7th Grade Curriculum Unit 1: Cells Unit 2: Genetics PA Academic Standards 3.1.7.A1: Describe the similarities and differences of major physical characteristics in plants, animals, fungi, protists, and bacteria. 3.1.7.A4: Explain how cells arise from pre-esixting cells. 3.1.7.A5: Explain how the cell is the basic structural and functional unit of living things. 3.1.7.A6: Identify the levels of organization from cell to organism. 3.1.7.A7: Compare life processes at the organism level with life processes at the cellular level. 3.1.7.A8: MODELS: Apply the appropriate models to show interactions among organisms in an environment. 3.1.7.A9: * Understand how theories are developed. * Identify questions that can be answered through scientific investigations and evaluate the appropriateness of questions. * Design and conduct a scientific investigation and understand that current scientific knowledge guides scientific investigations. * Describe relationships using inference and prediction. * Use appropriate tools and technologies to gather, analyze, and interpret data and understand that it enhances accuracy and allows scientists to analyze and quantify results of investigations. * Develop descriptions, explanations, and models using evidence and understand that these emphasize evidence, have logically consistent arguments, and are based on scientific principles, models, and theories. * Analyze alternative explanations and understanding that science advances through legitimate skepticism. * Use mathematics in all aspects of scientific inquiry. * Understand that scientific investigations may result in new ideas for study, new methods, or procedures for an investigation or new technologies to improve data collection. 3.1.7.B1: Explain how genetic instructions influence inherited traits. Identify Mendelian patterns of inheritance. 3.1.7.B2: Compare sexual reproduction with asexual reproduction. 3.1.7.B4: Describe how selective breeding and biotechnology can alter the genetic composition of organisms. 3.1.7.C2: Explain that mutations can alter a gene and are the original source of new variations in a population. 1 Classroom Expectations 1. Be prepared for class every day. You will need a pencil, folder, workbook, and agenda book every day. Pens are optional for everything except lab work and tests! 2. Be on time. If you arrive after the bell rings, be prepared to go to the office for a late pass. 3. Be respectful of your classmates, your teacher and your self. 4. When I am speaking, YOU are NOT! 5. Your full attendance is required. By this I mean you must be both mentally and physically present in class. Participating in discussions, and volunteering will add to your educational experience, as well as keeping class interesting for you! 6. IF you are absent it is YOUR responsibility to make up all missed work. Please see me at the beginning of class as soon as you return, so I can get you any information you missed. All missing work is recorded as a 0% until it is made up. Then full credit will be given if assignments are turned in by the appropriate deadline given. 7. Lab work is REQUIRED. If you are absent on a lab day, it is your responsibility to schedule a make-up lab during period 7. 8. Passes for band lessons will not be accepted on test or lab days. 9. If you have any questions or concerns please see Mrs. Bicher as soon as possible. I don’t want you to get behind. I am happy to help, but YOU need to let me know that you need help! 10. Please check PowerSchool, and Blackboard from home to keep on top of homework assignments and grades! This is a great tool that will help you to succeed in my class. GRADE SCALE A+ A AB+ B B- 98% - 100% 93% - 97% 90% - 92% 87% - 89% 83% - 86% 80% - 82% C+ C CD+ D D- 77% - 79% 73% - 76% 70% - 72% 67% - 69% 63% - 66% 60% - 62% F 0% - 59% Please read through the above information with your parents. If you have any questions or concerns you can reach me best through e-mail. abicher@elcosd.org Please sign the appropriate line below, and have your parents sign as well to show me you read and understand the classroom expectations for 7th grade biology. ____________________________________ ____________________________________ Student signature parent signature 2 Cells What is a cell? _______________________________________________________ Anton VanLeeuwenhoek: _______________________________________________ Robert Hooke _______________________________________________________ Matthias Schleiden, Theodore Schwann and Rudolf Virchow wrote the cell theory: 1. _______________________________________________________________ 2. _______________________________________________________________ 3. ________________________________________________________________ What is a scientific theory? __________________________________________________________________ What is spontaneous generation? __________________________________________________________________ __________________________________________________________________ How did Francis Redi’s experiment disprove the theory of spontaneous generation? __________________________________________________________________ __________________________________________________________________ Cell types: Red blood cells _____________________________________________________ White blood cells ___________________________________________________ Nerve cells ________________________________________________________ Muscle cells _______________________________________________________ Bone cells __________________________________________________________ Skin cells ___________________________________________________________ Stem cells __________________________________________________________ 3 All About Me Lab In this lab you will observe several different cell types. You will see nerve cells, red blood cells, muscle cells, etc… As you observe each slide, you should draw what you see in the circles below. On the line below each circle you should write in the magnification used (ie. 10X, 40X…). On the line to the right of the circle is the name of the slide you should be drawing in that circle!! Human Smooth Muscle, Uterus _______________________ Human Blood Smear ________________________ 4 Mammal Compact Bone Ground ______________________ Human skin Nonpigmented _____________________ Nerve cell _______________________ You may continue to look at any of the slides in the grey box until the bell rings. You don’t have to draw any more, but look at some of the cool cells from a human body! 5 Conclusion: Answer the following questions in complete sentences. SPELLING COUNTS! 