#1 NAME____________________________________
MCB 2004
GENERAL MICROBIOLOGY
TEST #1, FEBRUARY 5, 2001
IN THIS PART THERE ARE FORTY-FIVE QUESTIONS WORTH TWO POINTS EACH.
THERE IS ONLY ONE CORRECT ANSWER FOR EACH QUESTION. PLEASE CIRCLE
THE CORRECT LETTER OR PLACE THE CORRECT LETTER JUST TO THE LEFT OF
THE QUESTION NUMBER. THEN TRANSFER ALL YOUR ANSWERS TO THE ANSWER
SHEET AT THE END OF THE TEST PACKET.
KEY FOR QUESTIONS 1-7. THERE IS ONLY ONE CORRECT ANSWER BUT EACH KEY
WORD MAY BE USED MORE THAN ONCE (OR NOT AT ALL).
A. MITOCHONDRION
B.
C.
D.
GOLGI APPARATUS
NUCLEUS
PILUS
E. RIBOSOME
_____1. FOUND ONLY IN PROCARYOTES
_____2. COMPOSED OF THREE RNAs AND ABOUT 50 PROTEINS
_____3. FOUND IN BOTH PROCARYOTES AND EUCARYOTES
_____4. APPENDAGE USED FOR ATTACHMENT
_____5. THE SITE OF PROTEIN SYNTHESIS
_____6. THE LARGEST AND MOST OBVIOUS ORGANELLE IN EUKARYOTIC CELLS
_____7. ORGANELLE RESPONSIBLE FOR ENERGY PRODUCTION
8. IN THE GRAM STAIN THE COUNTER STAIN IS _______.
A. BASIC FUCHSIN
B.
C.
IODINE
CRYSTAL VIOLET
D. WATER
E. ACETONE OR ALCOHOL
9. STAINING FIXED CELLS ONLY WITH METHYLENE BLUE IS AN EXAMPLE OF:
A. SPECIAL STAINING

B. SIMPLE STAINING
C. BOTH OF THE ABOVE
D. NEITHER OF THE ABOVE
MCB 2004
TEST #1
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10. IN BRIGHT FIELD MICROSCOPY LIGHT PASSING THROUGH THE
SPECIMEN (ON THE STAGE) NEXT PASSES THROUGH THE
A.
B.
OBJECTIVE LENS
LIGHT SOURCE
C.
D.
CONDENSER
OCULAR LENS
11. THE ACID FAST "OUTER MEMBRANE" IS UNUSUALLY THICK BECAUSE OF THE
A. MYCOLIC ACIDS
B. LIPOPOLYSACCHARIDES
C. BOTH OF THE ABOVE
D. NEITHER OF THE ABOVE
12. THE EXPONENTIAL PHASE OF BACTERIAL GROWTH IS ALSO CALLED THE
A.
B.
C.
D.
E.
STATIONARY PHASE
LOG PHASE
LAG PHASE
DEATH PHASE
REPLICATION PHASE
13. MOST HUMAN PATHOGENS ARE
A. BAROPHILES
B. THERMOPHILES
C. BASOPHILES
D.
E.
MESOPHILES
PSYCHROPHILES
14. THE DOUBLING TIME OF E. COLI IN NUTRIENT BROTH AT 37 o C IS
30 MINUTES. THE INITIAL INOCULUM OF THE CULTURE CONTAINS 500
CELLS. AFTER TWO HOURS OF EXPONENTIAL GROWTH THE CULTURE CONTAINS
A. 1000 CELLS
B. 4000 CELLS
C.
D.
E.
5000 CELLS
8000 CELLS
10000 CELLS
15. A BACTERIUM THAT OXIDIZES SULFUR WITH OXYGEN FOR ENERGY AND

