigue • nutrition • performance • bodyweight control • body temperature maintenance GENERAL CHARACTERISTICS • measurement of energy expenditure DIRECT METHOD • involves burning of food • and measuring heat energy produced INDIRECT METHOD • involves measurement of oxygen consumed or oxygen uptake during exercise RATES OF ENERGY EXPENDITURE (per kg of body mass) • above basal requirements kJ kg-1 min-1 sitting at rest 0.14 walking 0.2 jogging / swimming (moderate) 0.6 cycling (moderate) 0.46 vigorous exercise 0.8 Previous Next Unit 6 A.1.4 Edexcel A Level Physical Education A 9536 Energy Concepts INVESTIGATIONS RUNNING UP STAIRS (Margaria Staircase Test) • energy gained = weight x height • power = energy (joules) • time taken (sec) • answer in watts ENERGY VALUE OF FOOD EATEN • calculate full energy value of food eaten • compared with measured energy output • results show that only a small proportion of the energy is converted into useful work Previous Next Unit 6 A.1.5 Edexcel A Level Physical Education A 9536 Energy Concepts ENERGY CONCEPTS applications molecular muscle cell structures ATP definitions the recovery process tem s y ENERGY 1 ATP - PC system (alactic) s syst em 2 m te s sy lactic acid system 3 fuel for exercise sports activities and the energy continuum Previous Next aerobic system general characteristcis Unit 6 A.1.6 Edexcel A Level Physical Education A 9536 Energy Concepts METABOLISM ENERGY METABOLISM • total intake of food sufficient to supply enough energy to : – keep cells alive – keep systems working – meet demands of life BASAL METABOLIC RATE (BMR) • this is the least rate of energy usage needed to carry out basic body functions • measured after lying down after 8 hours sleep / 12 hours fasting TOTAL METABOLIC RATE • sum of BMR + energy required for all daily activities Previous Next Unit 6 A.1.7 Edexcel A Level Physical Education A 9536 Molecular Muscle Cell Structures MOTOR NEURONE STRUCTURE CELL BODY • includes nucleus and cytoplasm • membrane is receptive to stimuli from other neurones DENDRITES • highly branched processes which extend out from the cell body • specialised to receive stimuli from sensory organs or from other neurones AXON • conducts nerve impulses to other cells (nerve, muscle, gland cells) • special structures include : – myelin sheath insulates nerve – nodes of Ranvier are gaps in myelin sheath where action potential jumps from node to node – axon terminal ends with synaptic end bulbs containing neurotransmitter substances – enabling action potential to be applied to adjacent cells Previous Next Unit 6 A.1.8 Edexcel A Level Physical Education A 9536 Molecular Muscle Cell Structures TRANSMISSION of NEURAL IMPULSE AN ACTION POTENTIAL • transmission of neural messages along a neurone is an electrochemical process • an action potential is initiated when sufficient numbers of Na+ ions diffuse into the neuron • • • this depolarises the axon to a critical threshold level called the all-or-none law followed by repolarisation back to the resting potential Previous Next Unit 6 A.1.9 Edexcel A Level Physical Education A 9536 Molecular Muscle Cell Structures TRANSMISSION of NEURAL IMPULSE CONDUCTION of an ACTION POTENTIAL in a MYELINATED AXON • the myelin sheath insulates the axon • the action potential travels from node to node in a wave-like action The MYELIN SHEATH offers : • increased conduction velocity • less costly in terms of ion run-down • since ion exchange only occurs at the nodes of Ranvier Previous Next Unit 6 A.1.10 Edexcel A Level Physical Education A 9536 Molecular Muscle Cell Structures TRANSMISSION of NEURAL IMPULSE The MOTOR END PLATE FUNCTION • to transfer an impulse from the motor neurones to the muscle fibre block • this causes all muscle fibres attached to this end-plate to contract ACTION • nerve action potential is followed by the muscle action potential • a delay of 0.5 milliseconds occurs due to release of transmitter substances (such as acetylcholine from synaptic knobs) which initiate the muscle action