How to excel in
transferring
Qijun (Daniel) Hu
B.S. Bioengineering, 2015
UC Berkeley
A.S. Chemistry, Mathematics, 2013
Chabot College
Outline
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About me
Berkeley Bioengineering
Internships (UCB, ACPWA, JBEI, DuPont)
How to get internships
Transfer Tips
After transfer
11/11/2014 | Daniel Hu
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A little about me
• Came to US, 2011
• Chabot/LPC, 2011-2013
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Tutor and mentor in MESA
TA for Mrs. Chaudhuri
Alpha Gamma Sigma Honor society (AGS)
Mu Alpha Theta Honor Society (math club in LPC)
• UC Berkeley, 2013-2015
– Tau Beta Pi Engineering Honor Society (TBP)
– Bioengineering Honor Society (BioEHS)
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Berkeley Bioengineering1
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Biomaterials
Biomechanics
Cell and Tissue engineering
Biomedical Devices
Computational Biology
Premed
Synthetic Biology
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Biomaterials, Biomechanics, and
Cell & Tissue Engineering
http://galleryhip.com/biomechanical-engineering-prosthetics.html
http://www.uweb.engr.washington.edu/research/tut
orials/introbiomat.html
http://www.chem-eng.kyushu-u.ac.jp/lab3/researches_eng.html
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Synthetic Biology
http://brandonbid.wix.com/syntheticmicrobes
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Outline
•
•
•
•
•
•
About me
Berkeley Bioengineering
Internships (UCB, ACPWA, JBEI, DuPont)
How to get internships
Transfer Tips
After transfer
11/11/2014 | Daniel Hu
7
Transfer-to-Excellence (TTE)
• Lea Marlor, Nov-25-14, Engr 10 lecture
• Hands on research experience
• http://www.e3s-center.org/education/edu-tte-reuappl2.htm
• Additional advising and support for transfer to a 4year institution during academic year with TAP
Program
• http://cep.berkeley.edu/tap
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y
s
e
e
e
el
TTE - The Duber
OH
HO2C
OH
O
HO2C
OH
Lab2
b
HO2C
GBD
OH
HO2C
SCoA
O
OH
O
O
OUTPU
SCoA
HO2C
SCoA
SCoA
SH3
PDZ
complex shows a largely hydrophobic, protected tunnel that connects
• active
Enzymes
chemical
sites about 25catalyst
Å apart14. In addition
to protectionreactions
of reactive
Modular machinery
intermediates,
this
tunnel
actively
passes
the
indole
intermediate
domains/motifs)
• Synthetic protein scaffolds is an efficient way(interaction
to coa
between active sites, thereby increasing reaction kinetics. Similarly, the
Metazoan cell
E. coli cell
localize
the
enzymes
crystal structure of carbomoyl phosphate synthetase shows a tunnel
15. Bicarbonate, glutamine
Scaffolding:
96 Å long,Mevalonate
which connects
three
active
sites
pathway
E. colithrough
cell
and
a seriesof four sepaYeast
celltwo molecules of ATP are channeled
Enzymerate
pipeline:
reactions that produce three reactive and unstable intermediates:
NH 3, carboxyphosphate and carbamate12. Channeling couples these
INPUT
required reactions at the proper stoichiometry despite a three-orderAtoB
of-magnitude
difference inHMGS
the Km for free NH 3 compared to that c Input
for glutamine. Polyketide synthases offer another interesting case of
OUTPUT
substrate channeling—modulescomprising assembly linesof catalytic
Output
activitieswheresubstratesaredirectly tethered to, and processively carb
ried through, theenzymemodulesviathioester linkages. In thismanner,
complex small moleculescan besynthesized with very low ratesof loss artemisinin. As the mevalonate biosynthetic pathway is not en
of intermediates.
