From: jafc@internode.on.net
To: chenqx@xmu.edu.cn
CC: jafc@internode.on.net
BCC:
Subject: Revision Requested for Manuscript ID jf-2009-007554
Body: 27-Apr-2009
Journal: Journal of Agricultural and Food Chemistry
Manuscript ID: jf-2009-007554
Title: "Anti-tyrosinase and Antimicrobial Activities of trans-Cinnamaldehyde
Thiosemicarbazone"
Author(s): Zhu, Yu-Jing; Song, Kang-Kang; Li, Zhi-Cong; Pan, Zhi-Zhen; Zhou,
Jing-Jing; Wang, Qin; Liu, Bo; Chen, Qing Xi
Dear Prof. Dr. Chen:
Your manuscript was reviewed carefully by myself and referred to reviewers for
comment. On the basis of this study, I have decided to accept the manuscript for
publication pending minor revisions incorporating the reviewers comments. Reviewer
comments appear at the end of this letter.
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Table of Contents categories: Analytical Methods; Bioactive Constituents; Biofuels and
Bioproducts Chemistry; Chemical Aspects of Biotechnology/Molecular Biology;
Chemical Aspects of Food Safety; Chemical Changes Induced by Processing/Storage;
Chemical Composition of Foods/Feeds; Crop and Animal Protection Chemistry;
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Chemistry/Biochemistry; Molecular Nutrition; Toxicology in Agriculture and Food
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Reviewer: 1
Recommendation: Publish after minor revisions.
Comments:
Tyrosinase inhibitors have been established as important constituents of
depigmentation agents and have potential uses as food preservatives. For the article,
authors first synthesized trans-cinnamaldehyde thiosemicarbazone, then investigated
its bioactivities of the inhibitory mechanism on tyrosinase and its antibacterial effects.
trans-Cinnamaldehyde thiosemicarbazone was a potent tyrosinase inhibitor and was of
potent effect against B. subtilis and S. aureus, which may be a kind of food
preservatives and insecticides. All these kinetic data may provide the basis for
developing novel tyrosinase inhibitors and developing new potent food preservatives.
The paper is well-written and I thank this article is of great practical and theoretical
significance with high quality. It should be accepted for publication in Journal of
Agricultural and Food Chemistry after minor revisions:
1. The unit “mL” should be “ml”.
2. Page 4, line 15, the sentence “Recently, benzaldehyde thiosemicarbazone derivatives
have been the inhibitors of PO from insects (8).” It should be changed to “Recently,
benzaldehyde thiosemicarbazone derivatives have been discoverd as the inhibitors of
PO from insects (8).”
3. Page 4, line 19, the word “pantitumor” in the sentence should be “antitumor”.
4. What is the IC50 value of trans-cinnamaldehyde thiosemicarbazone on
monophenolase activity of tyrosinase?
5. Page 12, line 3, according to table II, The MBC of trans-cinnamaldehyde
thiosemicarbazone against E.Coli should be 100 ug/ml, but not 50 ug/ml.
6. What is the possible reason for the bacteriostatic activity of trans-cinnamaldehyde
thiosemicarbazone?
Originality: Good
Technical Quality: Good
Clarity of Presentation: Good
Importance to Field: Good
Reviewer: 2
Recommendation: Publish after minor revisions.
Comments:
Tyrosinase (1.14.18.1) catalyzes two distinct reactions of melanin synthesis---the
hydroxylation of monophenol and the oxidation of o-diphenol to the corresponding
o-quinone. It is a key enzyme for melanin biosynthesis in plants and animals. It is
important and valuable to search compounds which possess both anti-tyrosinase and
anti-microbial abilities as it could be potentially used in food protection. The authors of
this manuscript synthesized trans-cinnamaldehyde thiosemicarbazone and found it
exert ed potent inhibition on the activities of monophenolase and diphenolase of
tyrosinase. The inhibition mechanism was also well studied. The experimental results
will provide an informative data. The finding would be important for developing novel
tyrosinase inhibitors and developing new potent food preservatives. Thus, this
manuscript is valuable for advancing scientific knowledge in the field. It is
recommended for publication after the following amendment:
1. There are some spelling mistakes and solecism in this paper. For example, all the unit
“mL” should be “ml”; Page 3, line 46, the word “pantitumor” in the sentence should be
“antitumor”; Page 8, line 8-16, there is a lack of the definite article in these sentences;
Please check them through the manuscript.
2. Some notes should be made concerning the kinetic equations. For example, the
equation (1) contains v0. There is no v0 in the list of designations.
3. Page 10, line 32-35, this sentence is unclear.
Originality: Excellent
Technical Quality: Good
Clarity of Presentation: Good
Importance to Field: Excellent
Reviewer: 3
Recommendation: Publish without change.
Comments:
I've reviewed this paper carefully and the manuscript would be acceptabe at this
formation.
Originality: Good
Technical Quality: Good
Clarity of Presentation: Excellent
Importance to Field: Good
Date
Sent:
27-Apr-2009