Name: Phone: PI: Date: Dept: Account #: Building and Room: UMASS Medical School Tissue Culture and Enzyme Freezer Supply - TCEFS Description Markers and Standards (freezer) Novex® Sharp Pre-Stained Standard Benchmark PreStain Protein Ladder MagicMark Western Protein Std SeeBlue Plus2 Pre-Stain Std ONE KB LADDER ONE KB PLUS LADDER 100 BP DNA Ladder Track-IT 100 BP DNA LADDER new Track-IT 1KB Plus DNA LADDER new Electrophoresis Size Cat # 2 x 250 µl 2 x 250 µl 250 µl 500 µl 250 µg 250 µg 50 µg 100 lanes 100 lanes LC5800 10748010 LC5602 LC5925 15615016 10787018 15628019 10488058 10488085 $132.00 $137.00 $248.00 $132.00 $153.00 $144.00 $96.00 $110.00 $121.00 Agarose UltraPure 100 g 15510019 Salmon Sprem DNA UltraPure 5 x 1 ml 15632011 SYBR Safe DNA Gel Stain 10,000X new 400 µl S33102 NuPAGE Novex Precast Gels (other types also available) $132.00 $121.00 $48.90 Nupage 4-12% BT Gel 1.5MM 10W 10 gels NP0335BOX Nupage 4-12% BT Gel 1.0MM 12W 10 gels NP0322BOX Competent Cells + TOPO TA Cloning Kits (-80°C) MAX EFF DH5α 5 x 200 µl 18258012 MAX EFF STBL2 5 x 200 µl 10268019 SBCLNG EFF DH5α 4 x 0.5 ml 18265017 TOP10 ONE SHOT 4 x 0.5 ml C404010 TOPO TA Cloning Kit With pCR2.1 20 rxn K450001SC Transfection Reagents (refrigerator) LIPOFECTAMINE RNAi MAX 1.5 ml 13778150 LIPOFECTAMINE 2000 1.5 ml 11668019 LIPOFECTAMINE 1 ml 18324012 LIPOFECTAMINE 4 x 1 ml 18324020 PLUS Reagent 0.85 ml 11514015 OLIGOFECTAMINE 1 ml 12252011 CELLFECTIN Reagent 1 ml 10362010 RNA Purification $127.00 $127.00 Trizol Reagent (Fridge) Trizol Reagent (Fridge) DYNAL Protein Purification (IP's) Dynabeads Protein A Dynabeads Protein G Antibodies Anti V5 Antibody Microscopy Prolong Gold Antifade Prolong Gold Antifade W/ PaP Selection Agents Geneticin (G418) Geneticin (G418) IPTG X-GAL Zeocin S.O.C. Medium (RT) $145.00 $256.00 100 ml 15596026 200 ml 15596018 Qty List $179.00 $223.00 $72.25 $208.00 $458.00 $522.00 $457.00 $292.00 $934.00 $156.00 $293.00 $207.00 5 ml 5 ml 10002D 10004D $555.00 $555.00 50 µl R96025 $200.00 10 ml 5 x 2 ml P36930 P36935 $123.00 $145.00 11811031 10131035 15529019 15520018 R25001 15544034 $313.00 $119.00 $53.00 $210.00 $202.00 $80.75 5g 20 ml 1g 1g 1g 10 x 10 ml Question? Call or email Account Manager David Spezzano 617-640-7579 david.spezzano@invitrogen.com Special orders must be emailed to enzymefreezer@umassmed.edu before 1pm (for next day delivery) TCEFS Description Platinum TAQ Polymerase (freezer) $132* PLATINUM PCR SUPERMIX $120 PLATINUM Taq DNA POL $248 PLATINUM Taq DNA POL $132 PLATINUM Pfx POLYMERASE $153 PLATINUM Pfx POLYMERASE $144 PLATINUM Taq HIGH FID. $96 PLATINUM Taq HIGH FID. $110 TAQ Polymerase (freezer) Size Cat# Qty List TCEFS 100 rxn 100 rxn 500 rxn 100 rxn 500 rxn 100 rxn 500 rxn 11306016 10966018 10966034 11708013 11708039 11304011 11304029 $137.00 $95.00 $401.00 $120.00 $512.00 $153.00 $686.00 $110 $79 $334 $113 $483 $136 $609 $121 PCR SUPERMIX 100 rxn Taq DNA POLYMERASE, rec 100 u $132* Taq DNA POLYMERASE, rec 500 u $121 BSA and Modifying Enzymes (freezer) $49* Bovine Serum Albumin 150 mg Dnase I AMP Grade 1unit/ul 100 u $127 Proteinase-K 100 mg $127 Proteinase-K, RNA Grade, 20 mg/ml 5 ml Ribonuclease H 2units/ul 4 x 30 u $179 RNaseOUT Recomb. 5,000 u $203 T4 DNA LIGASE 100 u $60 RT Enzymes (freezer) 10572014 10342053 10342020 $103.00 $59.00 $269.00 $66 $36 $110 15561020 18068015 25530015 25530049 18021071 10777019 15224017 $178.00 $104.00 $96.00 $115.00 $383.00 $127.00 $56.75 $178 $100 $68 $111 $361 $96 $49 $208 SUPERSCRIPT II 10,000 u 18064014 $260.00 $458 SUPERSCRIPT III 10,000 u 18080044 $255.00 SUPERSCRIPT 1st Strand Sys 50 rxn 11904018 $352.00 $522* OLIGO(DT)12-18 PRIMER 25 µg 18418012 $93.75 $436 Nucleotides (freezer) $292 100 mM dNTP SET 4 x 25 µM 10297018 $240.00 $881 10 mM dNTP MIX 100 µl 18427013 $74.25 $156* Plasmid Purification Kits $280 PureLink™ Quick Plasmid Miniprep Kit 50 preps K210010 $70.75 $202 PureLink™ Quick Plasmid Miniprep Kit 250 preps K210011 $315.00 PureLink™ HiPure Plasmid Miniprep Kit 25 preps K210002 $164.00 $114 PureLink™ HiPure Plasmid Miniprep Kit 100 preps K210003 $532.00 $224 PureLink™ HiPure Plasmid Midiprep Kit 25 preps K210004 $217.00 PureLink™ HiPure Plasmid Midiprep Kit 50 preps K210005 $392.00 $555* PureLink™ HiPure Plasmid Maxiprep Kit 10 preps K210006 $188.00 $555* PureLink™ HiPure Plasmid Maxiprep Kit 25 preps K210007 $434.00 PureLink™ HiPure Filter Midiprep Kit 25 preps K210014 $250.00 $200* PureLink™ HiPure Filter Maxiprep Kit 10 preps K210016 $207.00 DNA Clean-up and Gel Extraction $123* PureLink™ PCR Purification Kit 50 preps K310001 $87.50 $145* PureLink™ Gel Extraction Kit 50 preps K210012 $87.50 Special Orders Molecular Biology Essentials $219 $96 $52 $207 $164 $80 FREE SHIPPING! *Minimally discounted item, $5-30 handling fee applies (you still save with free shipping) Prices effective 1-1-10 to 12-31-10 $248 $241 $332 $89 $178 $48 $64 $287 $149 $478 $202 $366 $175 $385 $228 $195 $80 $80