MSP Science Lesson – 5.3 Cells GPS Standard: S5L3. Students will diagram and label parts of various cells (plant, animal, single-celled, multi-celled). a. Use magnifiers such as microscopes or hand lenses to observe cells and their structure. b. Identify parts of a plant cell (membrane, wall, cytoplasm, nucleus, chloroplasts) and of an animal cell (membrane, cytoplasm, and nucleus) and determine the function of the parts. Essential Question: What do all eukaryotic cells have in common? How do plant and animal cells differ? What are the functions of the cellular organelles? Engagement: (Find out what teachers already know) Venn diagram (compare/contrast) Plant Cells Animals Cells All Cells Another idea could be to begin a concept map. I like to do this as an engagement activity with students in groups. As students progress through an activity they add information using sticky notes (“parking lot”). As a culminating assessment, each group does a final concept map. See addendum to this activity. Exploration I: http://www.southerncompany.com/learningpower/pdf/Lifedrops_A%20Pond%20Water%20Study.pdf Materials (per group): Microscopes, slides, cover slips, water, dropper, iodine and/or methylene blue stain, onions, Elodea (available at pet stores, aquarium section), pond water or hay infusion liquid, flat tooth picks, other assorted samples for viewing or prepared slides that might include some non-living samples like sand, salt crystals, etc. Note: For teachers without microscopes, slides 1 – 6 of PPT show may be used. Directions Teacher should demonstrate how to make wet mount and dry mount slides. A wet mount slide includes the following: a slide, a cover slip, a specimen, a drop of stain or water. When preparing a slide, hold the cover slip at an angle and let it drop onto the slide slowly trapping the specimen between the two pieces of glass. A piece of onion skin is easy to use in this first activity. Soak onion sections the night before in water. Get into groups of 4. Read through the different wet mounts that you need to make and decide who will prepare each one. Each person in the group will take a turn preparing a wet mount and sharing it with everyone else in the group. Carefully follow the instructions for each sample. Everyone in the group will draw what each sample looks like on their own piece of paper and give a brief description of the unique characteristics that they see in that sample (i.e. color, movement, size, thickness, etc.). Also, decide as a group, if the specimen is animal or plant. Elodea Leaf: Prepare a wet mount of an Elodea leaf by tearing a single leaf from the stem and placing it on a slide with a drop of water and a cover slip. Observe the leave under low and high power. Onion epidermis: With tweezers or finger tips, remove the inner surface of a section of an onion. (It will look very thin, like saran wrap.) Make a wet mount of the onion skin and observe it under low and high power. Try adding a drop of iodine stain to the slide and re-examine the cells. Cheek: Scrape GENTLY the inside of your cheek with the flat end of a toothpick (DO NOT DRAW BLOOD!) and rub the toothpick onto a slide. Go to a sink and add one drop of methylene blue to the slide and cover with a cover slip. Caution! Methylene blue stains so don't get any on you! Using an eyedropper, rinse the excess methylene blue into the sink. You should see tiny blue specks on the slide. These are your cheek cells. Dry the bottom of the slide. Examine under low and high power. Pond Water: Place one drop of pond water on a slide and put a cover slip over it. Turn the light down on your microscope and search for living organisms. How do you know that what you are looking at is living? Cell Comparison Lab Student Observations Procedure: * Draw the cells you see from your microscope field in the circles below. * Label on the first line whether it is a Plant or an Animal cells. * Explain what distinguishing characteristic(s) makes them a plant or an animal cell. Summarize: Describe briefly what was similar about all of the cells you viewed. Describe what was different. Alike – Different – Explanation I: (Interpret the observations) 1. Plant cells have a cell wall, but animal cells do not. 2. Plant cells have chloroplasts, but animal cells do not. 3. Plant cells generally have a more rectangular shape because the cell wall is more rigid. Animal cells have a round or irregular shape because they do not have a cell wall. 4. Plants cells usually have one or more large vacuole(s), while animal cells have smaller vacuoles, if any are present. Animal Cell Plant Cell An organelle is a small structure within the cell (organelle literally means 'tiny structure'). Some examples of cell organelles are the cell wall, cell membrane, and nucleus. The following are the major functions of organelles: barrier between cell and its environment protection/support building and repairing of cell parts transport of materials storage and release of energy disposal of waste materials reproduction (increase in number) Exploration II – Organelles Using PowerPoint slides 11 (or go straight to Cells Alive interactive site for comparing plant and animal cells) and 12 (table of organelles), students can investigate function of cellular organelles and continue comparison of plant versus animal cells. This can be a whole class activity (1 computer classroom), done in small groups (several computers), or as an individual activity (computer lab). Explanation II – http://www.usoe.k12.ut.us/curr/science/sciber00/7th/cells/sciber/orgtable.htm For completed table of cellular organelles Extension: If you’re into technology and microorganisms – A virtual pond dip http://www.microscopy-uk.org.uk/index.html?http://www.microscopy-uk.org.uk/ponddip/index.html If you’d like to set up an in-class investigation of microorganisms: http://www.bottlebiology.org/ If you’ve already used duckweed in your investigation, here are several activities, most related to ecology, you might try: http://www.mobot.org/jwcross/duckweed/education.htm Everyday Applications Beneficial (waste water treatment; oil spill clean-up) and harmful bacteria (infections, disease) – tie in to standard S5L4 Genetics – tie in to standard S5L2 Environmental science applications using a familiar organism http://www.mobot.org/jwcross/duckweed/education.htm Cross Curriculum Connections History/technology: Students will research historical events leading to the development of the cell theory. o Research should include contributions made by the following people/scientists -Robert Hooke, Hans and Zacharias Janssen, Anton van Leeuwenhoek, Matthias Schleiden, Theodor Schwann, Rudolph Virchow, etc. and dates of their contributions. 2. Students will report on their findings by constructing a timeline showing the chronology of the 1. Resources What to do if you don’t have microscopes: http://comm.nsdl.org/download.php/915/NSDL_WS7_Microscopes.pdf Everything you need for a cell comparison study: http://waynesword.palomar.edu/lmexer1a.htm Cell photographs: http://www.usoe.k12.ut.us/curr/science/sciber00/7th/cells/sciber/cellphot.htm Great cell unit: http://www.usoe.k12.ut.us/curr/science/sciber00/7th/cells/sciber/intro.htm Cell organelles – table w/ functions: http://www.usoe.k12.ut.us/curr/science/sciber00/7th/cells/sciber/orgtable.htm Tons of cell sites: http://classroom.jc-schools.net/sci-units/cells.htm Interactive plant and animal cells; illustrations to download http://www.cellsalive.com/ Cell website with good basic cell facts and slides: http://library.advanced.org/3564/ Amazing microscopy from England: http://www.microscopy-uk.org.uk/micropolitan/index.html Cell facts; quizzes: http://www.biology4kids.com/files/cell_main.html Free registration; excellent science resources: http://www.teachersdomain.org/tdhome.html Evaluation: Participants … Mini quiz (PPT slides 7 – 10 http://www.usoe.k12.ut.us/curr/science/sciber00/7th/cells/sciber/intro.htm) Build a cell (PPT slide 13 (http://www.bioscope.org/taste/builda.htm) Complete Venn diagram or concept map Complete compare and contrast chart of organelles Culminating activity – Cells on the Ceiling http://www.accessexcellence.org/AE/AEC/AEF/1994/hopkins_cells.html