Preparing for a lab - Biology Learning Center

advertisement
1
Welcome to
Bio181L!
Contemplating the nuts & bolts of Life
xVivo’s 3D cell animation
(also on TA desktop--Lab01TaoTA => Biovisions => BioVisionsPlayer.swf)
About me...
•
Kelsey Berg
•
kberg@email.arizona.edu
•
Majors in Physiology/Molecular and Cellular Biology,
Minor in Chemistry
•
From Phoenix, Arizona
2
What 181L is
•
Thinking, Understanding, Investigating, Evaluating
•
•
Not arrive, assembly line, leave
Not synchronized with most lecture sections, but internally
coherent & mutually reinforcing
3
Science and this course
•
“One of the beautiful things about science is that it allows us to
bumble along, getting it wrong time after time, and feeling
perfectly fine as long as we learn something each time.”
4
5
About you
Last name, First name
Section #
Year
Major(s)
Minor(s)
What do you want to do (career)?
Where are you from?
What do you expect from this course?
Anything else that I should know about you (optional)?
•
NOTE: The section dance is now
over
•
Once a lab has met, no one can
change into it
About the manual
•
Reading it will prepare you for class & in-class quizzes
•
Reading prior to 5’ before class will allow you to understand
& think about what you’re reading
•
Lays out Philosophy (vii), Learning Goals (ix), expectations (xi)
•
Contains Periodic Table (0-1) and Molecular Basics (0-3), rules
of molecule pics (0-5) and a cell (0-7)
6
181 Lab ACCOUNTS
•
http://blc.arizona.edu/courses/181lab
•
Click ‘Create Accounts’
•
Your section number is:
•
Bio181L_Go is the best way to do your work. Browser not so
much
XX
7
80% of success is just showing up*
•
Absences must be excused from from Asya Roberts in BSE109
prior to making up
•
Arriving late is absent. Leaving early is absent.
•
Labs cannot be offered week after they are delivered; Avoid
missing a lab
•
Missing 2 labs blows a huge hole in the intellectual content.
You may be dropped from the course--excused or otherwise
*--Woody Allen
8
My Webpage
•
Get there via homepage* => Instructors => (Section #)
*http://blc.arizona.edu/courses/181Lab
(Lab Man yellow page)
9
Web page operation
10
What to expect
•
Homework is serious--start early, and work in bursts, with gaps.
Generally, 2-3 components/week
•
You will play a major role in experiment, protocol design
•
i.e., you’ll need to know what you’re trying to achieve rather
than showing up & following steps. Sometimes, there aren’t
any
11
Policies & Grading
•
Syllabus (Linked on course homepage)
•
•
Honor code & Plagiarism (Manual, p. xiii)
Assignments
•
On-line assessments/tutorials (10%)
•
In-class quizzes (15%)
•
Lab assignments (LABAs--Lab Activity Based Assignments)
(50%)
•
Lab Projects & reports (combine to 25%)
12
13
How?
Why?
the Tao of Molecules
How molecules feel & the world they live in
Primary goals
• Create understanding by observation,
•
reasoning
Chemical foundations for the course:
• Water & its properties
• non-watery things
• Know molecules as real & tangible things
14
15
When a journalist asked the great physicist Richard
Feynman what single sentence would best
encapsulate all science so far if it were to be the
sole surviving scrap of all we knew, he replied, “The
world is made of atoms.”
From The Secret of Scent, Luca Turin p.28
16
Atoms: They’re how life works
•
DNA, RNA: C, H, N, O, P, [Mg++]
•
Carbs: C, H, O
•
Protein: C, H, N, O, (S), (P), [traces]
•
Membranes: C, H, N, O, P, (S)
What cannot be done with assemblages of these atoms*
cannot be done by living organisms, nor their cells, nor their
spit, etc.
*OK, fine, there’s the occasional role for Ca++, etc.
Who am I?
17
•
At birth, # protons = # electrons
•
Atoms seek completion, which means outermost electron set = 8
(hydrogen, helium it’s just 2)
Freeman Fig. 2.1a
Coloring your world
18
Four views of Water
(There will be a test)
+
H2O
+
-
H
See lab manual, p. 0-3
O
H
19
Interacting with H2O
To your StructViewers!
Desktop => Bio181L_Go
20
21
Doing it
Clean up!
•
Oil waste in the hood
•
ethanol waste in the sink
•
anything too messy wadded up and discarded
22
“Salting out”
•
It’s a real term; used to refer to a situation where the addition of
salt (portable charges) alters the solubility of other molecules in
water
•
Why should this be?
23
Evaporation
24
•
First things first--what is it? What’s going on in terms of molecules
•
Experiment: Ethanol and water on your arm: which is cooler & why?
•
Prediction: based on molecular weight, which should evaporate
more quickly--H2O (2 x 1 + 1 x 16) or CH3CH2OH (5 x 1; 2 x 12; 1 x 16)
•
If not, why not?
•
Salt--ever tasted your sweat? Or anybody else’s for that matter?
•
What benefit might there be to adding NaCl to water that you
are intending to evaporate?
Some advice
25
http://www.damnlol.com/please-do-me-right-now-224.html
What’s an Assessor?
=>Chatting<=
26
What’s vocabulary homework?
•
EITHER both versions of the crossword exercise
•
OR VocabuWary with a better than threshold score
•
For “Atoms & Molecules”, F12, that’s 60,000
•
Errors count off. Slowness counts off.
27
Homework
due 10 p.m. before lab
Assessor: 181 Intro ’12
Vocab Atoms/Mols: xWord(x2) or ‘Wary
Assessor: Molecular World Tutorial
Next week’s quiz will include
Concepts from today
Atom colors
Deducing partial charges (from tutorial)
Manual Ch. 2
28
Download