1. List the 5 cell types you looked at and describe their function in the human body. _______________________________________________________________________ _______________________________________________________________________ _______________________________________________________________________ _______________________________________________________________________ _______________________________________________________________________ _______________________________________________________________________ 2. What is the advantage to having so many different cell types? Wouldn’t it be easier to be all one cell type? _______________________________________________________________________ _______________________________________________________________________ _______________________________________________________________________ _______________________________________________________________________ _______________________________________________________________________ 3. Explain what the cell theory is and why it is an important advancement in biology. How did the advancement in microscopes help with the cell theory and other advancements in biology? _______________________________________________________________________ _______________________________________________________________________ _______________________________________________________________________ _______________________________________________________________________ _______________________________________________________________________ 4. Describe the processes needed to use a microscope. Go through the steps from where you put the slide to seeing the final object. For example: Step 1 plug in the microscope and turn the light on. Step 2….. _______________________________________________________________________ _______________________________________________________________________ _______________________________________________________________________ _______________________________________________________________________ _______________________________________________________________________ _______________________________________________________________________ _______________________________________________________________________ 6 Organization of Life from smallest to largest: __________ ____________ ____________ ____________ ____________ Earthworm Anatomy Lab notes 7 External Anatomy: ___________________________ The underside (belly) of the worm. ___________________________ The top side of the worm. ___________________________ The “head end” of the worm. This is the shortest portion of the earthworm. From the clitellum to the head (front) of the worm. ___________________________ The “tail end” of the worm. This is the longest portion of the earthworm. From the clitellum to the end of the worm. ___________________________ Secretes a cocoon around the fertilized eggs. ___________________________ Bristle like structures that help the worm to move and act to sense the environment. ___________________________ The skin or outer layer of the worm. It secretes mucous. The earthworm has no gills or lungs. Gases are exchanged between the circulatory system and the environment through the moist skin. ____________________________Are found in pairs in each body segment. They appear as tiny white fibers on the dorsal wall. Excretory functions are carried on here. On the drawing below label the anterior, posterior, dorsal and ventral sides. Also label the clitellum. Internal Anatomy: _________________________ are little thread like structures that hold the skin to the organs below. _________________________ or body cavity where the various organs, and systems are held. Reproductive System: Earthworms are _____________________________ because they have both male and female reproductive organs. The _______________________ is a swelling of the body found in sexually mature worms and is active in the formation of an egg capsule, or cocoon. Eggs are produced in the ____________________ (found on segment 13, and pass out of the body through female genital pores. Sperm are produced in the ______________________ (found between segment 10 and 11), and pass out through tiny male genital pores. During mating, sperm from one worm travel along the sperm grooves to the _______________________ of another worm. Fertilization of the eggs takes place __________________________ the body as the cocoon moves forward over the body, picking up the eggs of one worm and the sperm of its mate. Circulatory System: 8 The pumping organs of the circulatory system are _____________________________. Circulatory fluids travel from the arches through the _______________________ to capillary beds in the body. The fluids then collect in the ____________________ and reenter the aortic arches. Earthworms have a closed circulatory system because the blood is always contained within either the heart or blood vessels. This allows blood to flow more rapidly than in an open circulatory system. Digestive System: The earthworm takes in a mixture of soil and organic matter through its ____________, which is the beginning of the digestive tract. The mixture enters the_____________, which is located in segments 1–6. The pharynx “sucks” food into the digestive tract. The ________________________, in segments 6–13, acts as a passageway between the pharynx and the crop. The ______________ stores food temporarily. The mixture that the earthworm ingests is ground up in the ______________________. In the _________________________, which extends over two-thirds of the body length, digestion and absorption take place. Soil particles and undigested organic matter pass out of the worm through the ____________________________________. Nervous System: The nervous system consists of the ____________________________, which travels the length of the worm on the ventral side, and a series of ______________, which are masses of tissue containing many nerve cells. The ___________________ surrounds the pharynx and consists of ganglia above and below the pharynx. Nervous impulses are responsible for _________________________________________. Each segment contains an enlargement, or __________________, along the ventral nerve cord. Label the earthworm. 9 There are 4,400 species of worms - 2,700 different kinds of earthworms to be exact. Worms are a varied lot. You may have heard of roundworms, flatworms, tapeworms, earthworms, and who knows what other kinds of worms. None of them conjures up a particularly warm or pleasant feeling in most people. Worms have low reputations in human circles, often associated with some not-so-pleasant circumstances. But this activity may turn all that around as you dig into the subject of earthworms. Earthworms are members of the phylum Annelida, or ringed animals. They are fairly simple life-forms, put together from a number of disk-like segments stuck together like a long flexible roll of coins. Earthworms have no internal skeleton like a fish, no hard protective exoskeleton like an insect, and no shell into which they can withdraw. Worms are flexible, elongated bundles of muscle, uniquely suited for life underground. The characteristic wriggling of earthworms is accomplished by the contraction of two kinds of muscles. When the short muscles that circle each segment (like lots of rings on a finger) contract, the worm gets thinner and longer. When the long muscles that connect all the segments contract, the head and tail are pulled toward each other, and the worm becomes short and fat. Depending on which end of the worm is anchored, the worm can move along the surface of the ground or through its burrow effectively in either direction, head first or tail first. Earthworm organs are quite different from ours, making it possible for them to live their very different lifestyle efficiently. Earthworms have five pairs of simple hearts that pump blood throughout the body. They have no lungs. Instead the blood flowing close to the worm's surface absorbs oxygen and releases carbon dioxide directly through the moist skin (called the cuticle). For this reason earthworms can live for some time in water if the oxygen supply is adequate. They don't drown per se, but they may suffocate if the oxygen content is low. This is why worms leave the soil and crawl out on the sidewalk during a heavy rain—they are seeking oxygen. Earthworms are not adapted to feed in water, however, so they would starve to death in due course. Instead of a nose, ears, and eyes, earthworms have a nervous system throughout their bodies that controls actions in response to environmental stimuli, such as vibrations, heat, cold, moisture, light, and the presence of other worms. They have no brain, however, so worms do not ponder their lowly lot in life, nor do they plan a strategy for obtaining their next meal or crossing the sidewalk safely. 