Lab # 7 Body Movements and Muscle Histology OBJECTIVES 1- Describe and demonstrate the different types of body movements. 2- List the three types of muscle tissue and function of each. 3- Describe the histological appearance of each type. 4- Describe the organization of the skeletal muscle. 5- Describe the microanatomy of a muscle fiber. 6- Understand the rules that determine the name of some muscles. Flexion, Extension and Hyperextension Flexion: Movement that decreases the joint angle in hinge joints. Extension: Movement that straightens a joint and generally returns a body part to the zero position. Hip flexion Flexion Knee flexion Extension Lateral flexion Hyperextension: Further extension of a joint beyond the zero position. Flexion and extension occur at nearly all diarthroses, hyperextension is limited to a few joints. Hyperextension Hyperextension Extension Flexion Abduction: Movement of a body part in the frontal plane away from the midline of the body. Adduction: Movement of a body part in the frontal plane toward the midline of the body. Elevation: A movement that raises a body part vertically in the frontal plane. Protraction: The anterior movement of a body part in the transverse (horizontal) plane. Depression: A movement that lowers a body vertically part in the frontal plane. Retraction: The posterior movement of a body part in the transverse (horizontal) plane. Supination: Forearm movement that turns the palm to face anteriorly or upward. The forearm is supinated in anatomical position (the radius is parallel to the ulna) Pronation: Forearm movement that turns the palm to face posteriorly or downward. The radius spins on the capitulum of the humerus. The head spins in the radial notch of ulna and the radius crosses stationary ulna like an X Tendons: Narrow bands of connective tissue that connect muscles to bone TENDONS Ligaments: Bands of connective tissue that join bone to bone LIGAMENT Aponeuroses: Bands of connective tissue that attach flat muscle to another muscle or to several bones APONEUROSIS Epicraneal aponeuroses ( Galea ) Lumbar aponeuroses Abdominal aponeuroses Muscle Tissue MARTINI page 133 DIFFERENT TYPES OF MUSCLE 1- Skeletal muscle 2- Cardiac muscle 3- Smooth muscle MUSCLE HISTOLOGY Types of muscle: Cell characteristics 1- Skeletal Long, Cylindrical, Striated, Multinuclear 2- Cardiac Short, Branched, Striated, Single nucleus 3- Smooth Single nucleus Short, Spindle, Nonstriated, Types of Muscle Tissue Cell characteristics Nuclei Long, cylindrical, unbranched striated , multinuclear Skeletal muscle Nucleus Striations Intercalated disk Cardiac muscle Nucleus Smooth muscle Short, branched, striated , single nucleus, intercalated discs Nerve ending Short, spindle-shaped, non-striated , single nucleus Organization of the Skeletal Muscles (Unit 13, page 173) Skeletal Muscle Epimysium Fascicle Perimysium Muscle fiber (cell) Endomysium Sarcoplasm Capillary Nucleus Mitochondria Sarcolemma Myosatellite cell Endomysium They are involved in the repair of damaged muscle Myofibrils They consist of bundles of myofilaments (thin filaments and thick filaments) Axon MUSCLE FIBER (cell) SKELETAL MUSCLE Contains: Surrounded by: Muscle fascicles Epimysium MUSCLE FASCICLE Contains: Surrounded by: Muscle fibers (cells) Perimysium MUSCLE FIBER (CELL) Contains: Surrounded by: Myofibrils Endomysium MYOFIBRIL Contains: Myofilaments MYOFILAMENTS Thick filaments: myosin Sarcomere Thin filaments: actin They are organized in sarcomeres Structure of the Skeletal Muscle Fiber Mitochondria Terminal cisterna They produce the chemical energy (ATP) for muscle contraction. Sarcolemma Sarcoplasm Thin filament Myofibril Thick filament Triad Sarcoplasmic reticulum They conduct the nerve impulse It stores calcium for T tubules from the sarcolemma to the muscle contraction. interior of the cell. Sarcomere Structure Z line They consist of proteins called actinins, which interconnect thin filaments of adjacent sarcomeres. Z line Sarcomere They are the smallest functional units of the muscle fiber M MYOFIBRIL line I band (It contains thin filaments but not thick filaments Zone of overlap H band Zone of overlap A band Myosin Actin (thick filaments) (thin filaments) M line: It consists of proteins that connect the each thick filament with its neighbors. I band Z line A band: Its length is Zone of overlap H band M line equal to the length of the thick filaments. It contains both thin and thick filaments. H band: It is a lighter region on either side of the M line, which contains only thick filaments. Zone of overlap: It is the region where the thin filaments are situated between the thick filaments. Sarcomere Structure I band A band Z line H zone Zone of overlap M line Thick filament Sarcomere Z line Thin filament The Neuromuscular Junction Motor neuron Axon of motor neuron Axon terminal It carries the nerve impulse. It releases the neurotransmitter. Neuromuscular junction It is the point where the motor neuron and the muscle fiber meet. Muscle cell or fiber Nucleus Myofilaments Sarcolemma Myofibril (They are organized in sarcomeres) Axon Terminal or Synaptic Knob Smooth E.R. Mitochondrion They produce the ATP for active transport of ions Synaptic vesicles They contain the neurotransmitter Synaptic vesicles releasing the neurotransmitter Neuromuscular Junction and Muscle Cell or Fiber Terminal (T) tubules They carry the nerve impulse inside the muscle cell Sarcoplasmic reticulum Myofibril It stores calcium for muscle contraction Two terminal Triad cisternae and one T tubule Terminal cisternae They store calcium for muscle contraction Mitochondria They provide the energy (ATP) for muscle contraction Myelin sheath Motor end plate Synaptic cleft Sarcolemma Endomysium Axon of the Junctional folds motor neuron Synaptic It carries the vesicles nerve impulse They contain the neurotransmitter Endomysium Myofilaments Sarcolemma Sarcoplasm Neuromuscular Junction and Muscle Cell or Fiber Triad Two terminal cisternae and one T tubule Myofilaments Sarcoplasmic reticulum It stores calcium for muscle contraction Terminal cisternae Transverse (T) tubules Synaptic cleft AXON TERMINAL Mitochondria They provide the energy (ATP) for muscle contraction Synaptic vesicles They contain the neurotransmitter Junctional folds They carry the nerve impulse inside the muscle cell Sarcolemma Axon of the motor neuron It carries the nerve impulse Endomysium Myelin sheath Neuromuscular Junction and Muscle Cell or Fiber Sarcomeres Superior view Myofibrils Neuromuscular junction Axon terminal Nuclei They release the neurotransmitter Axon of the motor neuron Sarcolemma It carries the nerve impulse Sarcoplasm Endomysium Microstructure of the Muscle Fiber Terminal They store calcium for cisterna muscle contraction Thick myofilaments (Myosin) Thin myofilaments (Actin) T tubule They carry the nerve impulse inside the muscle cell Triad Two terminal cisternae and one T tubule Sarcoplasmic reticulum It stores calcium for muscle contraction Mitochondrion They provide the energy (ATP) for muscle contraction