3 A/P Biology Plant Organelles Pages 112-121 The plant cell has almost all the cellular inclusions that the animal cell has. In fact, the plant cell has some unique characteristics that define it as a plant cell. The plant cell differs from the animal cell by containing three structures NOT found in the animal cell- they are: 1.) The plant cell has a cell wall (in addition to a cell membrane or plasma membrane). 2.) The plant cell contains chloroplasts (these chloroplasts can contain special pigments that can convert light energy into chemical or food energy) Chloroplasts, like mitochondria, have their own DNA (circular DNA) and can replicate independently of the plant cell! 3.) The plant cell has a large water vacuole (containing mostly water). Below you will see a picture of a typical plant cell: If you compare the plant cell diagram to the animal cell diagram, you will note many similarities. Many of the cell inclusions are the same (they look the same and have the same function in each type of cell). One unusual difference between the plant cell and an animal cell is the disposition of centrioles. Animals cells have them, plants do not. They BOTH have a “centrosome” or microtubule organizing center. This is a generalized “area” where microtubules are produced to move chromosomes during mitosis and meiosis. Page 2 (Cont. A/P Biology Packet #3 I mentioned chloroplasts earlier, however different plants have other “plastids” that contain other pigments such as xanthenes and carotenes (that are typically found in chromoplasts) and some vacuoles contain starch material (amyloplasts) that are clear. I would like to first show a somewhat simplistic representation of how a mitochondria and then chloroplasts MAY have been incorporated into one of the earliest cells. (See below.) It is important to note that animal cells incorporated the mitochondria in the representation (as well as fungi and non-plant-like cells) and the plant cell evolved by taking in the mitochondria AND the chloroplasts (endosymbiosis, mutualism). Let us look at the three different organelles that make the plant cell different from the animal cell. I will start with arguably the most important difference. Chloroplasts The chloroplasts are actually very complex structures that usually contain Chlorophyll “A” and “B” that can absorb light. Below we see a picture of a typical chloroplast. It is important to note that BOTH mitochondria and chloroplasts contain their OWN DNA and can therefore replicate inside cells ON THEIR OWN! So- when energy requirements inside either the animal cell OR the plant cell change, mitochondria and chloroplasts can increase their numbers by simply dividing. The more chloroplasts available, the better Page 3 (Cont. A/P Biology Packet #3) the plant cell can make chemical energy from sunlight (through photosynthesis). By the same token, the more energy the plant OR animal cell needs in the form of Adenosine Triphosphate – abbreviated ATP- the energy “currency” of the animal or plant cell, the more mitochondria will be created (through simple division or fission). Mitochondria allow both animal AND plant cells to breakdown glucose efficiently. Chloroplasts allow plant cells to convert light energy into chemical energy (glucose). One important thing to think about is that not all plants/leaves are green. Some are red, some are yellow, and some are even brown in color. There are many different pigments found in plants that can absorb sunlight energy. The chloroplast is a specialized member of a family of related plant organelles called plastids. Amyloplasts can be colorless plastids that store starch (long chains of sugar molecules stuck together). Chromoplasts have pigments that give fruits and flowers their orange and yellow hues. Plastids that have green pigments called chlorophyll are the chloroplasts. The chloroplasts contain the green chlorophyll pigment and enzymes that help photosynthesis take place. (From: Campbell, N.A. and Reece, J. B. 2002. Biology. Benjamin Cummings. New York. p. 124) There are many different pigments that can help convert sunlight energy into chemical energy (food). Perhaps the most common are chlorophyll a and chlorophyll b. These pigments are found in ALL green plants and the pigments are expectedly, green. The chloroplast has two important functions: (1) absorb light (through a light dependent reaction)- this absorption of light taking place in the thylakoids (see diagram). (2) contains the Calvin Cycle (a series of enzymes complexes) that incorporates carbon dioxide molecules into sugar molecules- this part occurs in the stroma (see diagram- pg. 2 bottom rt.). Cell Wall Plant cell walls function primarily as rigid structures to give plant cells shape and