potential • the area of depolarisation travels down muscle cell passing the entrances to ‘T’ vesicles which secrete Calcium ions needed to initiate muscle contraction • each different fibre type (slow twitch, fast twitch etc) is innervated by a separate and different type of motor neurone Previous Next The ALL OR NONE LAW • stimulation of the motor neurone will result in contraction of ALL the fibres in that motor unit SYNAPSE • a junction where the axon of one neurone interacts with another neurone Unit 6 A.1.11 Edexcel A Level Physical Education A 9536 Molecular Muscle Cell Structures IMPULSE TRANSMISSION at a NEUROMUSCULAR JUNCTION Ach = Acetylcholine Previous Next Unit 6 A.1.12 Edexcel A Level Physical Education A 9536 Molecular Muscle Cell Structures PRODUCTION OF COORDINATED MOVEMENT MOTOR NEURAL FIRING PATTERNS • all-or-none law • all fibres associated with a single motor unit are fired at once • different fibre groups (attached to different motor units) are fired at different times SINGLE FIBRE TWITCH WAVE SUMMATION • the same fibre group fired repeatedly will build the force exerted by wave summation Previous Next Unit 6 A.1.13 Edexcel A Level Physical Education A 9536 Molecular Muscle Cell Structures PRODUCTION OF COORDINATED MOVEMENT MOTOR NEURAL FIRING PATTERNS MULTIPLE FIBRE TWITCHES SPATIAL SUMMATION • different fibre groups are fired in succession to control a movement • the total force across the space of a muscle is the sum of the effect of different fibre groups Previous Next Unit 6 A.1.14 Edexcel A Level Physical Education A 9536 Molecular Muscle Cell Structures PRODUCTION OF COORDINATED MOVEMENT MOTOR NEURAL FIRING PATTERNS MULTIPLE FIBRE TWITCHES GRADATION OF CONTRACTION • refers to the ability of muscle to produce forces • varying from very light to maximum force or tension • achieved in two ways : – increasing the frequency of stimulus (wave summation) – varying the number of motor units recruited • example in hockey : – fine control of movement required for a flick – as opposed to the maximum effort required for a full hit TETANIC CONTRACTION • no time for relaxation between motor unit firing • causes complete lock up of muscle Previous Next Unit 6 A.1.15 Edexcel A Level Physical Education A 9536 Molecular Muscle Cell Structures CONTROL OF COORDINATED MOVEMENT THE CEREBELLUM • the balance of fine and gross control is under the control of the CEREBELLUM • to produce smooth coordinated movement, the cerebellum compares the intended movement with the actual movement (from sensors within the moving structure) • if a difference is detected, the cerebellum sends impulses to the appropriate motor units in the spinal cord which would produce a correction THE LEARNING OF SPORTS SKILLS • in sport, the cerebellum is involved in the learning of fine motor skills (as in archery) or gross motor skills (as in weight lifting) • gross movements use leg and arm muscles having about 1000 muscle fibres associated with one motor unit • fine movements (of the eyes and fingers for example) require muscles with far fewer (10 - 100) muscle fibres controlled by a single motor unit Previous Next Unit 6 A.1.16 Edexcel A Level Physical Education A 9536 Molecular Muscle Cell Structures CONTROL OF COORDINATED MOVEMENT CONTROL • control is achieved by changing the frequency of stimulus - wave summation • by increasing or decreasing the number of motor units in operation - gradation of contraction • and as a result of different motor units being activated in turn across a muscle - spatial summation • to enable very small forces to be maintained if required • and changes in muscle forces to occur RECIPROCAL INNERVATION • this is the self-regulation of rhythmic movements between one muscle and its antagonist • control of movements requires regulation of relaxation of antagonists during dynamic activity of an agonist Previous Next Unit 6 A.1.17 Edexcel A Level Physical Education A 9536 Molecular Muscle Cell Structures SKELETAL MUSCLE ANATOMY TENDON • dense connective tissue • attaches muscle to bone CONNECTIVE TISSUE • called fascia (epimysium, perimysium, endomysium) • holds muscle tissue together • allows free movement • stabilises nerves and blood vessels FASCICULI • bundle of muscle fibres MUSCLE FIBRE • muscle cell contains many myofibrils • several nuclei inside sarcolemma • mitochondria positioned throughout • sarcoplasm contains fat / glycogen stores, myoglobin, PC, ATP MYOFIBRIL • protein filaments SARCOMERE • the basic contractile unit Previous Next Unit 6 A.1.18 Edexcel A Level Physical Education A 9536 Molecular Muscle Cell Structures SKELETAL MUSCLE ANATOMY STRUCTURE • called STRIPED or STRIATED muscle • every muscle cell is long and thin called a MYOFIBRIL • this runs lengthways down the muscle • each muscle cell has several nuclei just under the SARCOLEMMA PHYSIOLOGY • voluntary • neurogenic (actively controlled by thoughts of sportsperson, and innervated by action of nervous system) • DEPOLARISATION (in the motor end plate) stimulates energy release • this energy provides the work necessary for muscle contraction Previous Next Unit 6 A.1.19 Edexcel A Level Physical Education A 9536 Molecular Muscle Cell Structures SKELETAL MUSCLE ANATOMY STRUCTURES INVOLVED • the motor unit • VESICLES in cell sarcoplasm pump out CALCIUM ions (Ca++) • Ca++ activates ATPase • this releases energy bond by ATP -> ADP + Pi + energy • this occurs in MITOCHONDRIA SARCOPLASMIC RETICULUM (SR) • a network of tubules and sacs in muscle cells • these t-tubules transmit muscle action potential to myofibril • the SR is also a storage site for Ca++ TROPOMYOSIN • long protein molecule that covers active myosin sites enabling actin to bind to myosin during contraction of the muscle TROPONIN • a molecule spaced at intervals along actin filaments • at rest troponin holds the tropomyosin in position to prevent exposure of the myosin binding sites on actin filaments Previous Next Unit 6 A.1.20 Edexcel A Level Physical Education A 9536 Molecular Muscle Cell Structures STRUCTURE and PHYSIOLOGY of MUSCLE CELL CONTRACTION STRUCTURE of a SARCOMERE • the Z lines are the ends of the sarcomere • which are attached to actin filaments which comprise the I zone of the relaxed unit • the myosin filaments lie in between actin filaments which they just overlap (relaxed) • the H zone is the space between the ends of the actin filaments CONTRACTION • during contraction the cross-bridges between the myosin and actin filaments pull them towards one another • this increases the overlap and pulls the Z lines towards one another Previous Next Unit 6 A.1.21 Edexcel A Level Physical Education A 9536 Molecular Muscle Cell Structures PHYSIOLOGY of MUSCLE CELL CONTRACTION EXCITATION and CONTRACTION • a neural action potential travels via a motor neurone to the motor-end plate • to create a muscle action potential over the muscle fibre’s sarcoplasm • and inward along the ‘T’ tubules • triggering the release of Ca++ from ‘T’ vesicles (located within SR) into the sarcoplasm • where it binds to a troponin molecule on an actin filament • tropomyosin molecules on the thin actin filament move exposing actin’s active sites • energised myosin cross-bridges bind to actin’s active sites • and use their energy (released from ATP) to pull the thin actin filaments towards the centre of the sarcomere (called the power stroke) • attach, detach, reattach of cross-bridges is called the ratchet mechanism • this cycle repeats itself as long as ATP is available • when fully contracted the H zone disappears Previous Next Unit 6 A.1.22 Edexcel A Level Physical Education A 9536 Molecular Muscle Cell Structures PHYSIOLOGY of MUSCLE CELL CONTRACTION RELAXATION • after the impulse is over, the SR actively pumps Ca++ back into its sacs • tropomyosin returns to its position • blocking actin’s active sites • muscle fibre returns to its resting length Previous Next Unit 6 A.1.23 Edexcel A Level Physical Education A 9536 Muscle Strength STRENGTH OF MUSCLE CONTRACTION FACTORS AFFECTING THE STRENGTH OF MUSCLE