Preparing for a lab

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Welcome to
Bio181L!
Contemplating the nuts & bolts of Life
“It’s atoms all the way down”
About me...
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About you
1.
Name
2.
Where you are from
3.
Major and year (freshman, sophomore, etc.)
4.
What you do in your free time
5.
What you want to do when you graduate
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What you tell your relatives (may be the same)
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What 181L is
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Thinking, Understanding, Investigating, Evaluating
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Not arrive, assembly line, leave
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Manual pp. ix & xi should help
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Not sync’ed with most lecture sections, but internally coherent &
mutually reinforcing
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Expectations
• Read the lab manual BEFORE lab
• Be ON TIME to class
• PARTICIPATE
• Complete the assignments (online, etc)
• Participate
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About the manual
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Reading it will prepare you for class & in-class
quizzes
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Reading prior to 5’ before class will allow you to
understand & think about what you’re reading
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Lays out Philosophy (vii), Learning Goals (ix),
expectations (xi)
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Contains Periodic Table (0-1) and Molecular
Basics (0-3), rules of molecule pics (0-5) and a cell
(0-7)
181 Lab ACCOUNTS
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http://blc.arizona.edu/courses/181lab (Safari home page)
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Click ‘Create Accounts’
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Your section number is:
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Bio181L_Go is the way to do your work. Browser bad
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Where to find 181L_Go and a movie explaining how to download it to your computer
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80% of success is just showing up*
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Absences must be excused by Asya Roberts in BSE109 prior to
making up. Contact her as soon as possible. Really.
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Late is absent. Leaving early is absent.
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Labs cannot be offered week after they are delivered; Avoid
missing a lab
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Missing 2 labs => you’ll be dropped from the course--excused
or otherwise (b/c you’ll have little shot at learning what you
need)
*--Woody Allen
My Webpage
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Get there via homepage* => Instructors => Sxn#
*http://blc.arizona.edu/courses/181Lab
(Lab Man yellow page)
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Web page operation
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Policies & Grading
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Syllabus (Linked on course homepage)
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Honor code & Plagiarism (Manual, p. xiii)
Assignments
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On-line assessments/tutorials (10%)
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In-class quizzes (15%)
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Lab assignments (LABAs--Lab Activity Based Assignments) (50%)
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Lab Projects & reports (combine to 25%)
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Every assignment is posted & reported on basis of 100%
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For electronic assignments, log in again to see your recorded score
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Also reported to you at the end
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How?
Why?
the Tao of Molecules
How molecules feel & the world they live in
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Primary goals
• Creating understanding by observation and
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reasoning
Establish the chemical foundations for the
course:
• Water & its properties
• non-watery things
Start thinking of molecules as real & tangible
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What do you know
about oil and water?
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What do you know
about oil and water?
• Today is all about understanding what you
“know”
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When a journalist asked the great physicist Richard
Feynman what single sentence would best
encapsulate all science so far if it were to be the
sole surviving scrap of all we knew, he replied,
“The world is made of atoms.”
From The Secret of Scent, Luca Turin p.28
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Atoms: They’re how life works
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DNA, RNA: C, H, N, O, P, [Mg++]
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Carbs: C, H, O
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Protein: C, H, N, O, (S), (P), [traces]
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Membranes: C, H, N, O, P, (S)
What cannot be done with assemblages of these atoms*
cannot be done by living organisms, nor their cells, nor their
spit, etc.
*OK, fine, there’s the occasional role for Ca++, etc.
Who am I?
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At birth, # protons = # electrons
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Atoms seek completion, which means outermost electron set = 8
(hydrogen, helium it’s just 2)
Freeman Fig. 2.1a
Dependent on:
# protons (+ve charge)
Distance e- from nucleus
Slide courtesy of Cara Gibson
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Symbolizing
Atoms & Molecules
Coloring your world
**memorize these – they will be on your quiz next week
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Views of Water
(There will be a test)
+
H2O
+
-
H
See lab manual, p. 0-3
O
H
More on colors
• RED also means
‘negative’
• Blue also means
‘positive’
Interacting with H2O
To your StructViewers!
Desktop => Bio181L_Go
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Doing it
Computers, stations
Write your group name down; otherwise you
can’t retrieve your work!
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Group names: Just use the first three letters
of everyone’s name
i.e., emikevbru
Write this down so you can look at your work
latter if you want to
You will all just go until you get to the stuff
about alcohols and salt and then we will
talk again
How to draw molecules
Carbon Hydrogen Oxygen Nitrogen
Figure is from Lab Manual p. 0-5
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TA-lead discussions
“Salting out”
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It’s a real term; used to refer to a situation where the addition of
salt (portable charges) alters the solubility of other molecules in
water
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Why should this be?
Evaporation
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First things first--what is it? What’s going on in terms of molecules
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Experiment: Ethanol and water on your arm: which is cooler & why?
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Prediction: based on molecular weight, which should evaporate
more quickly--H2O (2 x 1 + 1 x 16) or CH3CH2OH (5 x 1; 2 x 12; 1 x 16)
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If not, why not?
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Salt--ever tasted your sweat? Or anybody else’s for that matter?
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What benefit might there be to adding NaCl to water that you
are intending to evaporate?
Clean up!
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Oil waste in the hood
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ethanol waste in the sink
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anything too messy wadded up and discarded
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Some advice
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http://www.damnlol.com/please-do-me-right-now-224.html
What’s an Assessor?
=>Chatting<=
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What’s vocabulary homework?
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EITHER both versions of the crossword exercise
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OR VocabuWary with a better than threshold score
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For “Atoms & Molecules”, F12, that’s 60,000
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Errors count off. Slowness counts off.
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Homework
due 10 p.m. before lab
Assessor: 181 Intro ’12
Vocab Atoms/Mols: xWord(x2) or ‘Wary
Assessor: Molecular World Tutorial
Next week’s quiz will include
Concepts from today
Atom colors
Deducing partial charges (from tutorial)
Manual Ch. 2
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