AP Biology Korzeniewski Name_______________________________ Date___________________Per_________ Metabolism Notes #5 – Photosynthesis Objectives: Be able to explain the process of photosynthesis. Observe the relationship between photosynthesis and cellular respiration. Key Vocabulary Heterotrophs Autotrophs Thylakoid Membrane Photosystem I Stomates Chlorophyll Photosystem II Chloroplast Light Reactions Chlorophyll a Stroma Calvin Cycle Chlorophyll b Thylakoid Sacs Grana stacks NADPH RuBisCo G3P C3 Photosynthesis Photosynthesis Part 1– Life from Light and Air Energy needs of life All life needs a constant input of energy o _________________________________ get their energy from “eating others” eat food = other organisms = ___________________ make energy through ____________________ o ___________________________________ produce their own energy (from “self”) convert energy of sunlight build ____________________________________ make energy & synthesize sugars through __________________ How are they connected? Write the two reactions and the relationship between them: What does it mean to be a plant Need to… o ___________ light energy transform it into chemical energy o __________ light energy in a stable form to be moved around the plant or stored o need to get ______________________________________ _____________________________ o produce all ___________________ needed for growth carbohydrates, proteins, lipids, nucleic acids Plant structure o Obtaining raw materials o _____________ ______________ = solar collectors o _______________ ________________= gas exchange o _________________ uptake from ________________ o ___________________ N, P, K, S, Mg, Fe… uptake from ______________ Plant structure Chloroplasts o double membrane o stroma fluid-filled interior o thylakoid sacs o grana stacks Thylakoid membrane contains o chlorophyll molecules o electron transport chain o ATP synthase H+ gradient built up within thylakoid sac Photosynthesis o o Light reactions o light-dependent reactions o _________________________________________________ convert solar energy to chemical energy ATP & NADPH Calvin cycle o light-independent reactions o ________________________________________________________ uses chemical energy (ATP & NADPH) to reduce CO2 & synthesize C6H12O6 Light reactions o o o _________________________________________________ o like in cellular respiration proteins in organelle membrane ___________________________________________ o ___________________________________ o proton (H+) gradient across inner membrane find the double membrane! o ATP synthase enzyme ETC of Respiration: Mitochondria transfer ___________________________________________________ o Use electron carrier _________________ ETC of Photosynthesis: Chloroplasts transform __________________________________________________ o Use electron carrier _________________ The ATP that “Jack” built … Copy this slide: Pigments of photosynthesis Chlorophylls & other pigments o embedded in thylakoid membrane o arranged in a “photosystem” collection of molecules o structure-function relationship A Look at Light The spectrum of color o Shorter wavelength ------------------------____________________ o Higher energy ------------------------_____________________ Light: absorption spectra Photosynthesis gets energy by absorbing wavelengths of light o chlorophyll a absorbs best in red & blue wavelengths & least in green o accessory pigments with different structures absorb light of different wavelengths chlorophyll b, carotenoids, xanthophylls Photosystems of photosynthesis o 2 photosystems in thylakoid membrane o collections of chlorophyll molecules o act as light-gathering molecules o Photosystem II _____________________________ P680 = absorbs 680nm wavelength red light o Photosystem I ______________________________________ P700 = absorbs 700nm wavelength red light ETC of Photosynthesis ETC uses __________________________ to produce o ______________________________________ go to Calvin cycle PS II absorbs light o excited electron passes from chlorophyll to “primary electron acceptor” o need to replace electron in chlorophyll o enzyme ______________________________________ supplies them to chlorophyll ___________________________________ combines with another O to form O2 _____________________________________________ and we breathe easier! Photosynthesis summary Where did the energy come from?_____________________ Where did the electrons come from? _______________________ Where did the H2O come from? _________________________ Where did the O2 come from? __________________________ Where did the O2 go? ___________________________ Where did the H+ come from? _______________________ Where did the ATP come from? ________________________ What will the ATP be used for? __________________________ Where did the NADPH come from? ____________________________ What will the NADPH be used for? _______________________________ Photosynthesis Part 2: The Calvin Cycle ---- Life from Air Remember what it means to be a plant… Need to _______________________ necessary for growth o carbohydrates, lipids, proteins, nucleic acids Need to store ____________________________________________ o in a more stable form o that can be moved around plant o saved for a rainy day Light reactions o o Convert solar energy to chemical energy o ATP ______________ o NADPH _____________________ What can we do now? _______________________________________ How is that helpful? o Want to make C6H12O6 o synthesis o How? From what? What raw materials are available? Carbon Fixation: Copy slide diagram: From CO2 C6H12O6 o o o o CO2 has very ____________________ o ____________________ C6H12O6 contains a lot of chemical energy o ____________________ Synthesis = endergonic process o put in a lot of energy Reduction of CO2 C6H12O6 proceeds in many small uphill steps o each catalyzed by a specific enzyme o using _____________________________ From Light reactions to Calvin cycle o o Calvin cycle o chloroplast stroma Need products of light reactions to drive synthesis reactions o ATP o NADPH To G3P and Beyond! o o o ________________________________ o __________________________ o energy rich ______________________ o __________________________” G3P is an important intermediate G3P _______________ carbohydrates _____________ phospholipids, fats, waxes _____________ proteins _____________ DNA, RNA RuBisCo o Enzyme which fixes carbon from air o ribulose bisphosphate carboxylase o the most important enzyme in the world! it makes life out of air! o definitely the most abundant enzyme Accounting The accounting is complicated o 3 turns of Calvin cycle = 1 G3P o _____________________________ o ___________________________________ o 6 CO2 1 C6H12O6 (6C) o __________________________________ o any ATP left over from light reactions will be used elsewhere by the cell Photosynthesis summary o Light reactions o produced ATP o produced NADPH o consumed H2O o produced O2 as byproduct o Calvin cycle o consumed CO2 o produced G3P (sugar) o regenerated ADP o regenerated NADP Putting it all together: o Plants make both: o ______________ ___________________ o ____________________ Energy cycle: Explain “The Great Circle of Life” Summary of photosynthesis o o o o o o o o o o Where did the CO2 come from? _____________________ Where did the CO2 go?___________________________ Where did the H2O come from?____________________________ Where did the H2O go?_________________________________ Where did the energy come from? __________________________ What’s the energy used for?______________________________ What will the C6H12O6 be used for?______________________________ Where did the O2 come from?_______________________________ Where will the O2 go?_______________________________ What else is involved…not listed in this equation?_______________________- Supporting a biosphere On global scale, photosynthesis is the most important process for the continuation of life on Earth o o each year photosynthesis… o ______________________________ o _________________________________ heterotrophs are dependent on plants as food source for fuel & raw materials The poetic perspective… o o All the solid material of every _____________ was built by sunlight out of thin air All the solid material of every ____________ was built from plant material