NAME________________________________
SSN_____________________
MCB 2004
MICROBIOLOGY
TEST #1, FEBRUARY 4, 2009
IN THIS PART THERE ARE FORTY QUESTIONS WORTH TWO POINTS EACH. THERE
IS ONLY ONE CORRECT ANSWER FOR EACH QUESTION.
PLEASE CIRCLE THE
CORRECT LETTER OR PLACE THE CORRECT LETTER JUST TO THE LEFT OF THE
QUESTION NUMBER.
THEN CAREFULLY TRANSFER ALL YOUR ANSWERS TO THE
"ACUSCAN" ANSWER SHEET.
KEY FOR QUESTIONS 1-4. THERE IS ONLY ONE CORRECT ANSWER BUT EACH KEY
WORD MAY BE USED MORE THAN ONCE (OR NOT AT ALL).
A.
B.
C.
D.
E.
MITOCHONDRIA
ENDOPLASMIC RETICULUM
NUCLEI
FIMBRIAE (OR PILI)
RIBOSOMES
D_____1.
FOUND ONLY IN PROKARYOTES
E_____2.
COMPOSED OF THREE RNAs AND 50 PROTEINS
A____3.
HERE ATP IS PRODUCED BY OXIDATIVE PHOSPHORYLATION
E_____4.
FOUND IN BOTH PROKARYOTES AND EUKARYOTES
5.
IN THE ACID FAST STAIN THE PRIMARY STAIN IS _______, AND ACID-FAST
BACTERIA APPEAR __________ .
A.
METHYLENE BLUE
PINK (OR RED)
B.
IODINE
PURPLE
C.
CRYSTAL VIOLET
PURPLE
D.* CARBOL FUCHSIN
PINK (OR RED)
E.
MALACHITE GREEN
BLUE
6.
STAINING FIXED CELLS ONLY WITH CRYSTAL VIOLET IS AN EXAMPLE OF:
A.
SPECIAL STAINING
B.
DIFFERENTIAL STAINING
C.
BOTH OF THE ABOVE
D.* NEITHER OF THE ABOVE
7.
THE ACID FAST "OUTER MEMBRANE" IS UNUSUALLY WAXY BECAUSE OF THE
A.* MYCOLIC ACIDS
B.
PEPTIDOGLYCAN
C.
BOTH OF THE ABOVE
D.
NEITHER OF THE ABOVE
MCB 2004
TEST #1
PAGE 2
8.
IN BRIGHT FIELD MICROSCOPY LIGHT PASSING THROUGH THE
CONDENSER NEXT PASSES THROUGH THE
A.
OBJECTIVE LENS
B.
LIGHT SOURCE
C.
MAGNETIC FIELD
D.
OCULAR LENS
E.* THE SAMPLE (ON THE STAGE)
9.
THE INITIAL, SLOW PHASE OF BACTERIAL GROWTH IS CALLED THE
A.
STATIONARY PHASE
B.
LOG PHASE
C.* LAG PHASE
D.
DEATH PHASE
E.
PRE-REPLICATION PHASE
10.
THE MICROORGANISMS THAT ARE PATHOGENS FOR MAN ARE USUALLY
A.
ACIDOPHILES
B.
THERMOPHILES
C.
MICROAEROPHILES
D.* MESOPHILES
E.
PSYCHROPHILES
11.
THE DOUBLING TIME OF E. COLI IN NUTRIENT BROTH AT 37o C IS
40 MINUTES. THE INITIAL INOCULUM OF THE CULTURE CONTAINS 60
CELLS. AFTER TWO HOURS OF EXPONENTIAL GROWTH THE CULTURE
CONTAINS
A.
240 CELLS
B.
300 CELLS
C.* 480 CELLS
D.
600 CELLS
E. 1200 CELLS
12.
A BACTERIUM THAT OXIDIZES SULFUR WITH OXYGEN FOR ENERGY AND
USES CARBON DIOXIDE AS ITS CARBON SOURCE IS A
A.
CHEMOHETEROTROPH
B.
STRICT ANAEROBE
C.
BOTH OF THE ABOVE
D.* NEITHER OF THE ABOVE
13.
THE FOLLOWING IS ONE INGREDIENT OF A DEFINED (MINIMAL)
MEDIUM
A.
5% SHEEP BLOOD
B.
BEEF HEART INFUSION
C.
EGG YOLK
D.
YEAST EXTRACT
E.* GLUCOSE
MCB 2004
TEST #1
PAGE 3
14.
