Personal Care
Consumer Care
Biovert™ Enzyme & Substrate (patented)
A Two-Part Natural Protection System
Biovert™ Enzyme
INCI Name: Glucose Oxidase & Lactoperoxidase
SAP Code#: 135940
Biovert™ Substrate
INCI Name: Glucose
SAP Code#: 135930
Key Product Benefits
–– Safe, effective, natural
–– Anti-Microbial
–– Anti-oxidant
Recommended Use Levels
Enzyme solution at 0.05% by weight
Substrate solution should be dosed at 1.0% by weight
Fig. 1
Mode of Action
Biovert™ mimics a naturally occurring antimicrobial-antioxidant protection
system. When the two-part system is combined, a cascade of linked
reactions takes place to generate antimicrobial products in situ. The
cascade is initiated by the action of the glucose oxidase enzyme in the
presence of its substrate (glucose) and oxygen. This generates H2O2,
which is used by the lactoperoxidase to catalyze the oxidation of I- and SCNanions, forming hypoiodite and hypothiocyanate which have antimicrobial
activity. The result is rapid microbial cell death expression.
Formulation Recommendations
–– Prepare base formula with all ingredients but
Biovert™ Enzyme & Substrate
–– Adjust pH to 4 - 6; Cool <40°C
–– Add Substrate to give 1.0% final concentration
–– Add Enzyme to give 0.05% final concentration
–– Mix thoroughly to ensure complete blending
–– IMPORTANT – add substrate and enzyme separately to formula
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Temperature Information
Efficacy - CTFA Challenge Test
Microbiology Challenge Study
–– Processing temperatures should be <40°C
–– Emulsion containing Biovert™ System – stability shown†:
–– 40°C – more than 2 months
–– 37°C – 3 months
–– 35°C – 6 months
–– 25°C – more than 2 years
–– Every formula needs to be challenge tested under the user’s
stability protocols
It is well established that Biovert™ has broad spectrum antimicrobial effects in a wide range of formulation types. Data from recent evaluations
demonstrating performance of Biovert™ in a water in oil emulsion containing silicone is shown below. A CTFA challenge protocol was followed.
All samples were diluted in neutralizer and plated quantitatively for viable organisms after 28 days. After 28 days, all samples were subjected
to a second challenge.
The results show that the formulation containing Biovert™ was able to
withstand repeated microbial challenges [bacteria, yeast and mold]
while the unpreserved controls were susceptible to contamination.
† = according to BP challenge testing
The Effect of Temperature on the Antimicrobial Efficacy
of Biovert™ in an Oil in Water Emulsion
Temperature
Organism
55C
50C
45C
Control
Test Biovert™
24 Hours
28 day CTFA
Re-challenge
24 Hours
4 Week
Challenge
P. aeruginosa
4.4 x 104
1.0 x 106
<10
<10
K. pneumoniae
6.0 x 104
1.9 x 104
<10
<10
S. aureus
8.0 x 105
1.1 x 106
<10
<10
E. gergoviae
(product isolate)
1.1 x 10
2.4 x 10
4
<10
<10
C. albicans
3.6 x 103
1.0 x 103
<10
<10
A. niger & Penicillium sp.
2.0 x 10
3.6 x 103
<10
<10
4
3
40C
USA
Lonza Consumer Care
70 Tyler Place
South Plainfield, NJ 07080
Tel +1 908 561 5200
35C
30C
25C
0
2
4
6
8
10
12
14
16
18
20
22 24
Time (weeks)
Bacterial Pass - BP Challenge Test
Fungal Pass - BP Challenge Test
This product information corresponds to our knowledge on the subject at the date of publication and
we assume no obligation to update it. It is offered without warranty, and is intended for use by persons who are experienced and knowledgeable in the field and capable of determining the suitability
of ingredients for their specific applications. Because we cannot anticipate all variations in actual enduse conditions, we assume no liability and make no warranty in connection with your use of our products or product information. We do not guarantee the efficacy of active ingredients, delivery systems,
functional ingredients, rheology modifiers, natural or botanical products, preservative and protection
systems or proteins in any specific application or use. The information we provide is not intended to
substitute for testing. You should perform your own tests to determine for yourself the suitability and
efficacy of ingredients in your application and conditions of use. The information we provide should
not be construed as a license to operate under or a recommendation to infringe any patent or other
intellectual property right, and you should ensure that your use does not infringe any such rights. Our
products are for industrial use only. WE MAKE NO WARRANTY (INCLUDING AS TO MERCHANTABILITY OR
FITNESS FOR PURPOSE) OF ANY KIND, EXPRESS OR IMPLIED, OTHER THAN THAT OUR PRODUCTS CONFORM
TO THE APPLICABLE PRODUCT SPECIFICATIONS.
© 2014 Lonza Ltd
Personal Care – Biovert™ Enzyme & Substrate – 12/14
Switzerland
Lonza Ltd
Muenchensteinerstrasse 38
4002 Basel
Tel +41 61 316 81 11