CONTRACTION metabolic condition (fatigue) amount of load (stretch reflex) STRENGTH OF MUSCLE CONTRACTION recruitment of motor units (number of fibres activated) initial length of muscle fibres (length tension relationship) Previous Next Unit 6 A.1.24 Edexcel A Level Physical Education A 9536 Energy Systems and the Energy Continuum ATP ATP - ADENOSINE TRIPHOSPHATE • • • ATP is the energy currency linked to intensity and duration of physical activity ATP exists in every living tissue its breakdown gives energy for all life functions • • • • • • energy released during tissue respiration is stored in the chemical bonds in ATP energy is released during the reaction : ATP --> ADP + Pi + energy ATPase is an enzyme which facilitates this reaction ADP is adenosine diphosphate the reaction is exothermic - it releases energy • the supply of ATP in muscle (and other tissue) will only last for 2 seconds if vigorous exercise is undertaken therefore ATP needs to be regenerated by other chemical reactions if exercise is to continue past 2 seconds • • example : an exercise like standing long jump would use energy from stored ATP Previous Next Unit 6 A.1.25 Edexcel A Level Physical Education A 9536 Energy Systems and the Energy Continuum ATP RESYNTHESIS OF ATP • • • • ATP is resynthesised from ADP within the following reaction : energy + ADP + Pi ---> ATP this is an endothermic reaction - energy is given to the molecule to enable the reaction to happen this energy will be derived from food fuels • there are 3 main systems by which this resynthesis occurs ATP - PC system (alactic) lactic acid system resynthesis of ATP from ADP, Pi and energy aerobic system Previous Next Unit 6 A.1.26 Edexcel A Level Physical Education A 9536 Energy Systems and the Energy Continuum ATP / PC ALACTIC SYSTEM - THE PHOSPHOCREATINE SYSTEM ATP / PC SYSTEM • • for activity which lasts between 3 and 10 seconds for high intensity maximum work • example : flat out sprinting - 100m sprint • no oxygen is needed - ANAEROBIC • the chemical reactions within this system are a coupled reaction Previous Next Unit 6 A.1.27 Edexcel A Level Physical Education A 9536 Energy Systems and the Energy Continuum ATP / PC ALACTIC SYSTEM - THE PHOSPHOCREATINE SYSTEM THE COUPLED REACTION • • ATP is resynthesised via phosphocreatine (PC) PC is stored in muscle cell sarcoplasm • • • • • the following reactions takes place : PC ---> Pi + C + energy energy + ADP + Pi ---> ATP the two reactions together are called a coupled reaction these reactions are facilitated by the enzyme creatine kinase • • the net effect of these two coupled reactions is : PC + ADP ---> ATP + C • PC is recreated in muscle cells during the recovery process this requires energy and is an endothermic reaction • Previous Next Unit 6 A.1.28 Edexcel A Level Physical Education A 9536 Energy Systems and the Energy Continuum ATP / PC ALACTIC SYSTEM - THE PHOSPHOCREATINE SYSTEM EFFECTS OF TRAINING ON THE ALACTIC ANAEROBIC SYSTEM • muscle cells adapt by : – increase in ATP and PC stores • therefore the ATP / PC system provides energy for slightly longer • • when exercise is taken at maximum effort the alactic / lactic threshold is delayed Previous Next Unit 6 A.1.29 Edexcel A Level Physical Education A 9536 Energy Systems and the Energy Continuum THE THE LACTIC ACID SYSTEM • depends on a chemical process called GLYCOLYSIS • glycolysis is the breakdown of sugar • • carbohydrate is stored as GLYCOGEN in the muscles and liver the breakdown of glycogen provides the energy to rebuild ATP from ADP • the breakdown of glycogen in this way is facilitated by the enzymes glycogen phosphorylase (GPP), phosphofructokinase (PFK) • this process is ANAEROBIC and takes place in the SARCOPLASM of the muscle cell no oxygen is needed • • • LACTIC ACID SYSTEM the end product of this reaction (in the absence of oxygen) is lactic acid the enzyme facilitating the conversion from pyruvic acid to lactic acid is lactate dehydrogenase (LDH) Previous Next Unit 6 A.1.30 Edexcel A Level Physical Education