IN THE 17TH CENTURY HE VIEWED MICROORGANISMS THROUGH FINE LENSES
THAT HE GROUND HIMSELF.
A.
ROBERT KOCH
B.
JAMES WATSON
C.* ANTON VAN LEEUWENHOEK
D.
EDWARD JENNER
E.
LOUIS PASTEUR
15.
THE LIPOPOLYSACCHARIDE IS ANCHORED BY FATTY ACIDS (LIPID A) IN
THE
A.
PLASMA MEMBRANE
B.
PEPTIDOGLYCAN
C.* OUTER MEMBRANE
D.
MYCOLIC ACID LAYER
16.
IN 1800 50% OF MORTALITY IN THE USA WAS DUE TO INFECTIOUS
DISEASES; 25% DIED OF THIS ONE DISEASE
A.
PNEUMONIA
B.
CHOLERA
C
POLIO
D.* TUBERCULOSIS
17.
THE LIGHT MICROSCOPE
MAGNAFICATION.
A.
0.4 mm,
10
B.
10 m,
100
C.* 0.2 m,
2,000
D.
2 nm,
100,000
IS CAPABLE OF ____ RESOLUTION, AND ____
X
X
X
X
18.
ONE METER EQUALS
A.
100 mm
B.
1000 m
C.
BOTH OF THE ABOVE
D.* NEITHER OF THE ABOVE
19.
THE NUMBER OF CFU PER ML IN THIS CULTURE FLASK IS
A.
560
B.
5,600
C.
56,000
D.*
560,000
E. 5,600,000
1.0 ml
1.0 ml
9.0 ml
1.0 ml
9.0 ml
0.1 ml
9.0 ml
56 COLONIES
MCB 2004
TEST #1
PAGE 4
20.
THIS TYPE OF MICROSCOPE IS USED IN CLINICAL DIAGNOSES AND
DEPENDS UPON TAGGING BACTERIA WITH DYE-BOUND ANTIBODIES
A.
DARK FIELD MICROSCOPE
B.
BRIGHT FIELD MICROSCOPE
C.* FLUORESCENT MICROSCOPE
D.
PHASE CONTRAST MICROSCOPE
E.
ELECTRON MICROSCOPE
KEY FOR QUESTIONS 21-24. THERE IS ONLY ONE CORRECT ANSWER, BUT EACH
ANSWER MAY BE USED MORE THAN ONCE (OR NOT AT ALL).
A.
B.
C.
D.
E.
ESCHERICHIA COLI
BACILLUS SUBTILIS
MYCOBACTERIA LEPRAE
CHLAMYDIA TRACHOMATIS
BORRELIA BURGDOFERI
D_____21. ITS INTRACELLULAR, GROWING FORM IS CALLED A RETICULATE BODY
E_____22. IT MOVES USING ITS AXIAL FILAMENTS
C_____23. ARABINOGALACTAN ANCHORS ITS MYCOLIC ACIDS TO THE CELL WALL
B_____24. TEICHOIC ACIDS ARE PART OF ITS ENVELOPE
25.
KILLING THE UNDESIRABLE (PATHOGENIC) ORGANISMS USING MOIST
HEAT AT 63OC IS KNOWN AS
A.
FILTRATION
B.
INCINERATION
C.
STERILIZATION
D.* PASTEURIZATION
E.
LYOPHILIZATION
26.
MANY ANTIBIOTICS AND VITAMINS WHICH ARE UNSTABLE TO HEAT ARE
ADDED TO BACTERIAL GROWTH MEDIA AFTER THEY ARE
A.
AUTOCLAVED
B.
PASTEURIZED
C.
STEAMED
D.* FILTERED
E.
BOILED
27.
COMMERCIAL STERILIZATION OF LABORATORY PLASTIC-WARE IS USUALLY
ACCOMPLISHED BY
A.
RINSING IN ALCOHOL
B.
AUTOCLAVING
C.
TREATING WITH PHENOL
D.
SPRAYING WITH IODINE
E.* GASING WITH ETHYLENE OXIDE
MCB 2004
TEST #1
PAGE 5
28.
IN 1927 HE OBSERVED THAT THE GROWTH OF STAPHYLOCOCCUS AUREUS
WAS INHIBITED BY THE FUNGUS PENICILLIUM NOTATUM
A.
JONAS SALK
B.
LOUIS PASTEUR
C.* ALEXANDER FLEMING
D.
PAUL EHRLICH
E.
EDWARD JENNER
29.