10 Body Parts Pharynx: I push my pharynx or throat out of my mouth to grab leaves and to pull them back into my mouth. Then I get them nice and wet with my saliva. Esophagus: Once I have my food good and wet, I push it down my esophagus, then onto my crop. Crop: My crop is a storage compartment for my food and other things I swallow. From the crop, my lunch goes to my gizzard. Gizzard: My gizzard is where the work happens. I use any stones that I've swallowed and the strong muscles of my gizzard to grind up the leaves. These muscles work almost like teeth. Intestine: Once I have the leaves all ground up they move to my intestine where the digestive juices break them down even more. Anus: Whatever is leftover comes out my anus as castings or worm poop. Food. Earthworms feed on decomposing organic material, mostly vegetation, from the surface of the soil and within the soil itself. In the process of burrowing and feeding they process tons of soil in a typical pasture or garden, improving the quality of soil for plants and other animals. There are some 1800 species of earthworms worldwide. Some are tiny, no more than 2 cm (1”) at maturity. At the other end of the scale are the Australian giants that average about 3 m (10’) in length, and the record holder, a South African gargantuan measuring 7 m (22’) in length. Not to worry—the largest earthworms in North America are the common night crawlers, which can reach a length of little more than 30 cm (12”). Questions: 1. How do earthworms wiggle? _________________________________________________ 2. How many hearts does an earthworm have? _____________________________________ 3. How does an earthworm breathe? _____________________________________________ ___________________________________________________________________________ 4. Why do earthworms crawl out onto your driveway after a long rain? ___________________ ___________________________________________________________________________ 5. How does an earthworm know your coming if they don’t have eyes to see you?__________ ___________________________________________________________________________ 6. What is the pathway of the digestive system after food enters the mouth? ______________ ___________________________________________________________________________ 7. What is the job of the pharynx? _______________________________________________ 8. What is the job of the esophagus? _____________________________________________ 9. What is the job of the crop? __________________________________________________ 10. What is the job of the gizzard? _______________________________________________ 11. What is the job of the intestine? ______________________________________________ 12. What does an earthworm eat? _______________________________________________ 11 Earthworm Anatomy Lab External Anatomy 1. Examine your earthworm and determine the dorsal and ventral sides. Locate the two openings on the ventral surface of the earthworm The openings toward the anterior of the worm are the sperm ducts The openings near the clitellum are the genital setae. 2. Locate the dark line that runs down the dorsal side of the worm, this is the dorsal blood vessel. The ventral blood vessel can be seen on the underside of the worm, though it is usually not as dark. 3. Locate the worm's mouth and anus. Note the swelling of the earthworm near its anterior side - this is the clitellum. Internal Anatomy 1. Place the specimen in the dissecting pan DORSAL side up 2. Locate the clitellum and insert the tip of the scissors about 3 cm posterior. 3. Cut carefully all the way up to the head. Try to keep the scissors pointed up, and only cut through the skin. 4. Spread the skin of the worm out, use a teasing needle to gently tear the septa (little thread like structures that hold the skin to organs below it) 5. Place pins in the skin to hold it apart, Reproductive System The first structures you probably see are the seminal vesicles. They are cream colored and located toward the anterior of the worm. These are used for producing sperm. Use tweezers to remove these white structures from over the top of the digestive system that lies underneath it. 12 Circulatory system The dorsal blood vessel appears as a dark brownish-red vessel running along the intestine. The heart (or aortic arches) can be found over the esophagus (just posterior to the pharynx). Carefully tease away the tissues to expose the arches of the heart, the run across the worm. If you are careful enough, you can expose all 5 of them The ventral blood vessel is opposite the dorsal blood vessel, and cannot be seen at this time because the digestive system covers it 1. Label the diagram (use the bold words from above) 2. Does the earthworm have a closed or open circulatory system? ________________ Digestive System The digestive system starts at the mouth. You will trace the organs all the way to the anus and identify each on the worm. Find the mouth opening, the first part after the mouth is the pharynx, you will see stringy things attached to either side of the pharynx (pharyngeal muscles). The esophagus leads from the pharynx but you probably won’t be able to see it, since it lies underneath the heart. You will find a two structures close to the clitellum. First in the order is the crop, followed by the gizzard. The gizzard leads to the intestine which is as long as the worm and ends at the anus. Describe the functions of each of the organs and label them on the drawing. (The words are listed for you) Crop ______________________________________________________________________ Mouth ______________________________________________________________________ Pharynx ____________________________________________________________________ Intestine ___________________________________________________________________ Gizzard ____________________________________________________________________ 13 Anus ____________________ ________________________ ________________________ Esophagus ______________ ________________________ ________________________ Pharyngeal Muscles ________________________ ________________________ LABEL THE DIGESTIVE SYSTEM OF THE EARTHWORM PICTURED *Use your scissors to cut open the crop and the gizzard. In which organ would you expect the contents to be more ground up. Organ systems For the picture below, color code the organ systems for the earthworm using the following key: Circulatory System – Red Reproductive System – Blue Digestive System - Green Nervous System - Yellow 14 BRAINPOP ACTIVITY: CELL STRUCTURES 15 Animal and Plant Cells Eukaryotic cell: ____________________________________________________ Examples: _____________________________________________________ Prokaryotic cell: ___________________________________________________ Examples: _____________________________________________________ Organelle: _________________________________________________________ Semi-Permeable (Selectively Permeable) _________________________________ __________________________________________________________________ Cytoplasm: _______________________________________________ ________________________________________________________ ________________________________________________________ Cell Membrane: ___________________________________________ ________________________________________________________ ________________________________________________________ Cell Wall: _______________________________________________________ ________________________________________________________ ________________________________________________________ Cytoskeleton: ____________________________________________________ ________________________________________________________ ________________________________________________________ Nucleus: ________________________________________________________ ________________________________________________________ ________________________________________________________ Nuclear Membrane: ________________________________________________ ________________________________________________________ ________________________________________________________ Nucleolus: _________________________________________________ ________________________________________________________ Nuclear Membrane: _______________________________________________ 16 ________________________________________________________ ________________________________________________________ Ribosomes: _________________________________________________ ________________________________________________________ ________________________________________________________ Endoplasmic Reticulum (E.R.): _______________________________ ________________________________________________________ ________________________________________________________ Mitochondria: _______________________________________________ ________________________________________________________ ________________________________________________________ Chloroplast: ________________________________________________ ________________________________________________________ ________________________________________________________ Golgi Body: ________________________________________________ ________________________________________________________ ________________________________________________________ Vacuole: __________________________________________________ ________________________________________________________ ________________________________________________________ Lysosome: _________________________________________________ ________________________________________________________ Label the animal cell 1. 13. 12. 17 2 Part B. Beside each organelle describe it’s function: Cell membrane ____________________________________________________ Cytoplasm ____________________________________________________ Nucleus ____________________________________________________ Nuclear Membrane ____________________________________________________ Nucleolus ____________________________________________________ Lysosome ____________________________________________________ Mitochondria ____________________________________________________ ER ____________________________________________________ Golgi Body ____________________________________________________ Ribosomes ____________________________________________________ Vacuole ____________________________________________________ Cytoskeleton ____________________________________________________ Label the Plant cell 1. 15. 18 Part B. Beside each organelle create an anology: Cell membrane ____________________________________________________ Cytoplasm ____________________________________________________ Nucleus ____________________________________________________ Nuclear Membrane ____________________________________________________ Nucleolus ____________________________________________________ Lysosome ____________________________________________________ Mitochondria ____________________________________________________ ER ____________________________________________________ Golgi Body ____________________________________________________ Ribosomes ____________________________________________________ Vacuole ____________________________________________________ Cytoskeleton ____________________________________________________ Chloroplast ____________________________________________________ Cell Wall ____________________________________________________ CELL PROJECT: You may choose any one of the three projects below. 19 1. Write a cell song. There must be a verse for each of the 14 organelles you’ve learned about. Your song must be typed, and you must include a labeled drawing of a plant cell. The words can go to a song you know or you can create the song on garage band. For example: Bicher had a little cell, little cell, little cell Bicher had a little cell that was full of organelles. The nucleus is the brain, is the brain, is the brain the nucleus is the brain, it controls the cell. (To the tune of Mary had a little lamb) 2. Plant cell collage Draw, label, and color an animal cell. Then find pictures of vaious objects that perform the same type of job as the structure within the cell. All 14 organelles must be drawn and labeled. All 14 organelles must also have a picture and explanation comparing their job to the cells job. Please see the back wall for some examples. 3. Wanted posters For this option you will create various “wanted” posters. You need a “poster” for each organelle. For this option, you will use Power Point. Each slide will represent a poster for your organelle. Only choose this option if you feel comfortable using PowerPoint. RUBRIC: 10 pts All organelles are included with correct job descriptions Project is neatly done, and easy to read. Directions were followed Spelling and grammar is good, less than 2 mistakes 5 pts 0 pts Less than 4 organelles are missing or incorrect Project not done There are fewer than 3 scribbles. Project is a bit haphazard. Project not done Grammar and spelling has several errors. Students should have read first! Project not done BRAINPOP ACTIVITY: Active Transport 20 Cell Transport 21 Diffusion:___________________________________________________________ Semi-permeable membrane Which way will the small black molecules move? Which way will the large white molecules move? Osmosis: ___________________________________________________________ Equillibrium: ________________________________________________________ Hypertonic: ________________________________________________________ Salt Water solution 70% H20 30 % NaCl Red Blood Cell 95 % H2O 5% Nacl Hypotonic: __________________________________________________________ Is the red blood cell above hypertonic or hypotonic? Is the salt water solution above hypertonic or hypotonic? Cell Transport Use arrows to show which way the molecules are moving. 