A 9536 Energy Systems and the Energy Continuum EFFECTS OF CONTINUED HIGH INTENSITY EXERCISE • lactic acid accumulates • this produces muscle fatigue and pain • the resultant low pH inhibits enzyme action and cross-bridge formation • hence muscle action is inhibited • physical performance deteriorates THE LACTIC ACID SYSTEM EVENTS AND SPORTS • up to 30 - 60 seconds • 400m run • 100m swim • after exercise stops, extra oxygen is taken up to remove lactic acid by changing it back into pyruvic acid • the OXYGEN DEBT TRAINING • develops body adaptations : – the ability to tolerate higher levels of lactic acid – more glycogen is stored – the lactic acid / aerobic threshold is delayed Previous Next Unit 6 A.1.31 Edexcel A Level Physical Education A 9536 Energy Systems and the Energy Continuum THE AEROBIC SYSTEM STAGE ONE - GLYCOLYSIS • this takes place in muscle cell SARCOPLASM • and is identical to the lactic acid system • ATP regenerated = 2ATP per molecule of glucose STAGE TWO - KREB’S CYCLE (CITRIC ACID CYCLE) • occurs in the presence of oxygen • taking place in the muscle cell MITOCHONDRIA within the inner fluid filled matrix • pyruvic acid (from glycolysis) promoted by enzymes of the citric acid cycle, or fatty acids (from body fat) facilitated by the enzyme lipoprotein lipase or protein (keto acids - from muscle) act as the fuel for this stage STAGE THREE - ELECTRON TRANSPORT CHAIN • occurs in the presence of oxygen • within the cristae of the muscle cell MITOCHONDRIA • hydrogen ions and electrons have potential energy which is released to produce the ATP Previous Next Unit 6 A.1.32 Edexcel A Level Physical Education A 9536 Energy Systems and the Energy Continuum THE AEROBIC SYSTEM STAGE ONE - GLYCOLYSIS STAGE TWO - KREB’S (CITRIC ACID) CYCLE • 2 molecules of pyruvic acid combine with oxaloacetic acid (4 carbons) and acetyl coA (2 carbons) • to form citric acid (6 carbons) • the cycle produces H+ and electron pairs, and CO2, and 2 ATP • fats and proteins enter the cycle STAGE THREE - THE ELCTRON TRANSPORT CHAIN • the H+ and electron pairs have potential energy • which is released in a controlled step by step manner • oxygen combines with final H+ ions to produce water and 32 ATP Previous Next Unit 6 A.1.33 Edexcel A Level Physical Education A 9536 Energy Systems and the Energy Continuum THE AEROBIC SYSTEM AEROBIC RESPIRATION • the net effect is of an endothermic reaction • glucose + 36 ADP + 36 Pi + 6O2 ---> 6CO2 + 36 ATP + 6 H2O • fat fuels produce 2 ATP less than glucose AEROBIC EXERCISE TYPES • low intensity, long duration activities • examples : game of hockey, 800m swim, long distance jog Previous Next Unit 6 A.1.34 Edexcel A Level Physical Education A 9536 Energy Systems and the Energy Continuum THE ENERGY CONTINUUM THE ENERGY CONTINUUM • this describes the process by which ATP is regenerated via the different energy systems • depending on the intensity and duration of exercise • • • each of the alactic, lactic acid and aerobic systems contribute some ATP during the performance of all sports one or other of the energy systems usually provides the major contribution for a given activity the diagram shows approximate proportions of ATP resynthesised via aerobic / anaerobic for some sporting activities Previous Next Unit 6 A.1.35 Edexcel A Level Physical Education A 9536 Energy Systems and the Energy Continuum THE ENERGY CONTINUUM OTHER FACTORS AFFECTING THE PROPORTIONS OF ENERGY SYSTEMS • used in any given exercise activity are : – level of fitness (whether adaptations to training have included enhancement of relevant enzymes - which would for example postpone levels of lactate accumulation) – availability of O2 and food fuels, for example a high CHO diet would assist replenishment of glycogen stores which would then be available for glycolysis VARIATION IN CONTRIBUTION OF ENERGY SYSTEMS • as time progresses during intense exercise, the following chart shows the contribution of the different energy systems to the resynthesis of ATP Previous Next Unit 6 A.1.36