These
natural
genous to Escherichia coli, its heterologous expression can resul
Modular
machiner
y examples illustrate two approaches of
(interaction domains/motifs)
substrate
channeling: sequestration versusE.covalent
tethering of inter- flux imbalances with high metabolic load in this production ho
Metazoan cell
coli cell
9
11/11/2014
Hu acetoacetyl-C
mediates. Instead of undertaking the difficult task of engineering pro- Whereas the first enzyme in the
synthetic| Daniel
pathway,
009 Nature America, Inc. All rights reserved.
y
Thus, both the mevalonate pathway enzymes and the regulation machinery
are heterologous to E. coli. (c) The scaffolded pathway is more efficient
as a result of co-localizing the mevalonate enzymes to the same complex
as well as optimizing the enzyme stoichiometry to balance the units of
activity at the complex.
AtoB
HMGS
O
HMGR
O
O
O
O
SCoA
O
O
SCoA
SCoA
SCoA
SCoA
HMGR
OH
HO2C
OH
O
HO2C
OH
HO2C
GBD
Scaffolding:
SH3
PDZ
OH
HO2C
SCoA
O
OH
O
O
SCoA
HO2C
SCoA
SCoA
OH
A RTI CLES
Design Synthetic Protein
Scaffolds
aP
b
PBAD
TET
AtoB
HMGS
HMGR
SCAFFOLD
GBD
GBD
N
HMGS
HMGR
x
SH3
SH3
AtoB
y
PDZ
• Small peptides that can binds
to protein scaffolds
• Change ratio between
domains (x:y:z)
C
z
PDZ
x, y, z = number of
domain repeats
Figure 3 Synthetic scaffolds built from modul
mevalonate pathway was placed under express
scaffolds were constructed with three proteinthese domains, respectively. (b) A matrix of ni
10
11/11/2014SH3
| Daniel Hu
of recruitment domains (GBD
1
2 PDZ2 or G
Production in mevalonate acid
HMGR
SCAFFOLD
GBD
GBD
N
HMGR
SH3
SH3
HMGS
x
y
PDZ
AtoB
C
z
x, y, z = number of
domain repeats
PDZ
Mevalonate production
(normalized to maximum
HMGS
c
N
80
70
60
50
40
30
20
10
0
x=1
4
2
1
1
2
4
z (no. PDZ domains)
HMGR
C
y (no. SH3 domains)
HMGS
SH3
AtoB
b
GBD
BAD
PDZ
TET
Fold increase mevalonate
product over no scaffold
ARTI CLES
• Best ratio x:y:z = 1:2:2
• Increased 77-fold in
aP
P
production titer compare to
no scaffold
y
z
Figure 3 Synthetic scaffolds built from modular protein-protein interaction domains provide modular co
mevalonate pathway was placed under expression control of PTET, and expression of the synthetic scaffo
scaffolds were constructed with three protein-protein interaction domains (GBD, SH3 and PDZ), where
these domains, respectively. (b) A matrix of nine scaffolds exhibited dramatic differences in mevalonate
of recruitment domains (GBD1 SH3 2 PDZ2 or G1 S2 P2 ) for maximum pathway flux resulted in a 77-fold in
scaffolded pathway (G0 S0 P0 ). Improvements in mevalonate production levels were strongly dependent o
11
Daniel Hu
Table 8). (c) The scaffold induction level for optimal mevalonate production 11/11/2014
depends| on
the number
of
Alameda County Public Works
Agency
• Alameda Creek Watershed Steelhead Restoration
• Bringing salmon and steelhead trout back to Alameda
Creek.
http://bayarearides.com/rides/alamedacreek/
http://upload.wikimedia.org/wikipedia/commons/1/1d
/Female_Rainbow_Trout_in_hand.JPG
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Watershed
http://water.epa.gov/type/watersheds/whatis.cfm
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Watershed
http://www.alamedacreek.org/learn-more/map-watershed.php
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Flood Channel
• Rain fall data from
watershed
• The 100-year storm
control requirement
(flood event that has 1%
http://baytrail.abag.ca.gov/vtour/map4/access/AlamedaNW/AlamedaNW.htm
probability of
occurring in any given
year)
http://www.nzdl.org/gsdlmod
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AutoCAD Civil 3D
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AutoCAD Civil 3D
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Joint BioEnergy Institute
• Develop biofuels from cellulosic material
http://www.gopixpic.com/640/img-00044jpg/
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The mevalonate pathway
• Biofuels:
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Chromosomal Integration into E.
