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Starting a Biotech Program
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Instructors
Stan Hitomi
Coordinator – Math & Science
Principal – Alamo School
San Ramon Valley Unified School District
Danville, CA
Kirk Brown
Lead Instructor, Edward Teller Education Center
Science Chair, Tracy High School
and Delta College, Tracy, CA
Bio-Rad Curriculum and Training Specialists:
Sherri Andrews, Ph.D.
sherri_andrews@bio-rad.com
Leigh Brown, M.A.
leigh_brown@bio-rad.com
Damon Tighe
damon_tighe@bio-rad.com
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Why Teach
Biotechnology?
An opportunity to teach science in a
way that results in
– Science literacy
– Technological skills
– Career awareness
– Critical thinking
– Student engagement
Students see the direct application of
– Biology
– Chemistry
– Physical science
– Math
Students learn
– Work ethics
– Team work, collaboration skills
– Communication skills, technical writing
– Laboratory techniques
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Things to
consider before
starting a
Biotechnology
Program
What type of program are you
interested in?
1. Add activities to present curriculum
2. Start a new course in the science
department
– Semester
– Year
3. Academy/Pathway
– CTE Program
– Project/Problem based
– 1 – 4 years
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Things to
consider before
starting a
Biotechnology
Program
What kind of course/program are you
looking to start?
-Technical
- Basic science
- manufacturing/career based/skills Based (CTE)
Who is your target student population?
- High School
- Tech Prep
- ROP/CTE
- College/Entry level training
Duration
- One semester, one year, two year
- Pathway, Program, Academy
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Things to
consider before
starting a
Biotechnology
Program
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What are the goals and objectives?
Partnerships
…you are
not alone
Create an advisory board to help develop
your program: Who can I ask for advice?
Partner with other educators and schools
and programs: Who is in my region?
- Community College
- Universities
- Local industry
(2+2+2 programs)
Partner with local industry and potential
internship sites
- Pharmaceutical R&D,QC
- Manufacturing R&D,QC
- Government Funded Agencies
- Academic labs/Medical Facilities
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Developing
Curriculum
What curriculum will you use?
Existing curriculum
•DNA Science (ISBN 0-87969-636-2)
•Molecular Biology & Biotechnology (978-1-5558-471-7)
•Biology and Biotechnology (1-55581-304-6)
•Biotechnology (0-7638-2282-5)
Modeled after another program
•AP Biology (Cindy Gay, CO)
•Kirk Brown website (www.jkirkbrown.com)
•George Cachianes
(http://www.lincolnhigh.net/academy/biotech/overview)
Build your own, Combo
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Why
reinvent the
wheel?…
Some model
programs:
High School Programs:
Kirk Brown, Tracy High School, Tracy, CA
Xan Simonson, Mesa HS Biotech Academy, Mesa, AZ
George Cachianes, Lincoln High School, San Francisco, CA
Holly Welch Choffin Career and Technical Academy Youngstown, PA
Eric Dybas Spanish River HS Boca Raton, FL
June Carmelego Santa Fe HS Alachua, FL
Community Colleges:
Trish Phelps, Austin CC
Diana Spencer, Tulsa CC
Bill Woodruff, Alamance CC
Bridgette Kirkpatrick, Colin County CC
Jim DeKloe, Solano CC
Kate Levine, David Palmer, Contra Costa CC
Elaine Johnson, City College of San Francisco, Bio-Link National
Center www.bio-link.org
University/Research Institution Outreach Programs
Kristi DeCourcy, Fralin Biotech Institute
Virginia Balke, Delaware Technical and Community College
Angela Eeds, Vanderbilt University
Nancy Hutchison, Science Education Partnership, Fred Hutch
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Developing
Curriculum
Focus of the Course
• Textbook based course
• Lab based course
• Independent projects
• Combination
Focus within the curriculum
• Health/Biomedical
• ROP/CTE
• Ag Science
Possible tools to implement to help your
students
• Specific skills
• Independent research
• Internships (summer or school-year)
• Job shadowing opportunities
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Developing
Curriculum
What knowledge and skills do I want my
students to have?
Examples
• Learn to use equipment safely and appropriately
• Learn sterile technique
• Know how to make solutions
• Keep a lab notebook
• Know structure and function of DNA
• Know how to manipulate DNA
• Understand enzyme structure and function
What training or type of degree or
certification is needed for local and state
requirements?
