CLASS: M.Sc. BIOTECHNOLOGY
11N/ 311
St. JOSEPH’S COLLEGE (AUTONOMOUS) TIRUCHIRAPPALLI – 620 002
SEMESTER EXAMINATIONS – NOVEMBER 2011
TIME: 3 Hrs.
MAXIMUM MARKS: 100
SEM
SET
PAPER CODE
TITLE OF THE PAPER
III
2010
10PBT3110
ANIMAL BIOTECHNOLOGY
SECTION – A
Answer all the questions:
20 x 1 = 20
Choose the correct answer:
1.
2.
3.
4.
5.
The fusion of different types of animal cells are termed as _______
a)
Protoplast fusion
b)
Cybrid
c)
Somatic cell fusion
d)
All the above
Mabs refers to _______
a)
Multiple antibodies
b)
Multiple antibiotics
c)
Mode of action
d)
Monoclonal antibody
The most commonly used baculovirus is _______
a)
ACMNPV
b)
MCP
c)
AMN
d)
Adenovirus
The technology implemented for the production of transgenic animals is _______
a)
Invitro technology
b)
Nuclear transfer technology
c)
Protoplast fusion technology
d)
Cybrid technology
A technique for the identification of individuals based on the small difference in DNA sequence
is _______
a)
DNA finger printing
b)
DNA marker
c)
DNA micro array
d)
DNA sequence finding
Fill in the blanks:
6.
Cell line is that animal cells can be cultivated under _______ condition.
7.
Pharm animals mean transgenic animal used to produce _______ product.
8.
The use of entomopathogenic fungi for the control of insect pest is _______ pesticides.
9.
Any one example for chemical cryoprotectant is _______.
10.
Kary Mullis (1985) developed the technique _______.
State True or False:
11.
DMEM means Dulbecco’s modified minimal essential medium.
12.
Genetic engineering is used for development of pathogenic animals.
13.
The advantages of vaccinia expressions, elicit humoral and cellular immune response.
14.
Androgenesis is a process to obtain all male progency.
15.
Nucleic acid amplification was performed by western blotting technique.
Answer in one or two sentences:
16.
Established cell line.
17.
Viral vectors.
18.
Pest management by Biotechnological approach.
19.
IVF.
20.
Intergenic DNA.
SECTION – B
Answer all the questions:
21.
a.
5 x 4 = 20
Mention the Disaggregation of animal tissue.
OR
22.
b.
Write the importance of animal cell culture in biomedical research.
a.
Narrate on expression vector and its importance.
OR
23.
b.
Write short notes on somatic cell hybridization.
a.
Write a brief account on transgenic fishes.
OR
24.
b.
Write the role of polyheabuin gene of Baculovirus.
a.
Describe the gynogenesis process and its objective in brief.
OR
25.
b.
Write the role of Biotechnology in aquaculture.
a.
Write the application of DNA finger printing.
OR
b.
Describe polymerase chain reaction (PCR) in brief.
SECTION – C
Answer any FOUR questions:
4 x 15 = 60
26.
Essay on various types of culture media used in Animal cell culture.
27.
Describe the mechanism and achievements in the production of Transgenic animal.
28.
Write the strategies involved in the production of growth hormone using Transgenic fishes.
29.
Describe the methods involved in cryobiology and add a mote on its role.
30.
Write an essay on various methods of gene transfer.
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