22 1. Hypertonic or hypotonic 25% H2O 50% NaCl 25% O2 2. Hypertonic or hypotonic 50% H2O 20% NaCl 30% O2 30% H2O 40% NaCl 20% O2 10% CO2 3. Hypertonic or hypotonic 15% H2O 50%NaCl 5% Fe 30% O2 50% H2O 10% NaCl 25% O2 15% CO2 4. Hypertonic or hypotonic 25%H2O 50% NaCl 15% Fe 10% O2 40%H2O 10% NaCl 5% Fe 25% O2 20%CO2 50% H2O 15% NaCl 5% Fe 15%O2 15% CO2 Fill in the missing percentages to make the following diagrams work. 5. Hypertonic or hypotonic 6. Hypertonic or hypotonic 10% H2O 50% NaCl 40% O2 20% H2O ____%NaCl 50% O2 25% H2O 25% NaCl 50% O2 7. Hypertonic or hypotonic ______% H2O ______%Fe ______% NaCl _____% O2 8. Hypertonic or hypotonic 25% H2O 25% Fe 25% NaCl 25% O2 ____%H2O ____% Fe ____%NaCl ____%O2 9. Hypertonic or hypotonic ____%H20 50% NaCl _____%Fe 20%H2O ____%NaCl 60% O2 20%H2O 20% Fe 20%NaCl 40% O2 10. Hypertonic or hypotonic ____%H2O 25% NaCl _____%Fe _____%H2O 25% NaCl _____% Fe ____%H2O 50%NaCl _____%Fe Follow the directions for each situation and set up the cell to make the movement work. 23 11. CO2 and O2 enter the cell NaCl and H2O leave the cell 12. O2, CO2, and H2O enter the cell NaCl leaves the cell 13. CO2 and O2 enter the cell NaCl is in equilibrium H2O leaves the cell 14. H2O leaves the cell Fe enters the cell and CO2 is in equilibrium 15. CO2 and H2O enter the cell NaCl and O2 leave the cell Fe is in equilibrium 16. NaCl and CO2 enter the cell O2 and H2O leave the cell Fe is in equilibrium Osmosis and Diffusion Lab Part A Diffusion 24 Purpose: In this lab, students can see firsthand the diffusion of a substance across a semipermeable membrane. Materials: • tincture of iodine • cornstarch/water solution • dialysis tubing • beakers Procedure: 1. Fill beaker about halfway and put about ten drops of iodine. 2. Put a teaspoon of starch and about 50 ml of water in dialysis bag and tie 3. Put the baggie in the beaker and wait. After 10 minutes observe the baggie and the beaker. Note any changes in the data table below. Data: HYPOTHESIS After 2 min. After 5 min. After 10 min. What changes do you think you will observe? Changes observed Discussion Questions: 1. The plastic bag is permeable to which substance? ___________________________ 2. Why did the iodine enter the bag? ______________________________________ __________________________________________________________________ 3. Why didn't the starch enter the beaker?_________________________________ __________________________________________________________________ 4. How is the plastic bag like the cell membrane? _____________________________ __________________________________________________________________ PART B: OSMOSIS In this investigation you will use a fresh egg to determine what happens in osmosis. You will be measuring the amount of water that passes through the membrane lining the shell of th3 egg. 25 MATERIALS: fresh egg in shell wax pencil, 200 mL graduated cylinder 3 jars with covers white vinegar, clear sugar syrup (Karo, for example), distilled or bottled water. DAY 1 PROCEDURE: 1. With the wax pencil, label the three jars: vinegar, syrup, water. Also put the number of your group on each jar. 2. Using the graduated cylinder, measure out 200 mL of vinegar. Put it in the jar labeled "vinegar". 3. Place the egg in the jar. The vinegar should cover the egg. Cover the jar with the lid but do not screw it on tightly. 4. Put the jar in the plastic tray and allow it to stand for 24 hours. DAY 2 PROCEDURE: 5. Observe what has happened to your egg. Record in Figure 1 of the data sheet. 6. Using the graduated cylinder, measure 200 mL of syrup and pour it into the correct jar. 7. Carefully remove the egg from the vinegar. It is quite fragile now as the shell is dissolved. Very gently rinse the egg in water and place it in the syrup jar. Put the cover on loosely. 8. Using the graduated cylinder, measure the amount of vinegar left in the vinegar jar. (If any vinegar has overflowed into the tray, include it in the measurement.) Record the volume of remaining vinegar in Figure 1 on the data sheet. DAY 3 PROCEDURE: 9. Measure 200 mL of water and add it to the water jar. 10. Carefully remove the egg from the syrup jar. Record your observations of the egg's appearance in Figure 1 on the data sheet. 11. Place the egg in the water jar. Cover loosely. Allow to stand for 24 hours. 12. Measure the volume of liquid that remains in the syrup jar. (If any has spilled out, include it in the measurement.) Day 4 PROCEDURE: 13. Remove the egg from the water and record your observations of its appearance. Discard the egg in the container provided. 14. Measure the amount of water that is left in the jar. Record in the data table. 15. Answer the questions on the data sheet. Data Table: 26 Volume before egg was added Volume after egg was added Observations of egg after 24 hours. Vinegar Syrup Water QUESTIONS: 1. When the egg was placed in the vinegar in which direction did the water molecules move? _______________________________________________________________________ _______________________________________________________________________ 2. On what evidence do you base this? _______________________________________________________________________ _______________________________________________________________________ 3. How do you explain the volume of liquid remaining when the egg was removed from the syrup? _______________________________________________________________________ _______________________________________________________________________ 4. When the egg was placed in the water after being removed from the syrup, in which direction did the water move? Explain your answer. _______________________________________________________________________ _______________________________________________________________________ PART C: PLASMOLYSIS The purpose of this activity is to investigate the effects of a hypertonic solution on the cells of the red onion. MATERIALS (per student): red onion epidermis, forceps, dropper, distilled water, 5% Sodium Chloride (table salt) solution, paper towels, microscope, PROCEDURE: 1. Make a wet mount of the red onion epidermis. 27 slide cover slip 2. Examine under 100X. When you have a clear view of several cells, switch to 430X. Make a colored drawing, properly labeled in the first circle on the data sheet (Fig. 2). 3. Begin to drop some of the salt solution under one side of your cover slip while placing a small piece of paper towel along the opposite edge of the cover slip. The paper should draw out the water and draw in the salt solution. 4. Observe the effects of the solution on the onion cells. Make a properly labeled, colored drawing of the cells' appearance in the second circle on the data sheet (fig. 3). 5. Replace the sodium chloride solution with distilled water in the same way that the salt solution was added. Make a properly labeled, colored drawing of the cells' appearance in the third circle on the data sheet (Fig. 4). 6. Answer the questions on the data sheet. Figure 2 Figure 3 Figure 4 PART C: PLASMOLYSIS QUESTIONS: 1. Which of the two liquids was hypotonic? _______________________________________________________________________ _____________________________________________________________ 2. On what evidence do you base this? _______________________________________________________________________ _____________________________________________________________ 3. Which of the two liquids was hypertonic? _______________________________________________________________________ _____________________________________________________________ 4. On what evidence do you base this? _______________________________________________________________________ _____________________________________________________________ APPLICATIONS: Using concepts developed from the tHREE experiments, Answer the questions 28 of application. 1. Why do grocery store owners spray fresh fruits and vegetables with water? _______________________________________________________________________ _____________________________________________________________ 2. Roads are sometimes salted to melt ice. What does this do to plants around the roadside and why? _______________________________________________________________________ _____________________________________________________________ 3. If a shipwrecked crew drinks sea water, they will probably die. Why? _______________________________________________________________________ _____________________________________________________________ 4. If a bowl of fresh strawberries is sprinkled with sugar, a few minutes later the berries will be covered with juice. Why? _______________________________________________________________________ _____________________________________________________________ Photosynthesis and Cellular Respiration 29 Photosynthesis: ______________________________________________________ __________________________________________________________________ _____________+___________________+____________________ _____________+___________ Cellular Respiration: ___________________________________________________ __________________________________________________________________ ___________________________+____________ ___________+_____________+______________ Fermentation: ______________________________________________________ __________________________________________________________________ What are the products of photosynthesis? __________________________________ What are the products of cellular respiration? _______________________________ What are the reactants of photosynthesis? __________________________________ What are the reactants of cellular respiration? _______________________________ Photosynthesis 30 Background Information: PHOTOSYNTHESIS is the process during which a plant's chlorophyll traps light energy and sugars are produced. In plants, photosynthesis occurs only in cells with chloroplasts. The chemical reaction for photosynthesis is: chlorophyll 6CO2+6H2O+light energy C6H12O6+6O2 Green plants use energy from light to combine carbon dioxide and water to make food. Light energy is converted to chemical energy and is stored in the food that is made by green plants. The light used in photosynthesis is absorbed by a green pigment called chlorophyll. Each food-making cell in a plant leaf contains chlorophyll in small cells called chloroplasts. In chloroplasts, light energy causes water drawn from the soil to split into molecules of hydrogen and oxygen. In a series of chemical reactions, the hydrogen combines with carbon dioxide from the air, forming a simple sugar. Oxygen from the water molecules is given off in the process. From sugar, along with nutrients from the soil, green plants can make starch, fat, protein, vitamins, and other complex compounds necessary for life. Photosynthesis supplies the chemical energy needed to produce these compounds. Problem: To observe evidence of photosynthesis in a water plant. Materials: 31 Part 1 Procedure: 1. Read the procedure carefully. What will you be observing? Make a chart in the data section to record this data. 2. Observe as a classmate blows through a straw into the flask of indicator solution. Record your observations. 3. Use the masking tape to label 3 test tubes with 1, 2, 3 and your group number. 4. Fill 3 test tubes ½ way with the indicator solution. 5. Put a sprig of Elodea in test tubes 1 and 2. Do put anything in test tube 3. 6. Stopper all three test tubes. 7. Place test tubes 1 and 3 in bright light. Place test tube 2 in the dark. 8. Leave the test tubes overnight; record your observations the following day. Data: Data Analysis: 1. Which test tube(s) showed a color change in this investigation? 32 2. What does a color change indicate in this investigation? Conclusion: 1. Write a short paragraph explaining the results of this investigation. Provide evidence from the investigation to support what you say. Questions: 1. What is an indicator? 2. Why did the indicator solution change colors? 3a. Independent variable ____________________________ 3b. Dependent variable ______________________________ Part 2: Rate of photosynthesis Procedure: 1. Obtain a sprig of Elodea. 2. Remove several leaves from around the end of the stem. Cut the stem at an angle. Lightly crush the end of the stem. 3. Fill a large test tube ¾ full of distilled water. Add a pinch of baking soda. 4. Put the Elodea in the test tube, stem side up. 5. Set the test tube in a test tube rack. 6. Place a 40 watt lamp 5 cm from the plant. 7. After 1 minute, count and record the number of bubbles coming from the crushed end of the Elodea stem. Count the bubbles produced each minute for 5 minutes. Record results. 8. Make qualitative observations during this investigation. Record these results. 9. Record the results for each group in the class. 10. Move the lamp so that it is 20 cm from the plant. After 1 minute, count and record bubbles produced each minute for 5 minutes. 11. Make qualitative observations during this investigation. Record these results. 12. Record your results. Record the results of your classmates. DATA: 33 Make a double line graph to show the averages data. REMEMBER TITLES & LABELS!! 34 Data Analysis: 1. How does the rate of photosynthesis change when the distance of the light source changes? 2. What does the graph tell us about the rate of photosynthesis at different distances from the light source? Conclusion: 35 1. Explain why the plant was producing bubbles when placed near the light source. 2. How does this investigation show that that plants give off oxygen during photosynthesis? Use evidence from the investigation to support your answer. Questions: 1. What is the independent variable in this investigation? 2. What is the dependent variable in this investigation? 3. What are some controlled variables in this investigation? 