PEPTIDOGLYCAN IS MADE OF ___________ POLYMERS
CROSSED-LINKED BY __________________ .
A.
PROTEIN
SUGARS
B.
LIPIDS
PEPTIDES
C.
CARBOHYDRATE
NUCLEOTIDES
D.* CARBOHYDRATE
PEPTIDES
E.
PROTEIN
LIPIDS
30.
STREPTOCOCCUS PYOGENES GROWS AS
A.
LONG THIN RODS
B.
CHAINS OF RODS
C.
CLUSTERS OF SPHERICAL CELLS
D.* CHAINS OF SPHERICAL CELLS
E.
PAIRS OF SPHERICAL CELLS
31.
A BACTERIAL FLAGELLUM IS MADE UP OF THREE PARTS. THEY INCLUDE
A.* THE HOOK
B.
THE MICROTUBULES
C.
BOTH OF THE ABOVE
D.
NEITHER OF THE ABOVE
32.
IN THE LACTOSE OPERON THE REPRESSOR BINDS TO THE
A.
THE PROMOTER
B.
THE lacZ GENE
C.
THE lacY GENE
D.
THE TERMINATOR
E.* THE OPERATOR
33.
THE OUTER PORTION OF THE LIPOPOLYSACCHARIDE (LPS) THAT CONSISTS
OF REPEATING THREE OR FOUR SUGAR UNITS IS
A.* THE O ANTIGEN (O SIDE CHAIN)
B.
THE CORE POLYSACCHARIDE
C.
MYCOLIC ACID
D.
LIPID A
E.
PHOSPHOLIPID
MCB 2004
TEST #1
PAGE 6
34.
THIS
A.
B.
C.*
D.
E.
COMPOUND IS A COMPONENT OF
DNA
RNA
PROTEIN
LIPID
CARBOHYDRATE
CH3
|
NH2-C-COOH
|
H
KEY FOR QUESTIONS 35-38. EACH QUESTION HAS ONLY ONE ANSWER, BUT EACH
ANSWER MAY BE USED MORE THAN ONCE (OR NOT AT ALL).
A.
B.
C.
D.
E.
THE KREBS (TCA) CYCLE
PROTEIN SYNTHESIS
ALCOHOLIC FERMENTATION
THE ELECTRON TRANSPORT CHAIN
GLYCOLYSIS
C_____35. THIS PROCESS PRODUCES 2 CO2s, TWO MOLECULES OF
ETHANOL AND TWO ATPs
A_____36. THREE NADHs, ONE FADH2, ONE ATP AND TWO CO2s ARE PRODUCED
FROM ONE ACETYL-CoA
B_____37. ANABOLISM OF A MACROMOLECULE
E_____38. TWO PYRUVIC ACIDS RESULT FROM ONE GLUCOSE
39.
DURING THIS PROCESS THE ANTICODON OF TRANSFER-RNA INTERACTS
WITH THE CODON ON THE MESSENGER-RNA.
A.
REPLICATION
B.
REVERSE TRANSCRIPTION
C.
TRANSCRIPTION
D.* TRANSLATION
E.
TRANSFORMATION
40.
THE ENZYME RNA POLYMERASE MAKES A STRAND OF RNA FROM A DOUBLESTRANDED TEMPLATE OF DNA; THIS IS CALLED
A.
REPLICATION
B.
REVERSE TRANSCRIPTION
C.* TRANSCRIPTION
D.
TRANSLATION
E.
TRANSFORMATION
MCB 2004
TEST #1
PAGE 7
41. FILL IN THE FOLLOWING
TABLE (1 POINT EACH, 10 POINTS)
BACTERIUM
MORPHOLOGICAL TYPE
DISTINCTIVE PROPERTY
OF CELL OR ENVELOPE
Bacillus subtilis
Escherichia coli
Mycobacterium leprae
thin peptidoglycan &
outer membrane
acid fast
Chlamydia trachomatis
Mycoplasma pneumoniae
Treponema pallidum
ANSWER ONE OF THE FOLLOWING TWO QUESTIONS (10 POINTS)
42.
43.
DRAW A TYPICAL BACTERIAL GROWTH CURVE AND LABEL IT (DON'T
FORGET TO LABEL THE AXES.).
OR
DESCRIBE THE STEPS (HINT: FOUR STEPS WITH WASHES IN BETWEEN)IN
THE GRAM STAIN. AT EACH STEP WHAT WOULD BE THE COLOR OF A FIXED
E. coli CELL.