resist turgor pressure. The cell wall is formed by repeating units (each unit is referred to as a monomer) of glucose called cellulose. (Absorbent cotton is one of the purest forms of cellulose.) The cellulose polymers (cellulose polymers are many glucose molecules stuck together) form microfibrils that are laid down randomly. (See picture to right.) Interspersed in the secondary cell wall matrix is a compound called lignin. This is what helps make fruits and vegetables crunchy and contributes to fiber in our diet. "Lignin is actually not one compound but many. All are Page 4 (Cont. Handout #3 AP Bio) complex, amorphous, three-dimensional polymers that have in common a phenyl propane structure, that is, a benzene ring with a tail of three carbons. In their natural unprocessed form, they are so complex that none of them has ever been completely described, and they have molecular weights that my reach 15,000 or more." (Taken from: McCrady, E. (1991). The Nature of Lignin. Avail: http://cool.conservationus.org/byorg/abbey/ap/ap04/ap04-4/ap04-402.html, pg. 1) Another view of 1o cell wall. Individual fibers look like this: You can imagine how many trillions of sugar molecules it takes to make a cell wall for a plant. To show the complexity and construction of these cell walls, go to: www.ceres.net then go to pull down menu: About us Biofuels Carbohydrates and cell walls Then “Click on the tabs marked: Cellulose, lignin, etc… Page 5 (Cont. #3 Handout A/P Biology) This primary cell wall is relatively thin and flexible. When the plant cell has stopped growing, a tougher, stronger cell wall is laid down just below the primary cell layer. This is called the secondary cell wall. (Located between the cell membrane and the primary cell wall.) The function of the cell wall is to allow the plant cell to resist water pressure (it is thought that the presence of lignin is responsible for this turgor pressure resistance) that can build up within the plant cell. The plant cell does not maintain an osmotic balance like the animal cell. Water moving into the plant cell (influx of water) can be tolerated because the cell walls Middle lamella are rigid and help prevent the made of Pectin-glue cell from bursting. It MUST be that holds plant cells noted that water is necessary for together photosynthesis to take placetherefore, the ability to store water is essential to the life of the plant! An internal hydrostatic pressure (hydrostatic pressure=water pressure) builds up within the cell, eventually equalizing the osmotic pressure and preventing further influx of water (this is called turgor pressure). The turgor pressure gives the plant cell shape, strength, and rigidity. Moreover, this turgor pressure prevents plants from wilting and gives specialized plant cells (xylem and phloem) strength enough to move water great distances from the roots to the leaves. Large water vacuole: The large water vacuole is primarily used to store water, food (especially sugar products), waste products, and salts. The water vacuole also helps give the plant cell shape and rigidity. Turgor pressure can cause the cell vacuole to elongate by taking in water, which is accumulated in the large central vacuole and makes the cell longer (and larger). See picture to the right. When the cell stops growing, the secondary cell walls are laid down and the cell Page 6 (Cont. Handout #3 AP Biology) becomes more rigid and stable. The movement of water through a plant depends on a balance between the water inside the plant cell and outside the plant cell. The movement of water throughout the plant (via xylem) is controlled by physical properties of water being attracted to each other (cohesion) and water attraction (interacting) with other surfaces (adhesion). Additional forces that help move water in the plant are osmotic pressure- the movement of water from an area of higher concentration to an area of lower concentration. The production of sugar is involved in this osmotic process! Answer 15 questions Here and on your scan tron- short answer on these sheets only! _____ 1.) Plant cells contain the following different organelles compared to the animal cells EXCEPT for this organelle (found in both cells): a.) chloroplasts b.) mitochondria c.) large water vacuole d.) cell wall e.) none of these _____ 2.) Plant cell contain all of the following structures EXCEPT: a.) cell wall b.) cell membrane c.) mitochondria d.) chloroplast e.) none of these _____ 3.) Mitochondria are beneficial to both animal cells and plant cells because they: a.) allow the cells to convert light energy to chemical energy b.) allow cells to efficiently breakdown sugar to form ATP c.) allow the cells to maintain turgor pressure d.) allow cells to perform osmosis