Photosynthesis and Cellular Respiration Objectives Section 1 1. I can explain in detail the flow of energy through living systems 2. I can compare the chemical processes of autotrophs and heterotrophs 3. I can describe the role of ATP in metabolism 4. I can describe how energy is released by ATP. Metabolism Metabolism: all of the chemical reactions that take place within an organism • Metabolic pathways alter molecules in a series of steps. • Enzymes selectively accelerate each step. – enzymes are regulated to maintain a balance of supply and demand. • Catabolic reactions give off energy by breaking down complicated molecules to simpler compounds. • Anabolic reactions use energy to build complicated molecules from simpler compounds. • The energy released by catabolic pathways is used to drive anabolic pathways. Autotrophs vs Heterotrophs • Autotrophs make their own food through anabolic reactions – Many autotrophs carry out photosynthesis • Heterotrophs can not make their own food – Heterotrophs must eat other organisms to obtain energy from food through catabolic reactions – Heterotrophs utilize cellular respiration ATP • ATP powers cellular work • A cell does three main kinds of work: – Mechanical work, beating of cilia, contraction of muscle cells, and movement of chromosomes – Transport work, pumping substances across membranes against the direction of spontaneous movement – Chemical work, driving endergonic reactions such as the synthesis of polymers from monomers • ATP (adenosine triphosphate) is a type of nucleotide consisting of the nitrogenous base adenine, the sugar ribose, and a chain of three phosphate groups. • The bonds between phosphate groups can be broken by hydrolysis. So what? • Energy is stored in these bonds. • So? • The breaking of the chemical bond releases the energy ATP + H2O→ ADP + P + ENERGY Cellular Respiration • The process by which mitochondria break down glucose to make ATP • In order for our bodies to use the energy, the food must be broken down into a usable form – What is the usable form of energy? – What type of process breaks down molecules? Objectives Section 3 (yes, we skipped to section 3, for now) • I can summarize how glucose is broken down in glycolysis. • I can describe how ATP is made in cellular respiration. • I can identify the role of fermentation in cellular respiration. • I can evaluate the importance of oxygen in aerobic respiration. Overall Reaction C6H12O6 + 6O2 → 6CO2 + 6H2O + 38 ATP • Overall this is a three stage process 1. Glycolysis: • • Occurs in the cytoplasm Glucose is broken down 2. Krebs Cycle • • Breaks down pyruvate into CO2 Occurs in mitochondrial matrix 3. Electron Transport Chain • ATP is synthesized Glycolysis • Glyco = glucose Lysis = break down • Occurs in the cytoplasm • This stage occurs in BOTH aerobic and anaerobic respiration • Glucose breaks down into 2 pyruvate (2 ATP are also made) – Glucose is a 6-carbon sugar – Pyruvate is a 3-carbon molecule (there are two of them) – See diagram on simple explanation handout Glycolysis Reactants and Products Reactants • 1 glucose • Enzymes are needed • 2 ATP are needed to start Products • 2 Pyruvates (go to next step) • 4 ATP (2 are gained) • 2 NADH (go to ETC) Intermediate step • Pyruvate is converted to acetyl CoA • Occurs in the cytoplasm • See diagram on simple explanation handout Intermediate step Reactants and products Reactants • 2 pyruvate (from glycolysis) Products • 2 Acetyl CoA (go to next step) • 2 CO2 (given off as waste) • 2 NADH (go to ETC) Krebs Cycle (Citric Acid Cycle) • Occurs in the matrix of mitochondria • A series of reactions occur (this is not just one step) • Main purpose is to generate electrons for use in ETC • 2 ATP is given off • See diagram on simple explanation handout Krebs Cycle Reactants and Products Reactants • 2 Acetyl CoA • • • • Products 2 ATP 6 NADH (go to ETC) 2 FADH2 (go to ETC) 4 CO2 (given off as waste) Electron Transport Chain • Occurs in inner membrane of mitochondria • Series (chain) of coupled redox reactions (electrons are transported through the chain) • Electrons carried to this step by NADH and FADH2 (produced in previous steps) • Oxygen is used in this step • Water is given off Electron Transport Chain Reactants and Products Reactants • 10 NADH • 2 FADH2 • Oxygen Products • 34 ATP • H2O Where do we get 38 ATP? • 2 ATP made in glycolysis • 2 ATP made in Krebs Cycle • 34 ATP made in ETC – 1 NADH = 3 ATP o 10 X 3 = 30 – 1 FADH2 = 2 ATP o2 X 2 = 4 Anaerobic Respiration (Fermentation) • Glycolysis • Yields 2 pyruvate and 2 ATP • With no oxygen present, cellular respiration does not occur • ONLY 2 ATP ARE PRODUCED (compare to aerobic respiration) Two Types of Fermentation Alcoholic Fermentation • Pyruvate converted to ethyl alcohol and CO2 • Carried out by yeast and some bacteria • Used in producing alcohol (both consumable and for ethanol), and for baking Lactic Acid Fermentation • Pyruvate converted to lactic acid • Carried out by muscles when working hard (muscles need ATP but can’t get O2 ) • Causes muscle soreness and cramps Photosynthesis • Carried out by most (not all) autotrophs • 6CO2 + 6H2O + light energy→ C6H12O6 + 6O2 • Basically this reaction is the OPPOSITE of cellular respiration • See simple explanation handout and text Steps of Photosynthesis 1. Light reaction (depends on light) • • • Traps sunlight Produces electrons and ATP required to power the dark reaction Oxygen given off here 2. Dark reaction, aka Calvin Cycle (does not directly depend on light) • Uses ATP and electrons from light reaction and CO2 to make glucose See diagram on simple explanation handout