USES CARBON DIOXIDE AS ITS CARBON SOURCE IS A
A. CHEMOAUTOTROPH
B. STRICT ANAEROBE
C. BOTH OF THE ABOVE
D. NEITHER OF THE ABOVE
16. THE FOLLOWING IS ONE INGREDIENT OF A DEFINED MEDIUM
A. NUTRIENT AGAR
B. GLUCOSE
C. PEPTONE
D. YEAST EXTRACT
E.
MCB 2004
TEST #1
BEEF INFUSION
PAGE 3
17. HIS DISCOVERY IN 1953 EARNED HIM THE NOBEL PRIZE AND MARKED
THE BEGINNING OF THE AGE OF MOLECULAR BIOLOGY.
A. ROBERT KOCH
B. JAMES WATSON
C. VAN LEEUWENHOEK
D. EDWARD JENNER
E. LOUIS PASTEUR
18. PEPTIDOGLYCAN IS MADE OF POLYMERS OF TWO SUGARS CROSSED-LINKED BY
A. LIPIDS
B. PEPTIDE CHAINS
C. BOTH OF THE ABOVE
D. NEITHER OF THE ABOVE
19. IN 1800 50% OF THE U.S. POPULATION DIED OF INFECTIOUS DISEASES;
25% DIED OF
A. INFLUENZA
B.
C
D.
SMALLPOX
POLIO
TUBERCULOSIS
20. THE LIGHT MICROSCOPE IS CAPABLE OF ____ RESOLUTION, AND ____
MAGNIFICATION.
A. 0.4 mm, 10 X
B. 10 µm, 100 X
C. 0.2 µm, 2,000 X
D. 2 nm , 100,000 X
21. ONE METER EQUALS
A. 1000 mm
B. 1,000,000 µm

C.
D.
BOTH OF THE ABOVE
NEITHER OF THE ABOVE
22. THE DARKFIELD MICROSCOPE IS USEFUL FOR
A. SEEING BACTERIAL FLAGELLA
B.
C.
D.
DIAGNOSING SYPHILIS
BOTH OF THE ABOVE
NEITHER OF THE ABOVE
23. THE NUMBER OF BACTERIA PER ML IN THIS CULTURE FLASK IS
A. 42,000
B. 210,000
C. 420,000
D.
E.
MCB 2004
2,100,000
4,200,000
TEST #1
PAGE 4
KEY FOR QUESTIONS 24-30. THERE IS ONLY ONE CORRECT ANSWER, BUT EACH
ANSWER MAY BE USED MORE THAN ONCE (OR NOT AT ALL).
A. ESCHERICHIA COLI
B.
C.
D.
E.
STAPHYLOCOCCUS AUREUS
MYCOBACTERIUM LEPRAE
TREPONEMA PALLIDUM
CLAMYDIA TRACHOMATIS
_____24. CONTAINS LIPOPOLYSACCHARIDE (LPS)
_____25. ITS ENVELOPE HAS TWO LAYERS, A PLASMA MEMBRANE AND A THICK
PEPTIDOGLYCAN LAYER
_____26. IT HAS A CELL WALL THAT DOES NOT CONTAIN PEPTIDOGLYCAN
_____27. IT HAS A COILED SHAPE AND AXIAL FILAMENTS
_____28. ITS CLOSE RELATIVE IS PSEUDOMONAS AERUGINOSA
_____29. CONTAINS MYCOLIC ACIDS AND ARABINOGLACTANS IN ITS ENVELOPE
_____30. GROWS AS SPHERICAL CELLS IN CLUSTERS