e.) none of the above are correct _____ 4.) Chloroplasts: a.) are specialized plastids b.) contain light absorbing pigments c.) help the plant cell convert light energy to chemical energy d.) can divide on their own depending on demand for chemical energy e.) all of the above are correct _____ 5.) All light absorbing plant pigments are (Internet): a.) green b.) green or yellow c.) green, yellow, or red d.) green, yellow, red, or brown e.) plant pigments can be many different colors _____ 6.) Amyloplasts are structures that: a.) store amino acids to help build protein b.) store starch for energy reserves c.) store water, waste, and salts to create turgor pressure d.) store ATP for plant cell function e.) none of the above are correct Page 7 (Cont. #3Handout A/P Biology) _____ 7.) Chromoplasts are structures that: a.) store amino acids to help build protein b.) store different plant pigments c.) store waste, water, and salts to create turgor pressure d.) store ATP for plant cell function e.) store starch for energy reserves _____ 8.) Plastids that contain green pigments are called: a.) mitochondria b.) amyloplasts c.) myloblasts d.) centrioles e.) none of these _____ 9.) The location of the absorption of light in most plants is called: a.) amyloplasts b.) chlorakoids c.) neuroplast d.) angioplasty e.) thylakoids _____ 10.) Secondary cell walls are made up of: a.) protein and bi-lipid material b.) protein only c.) bi-lipid material only d.) repeating units of amino acids e.) repeating units of sugars and lignin _____ 11.) The primary cell wall is: a.) made of a very rigid cellulose structure b.) made up of a flexible cellulose structure c.) made up of proteins and bi-lipid membranes d.) made up of protein receptors e.) none of the above _____ 12.) The secondary cell wall is: a.) made up of repeating glucose units b.) more rigid than the primary cell wall (helps resist turgor pressure) c.) laid down between the cell membrane and the primary cell wall d.) produced only when the plant cell has stopped growing e.) all of the above are correct _____ 13.) What is the function of turgor pressure? a.) causes the cell wall to lose its rigidity b.) creates a negative osmotic gradient c.) slows down photosynthesis d.) moves water from an area of lower concentration to an area of higher concentration e.) gives plant cell shape, rigidity, and strength Page 8 (Cont #3 Handout A/P Biology) _____ 14.) What factor directly causes the plant cell to get larger? a.) increased photosynthesis b.) more mitochondria c.) water vacuoles losing water d.) plant cell nucleus transferring water to large water vacuole e.) increased turgor pressure within the large water vacuole _____ 15.) What causes a plant to wilt? a.) increased turgor pressure in the large water vacuole b.) addition of a secondary cell wall c.) subtraction of a primary cell wall d.) decreased photosynthesis e.) decreased turgor pressure Answer the following questions on this handout only! 16.) Explain why it was advantageous for Ancient Prokaryotes to incorporate mitochondria into their cells. Also, explain why some ancient prokaryotes incorporated chloroplasts as well as mitochondria. Speculate the effect of an ancient prokaryote only incorporating chloroplasts and NOT mitochondria into the cell. 17.) Where would chlorophyll a and chlorophyll b most likely to be found? If a plastid was green, what type of plastid would it be? If the plastid contained red and yellow pigments, what would it be called? In the Fall- green leaves turn to different colors. Based on the information you have already (and any you can garner from the Internet), why do leaves turn other colors? Also, during the time they are not green, do they still perform photosynthesis? 18.) If you are given the information that plant and plant-like organisms are the MAJOR sources of oxygen (due to the function of chloroplasts performing photosynthesis), speculate what might have happened if chloroplasts were never incorporated into prokaryotic cells (I am looking for global effects such as air quality, temperature, animal life, plant life, etc.. Hint: this loss of chloroplasts moving into prokaryotic cells would prevent the evolution of plants as we know it…) Date: ____________________ Lesson Plan for Handout #3 AP Biology Objective: TLWD ability to name all the cell components found in plant cells, determine which inclusions are found in plant cells and not in animals cells and determine the function of all plant cell inclusions, know what they look like and explain why plant cells are different from animal cells when given handout #3. Content: Plant cell organelles and function, variation of cell inclusion from animal cells Method: Handout #3 Homework: Complete #3 Comment: