General Biology Biotechnology Study Guide
Mrs. Zurawski
VOCAB: Study the following vocab. Place a next to each word when you can define it from memory.
____ Gene
____ Vector
____ Biotechnology
____ GMO (genetically modified organism)
____ Transgenic Organism
____ Gene therapy
____ DNA Ligase
____ DNA fingerprint
____ Restriction Enzyme
____ The Human Genome
____ Recombinant DNA
____ Sticky Ends
____ Plasmid
____ Genetic Engineering
____ Gel Electrophoresis
____ Palindrome
REVIEW QUESTIONS:
1. What is a vector and what are a few examples?
2. How are the following terms related:
Transgenic Organism
3.
Recombinant DNA
Biotechnology
What is a plasmid? How are they useful in biotechnology?
4. What is genetic fingerprinting/gel electrophoresis used for?
5. What is the role of restriction enzymes in gel electrophoresis?
6. What is the role of restriction enzymes in making recombinant DNA?
7. What enzyme is used to attach sticky ends of DNA?________________________________________
8. What is the human genome?
9. What is the difference between genetic engineering and gene therapy?
10.
Which of the following DNA sequences would most likely be a restriction enzyme site? Why?
a. CTCTC
11.
b.
CCGGCC
c. GAATTC
d. CTGCAG
Who’s the daddy of this child? (call the 1st lane “alleged father 1” & the 2nd lane “alleged father 2”)
How do you know?
Use the gel to the right to answer the questions.
12. At which of these lettered bands would you find the longest
piece of DNA? How do you know?
A
D
13. At which of these lettered bands are there many fragments
of that size? How do you know?
B
C
14.
Jake and Emma's four children were grown and had families of
their own. Jake was murdered, and the police suspected his oldest child
(#1) as the culprit, who had unexplained scratches on his arms and face.
DNA was extracted from under the victim's fingernails, and that was
compared with those of the other members of the family.
Be a real Sherlock Holmes: was the culprit really the oldest child?