31. KILLING ALL ORGANISMS INCLUDING SPORES PRESENT IN A MATERIAL
IS KNOWN AS
A.
B.
FILTRATION
INCINERATION
C. STERILIZATION
D. LYOPHILIZATION
E. PASTEURIZATION
32. MANY ANTIBIOTICS AND VITAMINS WHICH ARE UNSTABLE TO HEAT ARE
____________ BEFORE THEY ARE ADDED TO BACTERIAL GROWTH MEDIA
A.
B.
C.
D.
AUTOCLAVED
PASTEURIZED
FROZEN
FILTERED
E. BOILED
33. ORGANIC COMPOUNDS LIKE ISOPROPANOL PROBABLY HAVE THEIR GREATEST
BACTERIAL KILLING EFFECT BY
A.
B.
HYDROLYZING DNA
DESTROYING PROTEINS
C. DISRUPTING PLASMA MEMBRANES
D. DISRUPTING SPORES
E. DISRUPTING RIBOSOMES
MCB 2004
TEST #1
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34. HIS OBSERVATION THAT MILKMAIDS WERE IMMUNE TO SMALLPOX LED TO
THE FIRST USEFUL VACCINE
A. JONAS SALK
B. LOUIS PASTEUR
C.
D.
E.
ALEXANDER FLEMING
PAUL EHRLICH
EDWARD JENNER
35. EUCARYOTIC CELLS ARRESTED JUST BEFORE MITOSIS ARE IN
A. Go
B. G1
C. G2
D. M
E. S
36.
37. A BACTERIAL FLAGELLUM IS MADE UP OF THREE PARTS. THEY INCLUDE
A. THE HOOK

B. THE MICROTUBULES
C. BOTH OF THE ABOVE
D. NEITHER OF THE ABOVE
38. IF YOU WANTED TO STERILIZE PLASTIC-WARE (LIKE YOUR PLASTIC
PETRI DISHES OR DISPOSABLE PIPETS) YOU COULD USE
A.
B.
AUTOCLAVING
ETHYLENE OXIDE
C.
D.
BOTH OF THE ABOVE
NEITHER OF THE ABOVE
39. THE LARGE SUBUNIT OF THE BACTERIAL RIBOSOME CONTAINS
A. 23S RNA, 16S RNA AND 30 PROTEINS
B. 23S RNA, 5S RNA AND 30 PROTEINS
C. 16S RNA, 5S RNA AND 20 PROTEINS
D.
E.
16S RNA, 5S RNA AND 20 PROTEINS
16S RNA AND 20 PROTEINS
40. THE PORTION OF LIPOPOLYSACCHARIDE (LPS) MADE OF REPEATING SUGARS
IS THE
A. THE O ANTIGEN
B. THE CORE POLYSACCHARIDE
C. THE ENZYMATIC PORTION
D. LIPID A
E. THE CELL-BINDING PORTION
MCB 2004
TEST #1
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KEY FOR QUESTIONS 41-45. EACH QUESTION HAS ONLY ONE ANSWER, BUT EACH
ANSWER MAY BE USED MORE THAN ONCE (OR NOT AT ALL).
A. THE TCA CYCLE
B. PROTEIN SYNTHESIS
C. LACTIC ACID FERMENTATION
D. THE ELECTRON TRANSPORT CHAIN
E. HISTIDINE BIOSYNTHETIC PATHWAY
_____41. ANABOLISM OF AN AMINO ACID
_____42. IN THE FIRST STEP, OXALOACETIC ACID ACCEPTS ACETATE FROM
ACETYL-COA TO PRODUCE CITRIC ACID
_____43. ANABOLISM OF A MACROMOLECULE
_____44. THE OXIDATION OF NADH BY MOLECULAR OXYGEN

_____45. ONLY TWO ATPS ARE PRODUCED FROM ONE GLUCOSE IN THIS PROCESS
ANSWER ONE OF THE FOLLOWING THREE QUESTIONS (10 POINTS)
46. DRAW A TYPICAL BACTERIAL GROWTH CURVE AND LABEL IT (DON'T
FORGET TO LABEL THE AXES.).
OR
47. DRAW A DIAGRAM OF A GRAM NEGATIVE CELL ENVELOPE AND LABEL IT.
OR
48. DRAW AND LABEL THE METABOLIC PATHWAYS (DIAGRAMATICALLY) FROM
GLUCOSE TO CO
2
AND H
2
0 USING MOLECULAR OXYGEN. INCLUDE ATP
PRODUCTION.