•Professional Development
•Certification programs
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Lab Activities
for existing
curriculum
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Sample Syllabi
Kirk Brown, Tracy High School
http://www.tracy.k12.ca.us/thsadvbio/
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Funding
Collaborations with University and Outreach
programs
- NSF and HHMI grants to Universities, Community
Colleges, Research Institutes have an outreach
component (Partnering institutions)
Other Grants or Funding Sources:
- Toshiba Tapestry Grants
- State Grants/CTE
- Local companies
- National companies
Target, Starbucks, McDonalds
Fundraising Activities:
- Family Science Night
- Genes in a Bottle™
explorer.bio-rad.com
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Teaching Resources
- Grant Writing Information
- Grantors and Directories
Finding Funds for Biotechnology Studies
Grant Writing Workshop
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Cloning and Sequencing Explorer Series
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Advanced
Comparative Proteomics Kit I: Protein Profiler Module
Comparative Proteomics Kit II: Western Blot Module
PV92 PCR Informatics Kit
GMO Investigator™ Kit*
Real-Time PCR Applications
Intermediate
Biofuel Enzyme Kit - NEW
ELISA Immuno Explorer™ Kit
Forensic DNA Fingerprinting Kit
Analysis of Precut Lambda DNA Kit
Restriction Digestion & Analysis of Lambda DNA Kit
pGLO Kit SDS-PAGE Extension
Crime Scene Investigator PCR Basics™ Kit*
Introductory
Genes in a Bottle™ Kit
pGLO™ Bacterial Transformation Kit
Green Fluorescent Protein Chromatography Kit
Secrets of the Rainforest™ Kit
Size Exclusion Chromatography Kit
Got Protein?™ Kit
Microbes and Health Kit
Building
Step by Step
Equipment
• What equipment do I have?
• What equipment do I need?
Equipment wish list:
• What is my budget?
Year to Year Growth Plan:
What equipment will allow you to
grow your program with time?
(Think long-term)
Year 1, 2, 3 …
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Complete
Biotechnology
Teaching
Resources
Standards Aligned Kit Based Lab Activities
•Materials for 8 complete student workstations
Detailed Curriculum Manuals and Teaching Guides
•Teacher guide/Student guide
•Step-by-step preparation and set-up
•Inquiry-based adaptable lessons to match grade
and skill level and maximize student involvement
•Extension activities
Teaching Resources
•PowerPoint Presentations
•Animations
•Resource Links
•CTS
•explorer.bio-rad.com
Professional Development/Training
•CTS
Research Grade Equipment
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Technical Support
The Pipeline
PRIMARY/SECONDARY EDUCATION
HIGHER EDUCATION
STIMULATE & DEVELOP
INTEREST IN SCIENCE
SKILLS, APPLICATIONS,
CAREER OPTIONS
Elementary/
Middle School
High School
Community/
vocational
college
University
RESEARCH INSTITUTIONS
BASIC & APPLIED
RESEARCH
Academic
Industry
LIFE SCIENCE CAREER PATH
Kits & equipment
Lab extensions
Kits & equipment
Lab extensions
Student research
Real-Time PCR
Proteomics: 2D Gel Electrophoresis
Chromatographic separations
Need Help?
Your CTS can
help you!
Curriculum Training Specialists
Essy_Levy@Bio-Rad.com
(858) 201-9301
Sherri_Andrews@Bio-Rad.com
(510)314-3748
Professional_Development@bio-rad.com
http://explorer.bio-rad.com
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Genes in a Bottle
Students extract and
preserve their own DNA
LIFE SCIENCE STANDARDS & SKILLS:
Cellular structures
– membrane, organelles
DNA
– location, solubility
– genetic information
– extraction from tissue
Enzyme properties & function
– use of protease to aid in extraction
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pGLO™ Bacterial
Transformation
Students transfer a
jellyfish gene to
bacteria
Can regulate the
expression of GFP
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LIFE SCIENCE STANDARDS & SKILLS:
Central Dogma of Molecular Biology
– DNA>RNA>Protein>Trait
– Genetic engineering
Prokaryotic cells
– Cell biology, genetic transmission
– Antibiotic resistance
Microbiology techniques
– Sterile technique
– Growth media
Chromatography
Kits:
GFP Chromatography
purify the bioluminescent
protein (extension to
pGLO)
Secrets of the
Rainforest™
study the ethical issues of
biotechnology
Size Exclusion
Chromatography
Separate vitamin B12 and
hemoglobin using
chromatography
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LIFE SCIENCE STANDARDS & SKILLS:
Chromatography
– Protein properties
– Tools for protein purification
Creating a commercial product
– Scientific, ecological, ethical and legal issues of biotechnology
Biomolecules
– Physiological function
– Naturally pigmented molecules
– Maintenance of deleterious alleles (e.g. sickle cell anemia)
Microbes and
Health –
“What Causes
Yogurtness?”
Isolate the
microorganisms from
yogurt and inoculate
milk, linking the “sick”
milk, to the causative
microbe
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LIFE SCIENCE STANDARDS & SKILLS:
Isolate yogurt-causing bacteria
– Follow Koch’s postulates
– Microbiology and human disease
– Biotechnology and food production
Learn laboratory microbiology skills
– Study food microbiology and bacterial metabolism
Inquiry Based
– Do different brands of yogurt contain different microbes?
– Directly linked to students daily lives
Forensic DNA
Fingerprinting
Compare Crime Scene
DNA with 5 Suspects to
determine
“Who Done it?”