Coli
• More stable
• No use of antibiotics
• Consistent expression
TOP
CmR
IPP
BIS
FRT
MEV
FRT
GLC
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DuPont-Industrial Biosciences
• 13-week summer internship program
• Investigating cell component dynamics in Trichoderma
reesei
• Cellulolytic enzymes
http://www.fungalcell.org/hyphal-tip-growth-and-branching-image-gallery
http://www.juanablog.com/tutoriales/trichoderma
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Best succeed in an internship
• Be detail – bring a notebook and a pen everywhere you
go
• Networking – talk to as many people in the company as
possible
• Ask for extra work if you have extra time.
• Ask for reference at the end of the internship
• Keep the connection going
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Outline
•
•
•
•
•
•
About me
Berkeley Bioengineering
Internships (UCB, ACPWA, JBEI, DuPont)
How to get internships
Transfer Tips
After transfer
11/11/2014 | Daniel Hu
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Why internship?
• Internship is the king
• Universities will more likely to accept students with
internship experience
• Discover what you love or hate to do
• Working experience is the most important thing for you to
find a job after graduation
• However, GPA is the most important for engineering
transfer!
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Please be very serious about your
resume
• Frist thing that employers look
at
• Be careful what to put on the
first half page
• Order everything by relevance
of the job that you apply
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Learn the job description
• In your resume –
write your skills
exactly matching the
words in the
application
• Briefly learn
everything they
mention in the
application
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Be prepared to answer nontechnical questions
• Why you want to work for our company?
• Is there anything else you want to tell us?
• After interview, ALWAYS!!
– Send a thank you email.
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Outline
•
•
•
•
•
•
About me
Berkeley Bioengineering
Internships (UCB, ACPWA, JBEI, DuPont)
How to get internships
Transfer Tips
After transfer
11/11/2014 | Daniel Hu
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Do not always trust assist.org
• Why does EECS major
need a bunch of bio, chem
classes??
• Be curious, always ask
why!
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This is the correct way
• Straight from EECS
transfer website in
Berkeley
• Have to finish all the
“Must complete” on the
university transfer
website
• Best to confirm with the
university counselor
http://www.eecs.berkeley.edu/Programs/ugrad/transfer-reqs.shtml
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Other transfer tips
• Try to take some classes outside of your major
• Apply for as many scholarships as you can while you are
in CC
• Don’t spend extra time to get A.A. or A.S. degree
• Things that I regret not doing in Chabot
– Take Engr 22, 43, 45, and microbiology classes
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Outline
•
•
•
•
•
•
About me
Berkeley Bioengineering
Internships (UCB, ACPWA, JBEI, DuPont)
How to get internships
Transfer Tips
After transfer
11/11/2014 | Daniel Hu
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After transfer
• Don’t take too many classes in your first semester
• Join some clubs
• Try to get very high GPA
– This is the GPA that summer intern employers look at
• As always! Find internship opportunities
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SAGE (Student Achievement
Guided by Experience)
• Professional development class in Haas School of
Business
• Networking
• Coaching
• Resume, cover letter
• Pay internship
• http://sagescholars.berkeley.edu
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Final advice
• Know your professors in Chabot College, they are very
nice and willing to help you much more than most of the
university professors!
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Questions?
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Thank you!
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Reference
1.
2.
http://bioeng.berkeley.edu/undergrad/program/concentrations
Dueber JE, Wu GC, Malmirchegini GR, Moon TS, Petzold CJ, Ullal A
V, et al. Synthetic protein scaffolds provide modular control over
metabolic flux. Nat Biotechnol [Internet]. 2009 Aug [cited 2014 Jul
14];27(8):753–9. Available from:
http://www.ncbi.nlm.nih.gov/pubmed/19648908
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