Chapter 14 Summary (Electron transport and oxidative phophorylation)
1) Know the chemosmotic theory
2) Know the names of the major components of the mitochondria and the functions
and properties of these components.
3) Understand how reduction potentials of re-dox pairs relate to electron transport.
4) Know that electrons can only be transferred to electron acceptors with less
negative or more positive reduction potentials. If given a number of electron
carriers with their reduction potential, be able to order them in a hypothetical
electron transport chain.
5) Know the general properties of each of the mitochondrial electron transfer chain
complexes (I through IV).Know the order of these complexes in the electron
transport chain.
6) Know what are the mobile lipid soluble and water soluble electron carriers. Know
the general properties of these carriers.
7) Know at which points in the mitochondrial electron transport chain are protons
pumped into the matrix.
8) Understand the role of the Q cycle (it is required to transfer electrons from a 2
electron donor to a one electron acceptor).
9) Know what the initial electron donor and the terminal electron acceptor is for the
mitochondrial electron transport chain.
10) Know where the inhibitor, rotenone, acts in the mitochondrial electron transport
chain.
11) Know how electrons (electron equivalents) generated in the cytoplasm are shuttle
into the mitochondria and donated to the electron transport chain. Know the
glycerol phosphate shuttle and the malate/aspartate shuttle. Know approximately
how many ATPs will be formed from electrons donated from either shuttle and
why.
12) Understand how chemical uncouples of the mitochondrial ETC and oxidative
Phosphorylation work. Know what happens to the energy generated through the
ETC when uncoupling occurs.
13) Know the biological examples of uncoupling and their physiological role in
animals and plants.
14) Proton motive force. Know that PMF is derived from a charge potential and pH
potential generated across the mitochondrial membrane. Know which contributes
more to the energy generated through PMF.
15) Know the general structure of the F1 Fo ATPase. Know what activities are
associated with the F1 and Fo domains. Know where each domain is located in
the mitochondrial.
16) Know the binding change mechanism of ATP formation.
17) Know the properties of the ADP/ATP translocator and the Pi/H+ symport.
Understand how the transport of these compounds across the inner envelope
relates to PMF and energy consumption.
18) Know how ETC and oxidative Phosphorylation are regulated by ADP and ATP
levels.