Restriction
Analysis of Lambda
DNA
Use restriction enzymes
and gel electrophoresis
to analyze DNA
LIFE SCIENCE STANDARDS & SKILLS:
Allelic differences in populations
– DNA as a unique identifier
– Heredity
Restriction enzymes
– Function and properties
– Origin in bacteria and use in genetic engineering
– Plasmid mapping
Agarose Gel Electrophoresis
– Separation of molecules using electrical current
– Size based on distance traveled, creating buffers
Micropipetting techniques
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Crime Scene
Investigator
PCR Basics™
Students learn to use the
polymerase chain
reaction (PCR) and DNA
electrophoresis to
determine the genotypes
of 5 DNA samples
Simulate real-world crime
lab techniques using one
of the Short Tandem
Repeat (STR) loci
commonly used in
forensic typing.
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LIFE SCIENCE STANDARDS & SKILLS:
Allelic differences in populations
– DNA as a unique identifier
– Use of PCR in DNA profiling
– Polymorphic loci and multiple alleles
– Non-coding DNA sequences
Agarose Gel Electrophoresis
– Separation of molecules using electrical current
– Size based on distance traveled, creating buffers
Micropipetting techniques
PV92 PCR
Informatics
Students isolate and
examine the DNA
fingerprint of their
own DNA
LIFE SCIENCE STANDARDS & SKILLS:
Population Genetics
– Hardy Weinberg equilibrium
– Evolution, migration, speciation
– Bioinformatics using Cold Spring Harbor allele server
Polymerase Chain Reaction
– Uses in forensics, archeology, research, disease
diagnostics
Agarose Gel Electrophoresis
– Separation of molecules using electrical current
– Size based on distance traveled, creating buffers
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GMO
Investigator™
Students extract and
amplify DNA from
grocery store foods
LIFE SCIENCE STANDARDS & SKILLS:
Agriculture and environment
– Pesticides and herbicides
– Population growth and environmental challenges
– Plant biodiversity and ecosystems
Molecular Biology
– DNA replication and PCR
– Genetic transformation to create GMOs
– Control of gene expression (in foreign hosts)
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Got Protein?™
Kit
Determine the protein
concentration in
common beverages
such as milk, sports
drinks and more
Engages students –
they bring in their
samples of choice
LIFE SCIENCE STANDARDS & SKILLS:
Proteins
– Essential molecules in all living cells
Bradford Assay
– Coomassie Blue dye binds to protein and allows for
quantitation and detection
– correlation between the amount of blue color and the amount
of protein
Spectrometry
– Use of spectrophotometer
– Apply Beer’s Law
Inquiry based - which foods/drinks provide more
proteins?
– Directly links to students daily lives
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ELISA Immuno
Explorer™
Students simulate
spreading an
infectious disease
and detect origin
Types of ELISA Tests included:
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Tracking the spread of a
disease (epidemiology)
•
Detecting specific
antigens in a sample
•
Diagnosing past exposure
to a disease
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LIFE SCIENCE STANDARDS & SKILLS:
Immunology
– antigen-antibody interaction
– virology
– infectious diseases
Enzyme Properties
– enzyme-substrate interaction
– colorimetric detection
Real world applications
– Pregnancy, drug, HIV testing
– GMO and environmental testing
– bioterrorism
Comparative
Proteomics Kits:
Protein Profiler –
Module
Evolution Wet Lab –
create protein profiles
for different fish
species
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LIFE SCIENCE STANDARDS & SKILLS:
Molecular evolution
– Natural selection and genetic diversity
– classification
Polyacrylamide Gel Electrophoresis
– protein separation techniques
Module 1
Comparative
Proteomics Kits:
Western Blot –
Module
Takes Protein Profiler
to the Next Level –
identify the specific
protein using
antibodies
LIFE SCIENCE STANDARDS & SKILLS:
Molecular evolution
– Natural selection and genetic diversity
– classification
Western blotting
– Explore immunodetection
– Protein separation techniques
– Antibodies as tools
Module 2
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Biofuel Enzyme
Kit
Guides instruction on
enzyme kinetics and
biofuel energy
sources
LIFE SCIENCE STANDARDS & SKILLS:
• Enables both qualitative and quantitative
measurements of reactions
- Determine the rate of reaction in the presence or absence of
an enzyme
- Determine the effect of temperature, pH, enzyme
concentration, substrate concentration on the rate of reaction
- Test the ability of mushroom extracts to increase the rate of
reaction
• Aligns with AP Biology Lab 2
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Cloning and
Sequencing Series
Genomic DNA Extraction
DNA Precipitation
DNA Quantitation
Bioinformatics
Sequence Data Editing
Contig Assembly
Intron-Exon Prediction
GAPDH PCR
Nested PCR
Degenerate primers
Exonuclease
Sequencing
Automated sequencing
Gel Electrophoresis
DNA Gel Interpretation
Band Identification
Standard Curve Use
Plasmid Miniprep
Restriction Enzyme
Digestion
Gel Electrophoresis
PCR Purification
Size Exclusion Chromatography
Microbial Culturing
Antibiotic Selection
Sterile Technique
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Cloning
Direct PCR cloning
Transformation
Ligation
Webinars
• Enzyme Kinetics — A Biofuels Case Study
• Real-Time PCR — What You Need To Know
and Why You Should Teach It!
• Proteins — Where DNA Takes on Form and
Function
• From plants to sequence: a six week
college biology lab course
• From singleplex to multiplex: making the
most out of your realtime experiments
explorer.bio-rad.comSupportWebinars
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