30.10.2014 Chromosomal Anomalies (Lecture 2) Dr. Archana Rani Associate Professor Department of Anatomy KGMU UP, Lucknow Trisomy of Sex Chromosomes • Klinefelter syndrome • Triple X syndrome • Double Ysyndrome Klinefelter Syndrome • Chromosome complement: 47,XXY • Phenotype: Male • Incidence: 1:1000 Features of Klinefelter Syndrome • Tall stature; thin build; long lower limbs • Testicular atrophy • Female pattern of pubic hair • High pitched voice • Infertility (aspermatogenesis) • Gynaecomastia • Low level of intelligence • Serum testosterone levels low to normal • FSH and LH levels very high • Sex chromatin positive Klinefelter syndrome: Karyotype Klinefelter Syndrome Triple X Syndrome (Superfemale) • Chromosome complement: 47,XXX • Phenotype: Female • Incidence: 1:1000 Features of Triple X Syndrome • Normal in appearance • Difficulty in speech, learning and emotional responses • Mild mental retardation in 15-25% cases • Two sex chromatin Barr bodies • Infertility • • • • • Wide-set eyes Amenorrhoea Expressionless face Enamel hypoplasia Deficient language skills • Delayed development of motor skills Superfemale: Karyotype Superfemale Double Y Syndrome • Chromosome complement: 47,XYY • Phenotype: Male • Incidence: 1:1000 Features of Double Y Syndrome • Normal in appearance • Tall stature • Aggressive behaviour • Problems in development motor and language Monosomies of Chromosomes • Presence of only one member of a chromosome pair in a karyotype • More detrimental than equivalent trisomy • Can involve autosomes or sex chromosomes • Usually abort spontaneously • Monosomy of X chromosome results in XO condition called Turner syndrome Turner Syndrome • Chromosome complement: 45,XO • Phenotype: Female • Incidence: 1:5000-8000 Features of Turner Syndrome • Short statured female • Sexual infantilism with primary amenorrhoea and sterility • Short, webbed neck • Prominent ears with defective hearing • Small mandible • Defective vision Features of Turner Syndrome • • • • • • • • Epicanthal folds Low posterior hair line Cubitus valgus Broad chest with widely spaced nipples Cardiovascular anomalies Hyperconvex finger nails Pigmented nevi Sex chromatin negative Turner Syndrome: Karyotype Turner Syndrome Structural Chromosomal Abnormalities • Results from chromosome breakage • Followed by reconstitution in an abnormal combination • Breaks in any chromosome may be induced by various factors Structural Chromosomal Abnormalities • Deletion (Deficiency) • Inversion • Translocation • Isochromosome • Ring Chromosome Deletion • Loss of a (generally small) segment of chromosome A B C D E F G A B D E F G C Deletion • Arise through spontaneous breakage – some chromosomes have fragile spots – radiation, UV, chemicals, viruses may increase breakage Deletion • May arise through unequal crossing over A B C D E F G x A B A B C D E G Deletion C D A B E F G C D E F F G Duplication Deletions in Humans • Cri-du-chat syndrome – Micro deletion of chromosome 5 • Di-George syndrome – Micro deletion of chromosome 22 • Schizophrenia & Obsessive Compulsive Disorder – Micro deletion of chromosome 22 associated • Angelman syndrome – Micro deletion of chromosome 15 • Prader-Willi syndrome – Micro deletion of chromosome 15 Cri-du-chat syndrome • • • • • • • • • • 1st autosomal deletion described Characteristic cat-like cry, which disappears with age Microcephaly Severe mental retardation Congenital heart disease Hypertelorism (widely separated eyes) Low birth weight and poor growth Severe cognitive, speech, and motor delay Behavioral problems Excessive drooling Cri-du-chat syndrome Prader-Willi and Angelman Syndromes Prader-Willi Syndrome • Lack of muscle tone in newborn • Poor swallowing reflex • As adult - gross obesity • Mean I.Q. ~ 50 • Microdeletion of 15 Angelman Syndrome • Developmentally delayed • Jerky movements • Stiff, fixed smile • Uncontrolled laughter • Abnormal E.E.G., epilepsy • Microdeletion of 15 Inversion • 180o reversal of chromosome segment A B C D E F G H I J K D I J K 180O A B C H G F E Inversion • Produced through breakage and reassociation of chromosome D E C A B F G Inversion • Produced through breakage and reassociation of chromosome D E C A B F G Types of Inversion Paracentric Pericentric Translocation • Exchange of segments between nonF homologous chromosomes E L M D N O C B A P Q Translocation A B C Q O P N D M E L F Isochromosome • Centromere of the chromosome divides transversely instead of longitudinally • One arm is missing and the other arm duplicated Ring Chromosome • Occurs due to loss of both the ends of a chromosome • The broken ends rejoin to form a chromosome • Rare anomaly ring-like Robertsonian Changes • Fusion two chromosomes join to form one • Fission one chromosome splits to form two REFERENCES 1. Essentials of Anatomy for Dentistry Students,1st Edition. 2. Langman’s Medical Embryology,11th Edition. 3. Human Embryology, 5th Edition. MCQs 1. Klinefelter syndrome is associated with chromosome complement: a) 47,XXX b) 47,XXY c) 47,XYY d) 47,YYY MCQs 2. Testicular atrophy is associated with: a) Triple X syndrome b) Double Y syndrome c) Turner syndrome d) Klinefelter syndrome MCQs 3. Sex chromatin negative is a characteristic feature of: a) Triple X syndrome b) Down syndrome c) Turner syndrome d) Klinefelter syndrome MCQs 4. All of the following are trisomy of sex chromosomes except: a) Turner syndrome b) Klinefelter syndrome c) Triple X syndrome d) Double Y syndrome MCQs 5. Partial deletion of short arm of chromosome 5 is a feature of: a) Angelman syndrome b) Prader -Willi syndrome c) Cri-du-chat syndrome d) All of the above