Biol 205 Tentative Lab Schedule for 2007
Lab
Dates
Subject to Change
Classical Genetics
Flies
Jan 8 11
Other activities
Due At
Start of
Lab
NO LAB
Meiosis and Mitosis (15-18)
1
Jan 15 18
Cytogenetics (19-20)
karyotyping
Monofactorial crosses (21-22)
Getting to know your flies (43-51)
Probability theory (23 - 25)
2
Jan 22 25
Pedigree analysis (27 - 28)
*
before
next lab
3
Jan 29 Feb 1
Dihybrid crosses (26)
4
Feb 5 8
Sex Determination & Sex
Linkage (29-30)
learn how to
− handle flies
− identify ♂ and ♀
− recognize mutants
collect 5 ♀ virgins (procedure pg 53)
Set up parental cross (52-62)
1st
assignment
Use 4-5 of your virgin ♀ and 4-5 ♂ from your
partner's vile
Remove parents from cross
test
1 hr
Set up F1 cross
5
Feb 12 15
Genetic interactions (33-35)
Select 4 random ♂ and ♀ F1 flies and place
them in a new food vial. Make sure to
clearly label this vial.
Begin scoring F1 progeny
You need to score at least 50 F1 flies; if you
do not have enough today you will need to
come in later this week for more.
*
Before Mid Term Break
Remove parents from F1 cross
Applicable lab manual page numbers are indicated in parentheses.
Please read these pages before coming to lab.
2nd
assignment
Biol 205 Tentative Lab Schedule for 2007
Lab
Dates
Subject to Change
Classical Genetics
Feb 19 23
Flies
Other activities
Due At
Start of
Lab
MID TERM BREAK
Analysis of F1 results.
6
7
Feb 26 Mar 1
Evaluating genetic hypotheses,
2
χ testing (36-38)
Mar 5 8
linkage and chromosomal
mapping (33-35)
Count F2 flies.
You need score at least 100 F2 flies, if you
do not have this many today you will need
to come in later this week to score more.
Analysis of F2 data.
I must have all your data before this
week's lab!!!
3rd
assignment
Counting of test cross, analysis of data.
8
Mar 12 15
9
Mar 19 22
10
Mar 26 29
11
Apr 2 - 5
tetrad analysis
(this may change)
12
Apr 9 12
comprehensive lab exam
Drosophila report due
population genetics (39-40)
Analysis of PV92 loci in
humans. Extract DNA and set
up PCR rx. (82-90)
Analysis of PV92 loci in
humans. Separate PCR
products on agarose gel. (9197)
Computer Lab - analysis of
PCR results (98)
Applicable lab manual page numbers are indicated in parentheses.
Please read these pages before coming to lab.
Report Due
Analysis of Sordaria cross (6581)
4th
assignment