4. Why did we average all the trials in the class to analyze the data? 5. Describe the photosynthesis equation in words. Cell Reproduction 36 _________________________The heredity material that controls all cell activities, including making new cells. _____________________________________________Long strands of DNA Humans have ________________ chromosomes. Fruit flies have _______________chromosomes. Potatoes have ________________ chromosomes. ___________________________________________Asexual reproduction ___________________________________________ means “splitting into two parts” _________________________ have a single circular DNA molecule. Binary fission results in two cells that each contain _____________ copy of the circle of DNA. _____________________________________A type of asexual reproduction Mitosis is a type of cell division where ______________________________________ are formed ____________________________________________. There are 6 stages or phases to mitosis: 1st: ___________________ Chromosomes are _________________ (# doubles) Chromosomes appear as threadlike coils (__________________) at the start, but each chromosome and its copy (__________________ chromosome) change to sister chromatids at the end of this phase. __________________ 2nd: ___________________ _________________ begins (cell begins to divide) _________________ (or poles) appear and begin to move to opposite ends of cell. _____________ _____________ form between the poles. 3rd: ___________________ 37 __________________ (or pairs of chromosomes) attach to the spindle fibers. The _____________________ line up in the _________________ of the cell. 4th: ___________________ Chromatids (or pairs of chromosomes) ________________ and begin to move to _____________________ ends of the cell. 5th: ___________________ Two new ____________________________ form. Chromosomes appear as chromatin (__________________ rather than __________________) ______________________ ends. Cell membrane moves inward to create two _____________ 6th: ___________________ cells – each with its own _____________________ with identical ___________________________. Cell Growth and Division 38 Write the name of the phase of the cell cycle next to each event described below. __________________________1. Centromeres divide __________________________2. Centrioles move to opposite ends of the cell. __________________________3. Nuclear membrane forms around each mass of chromosomes. __________________________4. Chromosome strands separate toward opposite ends of the cell. __________________________5. A copy of each chromosome is made. __________________________6. Centromeres attach to the spindle fibers. __________________________7. The nuclear membrane disappears. __________________________8. The material in the nucleus that appears grainy condenses to become visible as chromosomes. __________________________9. Double stranded chromosomes line up in the center of the cell. __________________________10. Chromatin condense and become visible. Injured and old, worn-out cells in your body are constantly being replaced by new cells. New cells are produced by the processes of mitosis and cell division. Study the four stages of mitosis shown in the pictures. Number the stages in the order in which they occur. QuickTime™ and a TIFF (Uncompressed) decompressor are needed to see this picture. QuickTime™ and a TIFF (Uncompressed) decompressor are needed to see this picture. QuickTime™ and a TIFF (Uncompressed) decompressor are needed to see this picture. QuickTime™ and a TIFF (Uncompressed) decompressor are needed to see this picture. 11. How do mitosis and cell division differ in animal cells and plant cells? ____________ __________________________________________________________________ Mitosis Lab Activity 39 http://bio.rutgers.edu/~gb101/lab2_mitosis/section2_frames.html Please go to the URL listed above. Follow the directions on the web page and go through the lab. In the spaces below draw each stage of mitosis, Onion root tip: Interphase Prophase Metaphase Anaphase Telophase Whitefish blastulae 40 Prophase Metaphase Anaphase Telophase Interphase What is Meiosis? 41 __________________________________________________________________ __________________________________________________________ Meiosis involves a _______________________________________________ Meiosis consists of _______________________________________________ but only copies the ________________________________. Meiosis produces ________________________________________________ What is a haploid daughter cell? A haploid daughter cell contains ___________________________________. Therefore each ___________________________________ cell for a human would have only _________________________________________. __________________________________________________________________ __________________________________________________________ What is a diploid daughter cell? A diploid daughter cell contains _____________________________________. Therefore each __________________________________________________ Diploid daughter cells are the result of ________________________________ Haploid daughter cells are the result of ________________________________ Stages of Meiosis 1st: ___________________ Chromosomes are _________________ (# doubles) Chromosomes appear as threadlike coils (__________________) at the start, but each chromosome and its copy (__________________ chromosome) change to sister chromatids at the end of this phase. 2nd: ___________________ _________________ begins (cell begins to divide) _________________ (or poles) appear and begin to move to opposite ends of cell. _____________ _____________ form between the poles. 42 _____________ __________ occurs during prophase. This is when a pair of homologous chromosomes “cross over” . ______________ _____________ is the change of genetic material (________) between these two homologous chromosomes. This process contributes to ______________ ______________________ 3rd: ___________________ __________________ (or pairs of chromosomes) attach to the spindle fibers. The _____________________ line up in the _________________ of the cell. 4th: ___________________ Chromatids (or pairs of chromosomes) ________________ and begin to move to _____________________ ends of the cell. 5th: ___________________ Two new ____________________________ form. Chromosomes appear as chromatin (__________________ rather than __________________) This is the end of the first nuclear division. Cell membrane moves inward to create two _____________ 6th: ___________________ cells – each with its own _____________________ with identical ___________________________. 7th: ___________________ Chromosomes _________________________________ 43 8th: ___________________ ____________________________________ disappears In animal cells the centrioles move to opposite sides of the cell. 9th: ___________________ Chromosomes _________________________________ The sister chromatids _________________________________ 10th: ___________________ 11th: ___________________ 44 cell membrane pinches, division is complete 4 ___________________________________________ Haploid cells ___________________________________ _____________________________________________ NOVA 45 http://www.pbs.org/wgbh/nova/miracle/divide.html Go to the above website and read the tutorial on mitosis and meiosis. Use the tutorial to help you determine the similarities and differences between mitosis and meiosis, and fill in the chart below. Mitosis What type of cells use this type of cell division? Are the daughter cells genetically identical to the parent cell? Are the daughter cells haploid or diploid? How many chromosomes does each daughter cell have? How many daughter cells are produced? 46 Meiosis To understand how an extra copy of one chromosome could result in abnormalities, remember that each chromosome has genes with the instructions to make specific types of proteins, so the extra chromosome could result in too many copies of these particular proteins. Think about what might happen if you added too much milk to a box of macaroni and cheese. The macaroni and cheese would have too much liquid and be runny instead of creamy. Cells are much more complicated than mac and cheese, and a cell cannot function properly when there are too many copies of some types of proteins due to an extra copy of one of the chromosomes. When the cells in an embryo do not function properly, the embryo may develop abnormalities and often dies. 47 48 49 50 Generations of Traits - Instructions In this activity you will track different traits (represented by colored pom-poms) through three generations of “Ginger People”. You will need the Generations of Traits Worksheet to follow along. 1. With a partner, label six cups as shown: 2. Arrange the cups as shown above and place six beans in the cups, following the directions below: Grandfather A – red kidney bean Grandfather B – white bean Grandmother A – brown bean Grandmother B – green pea The colored beans are the traits that each of the grandparents have. Color the circles on the Generations of Traits Worksheet to show the traits for each grandparent. 3. Close your eyes and pick three traits from Grandfather A and three traits from Grandmother A and place them in the cup labeled Mother. These are the traits that mother inherited from her parents. Color the circles on the worksheet to show the traits mother has. 4. Close your eyes again and pick three traits from Grandfather B and three traits from Grandmother B, and place them in the cup labeled father. These are the traits that father inherited from his parents. Color the circles on the worksheet to show the traits father has. 5. Mother and Father have four children: Mary, George, Elizabeth and Carl. To determine the traits that Mary will inherit from mother and father, close your eyes and take three beans from mother and three beans from father. Color the diagram to show the traits Mary inherited. 6. Next, return the traits that you took from mother and father. (Look at your diagram if you forget where each trait came from.) Now, close your eyes again and choose the traits that George will inherite (3 from mother, 3 from father). Color the diagram to show George’s traits. 7. Return the traits you took from mother and father and repeat the process to find the traits for Elizabeth and then Carl. 8. Answer the questions on the Generations of Traits Question shet. 51 Generations of Traits – Worksheet 52 Generations of Traits – Questions 1. Would Mary, George, Elizabeth and Carl look identical to their parents? In other words would they have the same traits as their parents? 2. Did all four children inherit exactly the same traits or is there some variation? 3. How many of the four children inherited a trait from each one of the grandparents? 4. Is there a child that didn’t inherit a particular trait? If so, which trait (color) was it? 53 GENETICS Gregor Mendel - ___________________________________________________________ _______________________________________________________________________ Trait - __________________________________________________________________ _______________________________________________________________________ Inherited trait - __________________________________________________________ _______________________________________________________________________ Acquired trait - ___________________________________________________________ _______________________________________________________________________ Dominant trait - ___________________________________________________________ _______________________________________________________________________ Recessive trait - __________________________________________________________ _______________________________________________________________________ Homozygous trait - _________________________________________________________ _______________________________________________________________________ Heterozygous trait - _______________________________________________________ _______________________________________________________________________ Genotype - ______________________________________________________________ _______________________________________________________________________ Phenotype - ______________________________________________________________ _______________________________________________________________________ 54 Part A: Smiley Face Traits (1) Obtain two coins from your teacher. Mark one coin with a “F” and the other with a “M” to represent each of the parents. The parents are heterozygous for all the Smiley Face traits. (2) Flip the coins for parent for each trait. If the coin lands with heads up, it represents a dominant allele. A coin that lands tails up indicates a recessive allele. Record the result for each person by circling the correct letter. Use the results and the Smiley Face Traits page to determine the genotype and phenotype for each trait. Part B: Is it a boy or girl? To determine the sex of your smiley face, flip the coin for the male parent. Heads would represent X, while tails would be Y. Part C: Create Your Smiley Face! Use the Smiley Face Traits chart and your results from Part A to create a sketch of your smiley face in the box. Once you have completed the sketch, use the drawing tools in Microsoft Word to create your smiley face! Two things to remember ... 1. Do not add color on the computer! Print a black and white copy and then use crayons or colored pencils to finish it. 2. Don’t forget to give your smiley face a name! You will also need to include your name as parent and your class hour. 55 Smiley Face Traits T. Trimpe 2003 http://sciencespot.net/ Face Shape Circle (C) Oval (c) Eye Shape Star (E) Blast (e) Hair Style Straight (S) Curly (s) Smile Thick (T) Thin (t) Ear Style Curved (V) Pointed (v) Nose Style Down (D) Up (d) Face Color Yellow (Y) Green (y) Eye Color Blue (B) Red (b) Hair Length Long (L) Short (l) Nose Color Red (RR) Orange (RY) Yellow (YY) Ear Color Hot Pink (PP) Purple (PT) Teal (TT) Sex 56 Freckles Present (F) Absent (f) To determine the sex, the flip the coin for the male parent. Heads equals X and tails equals Y. XX - Female - Add pink bow in hair XY - Male - Add blue bow in hair Genetics with a Smile Wrapping It Up! (1) How does your smiley face compare to the ones created by your classmates? (2) Which smiley face has the most dominant traits? _____________________ How many? ______ traits (3) Which smiley face has the most recessive traits? _____________________ How many? ______ traits (4) Which traits were a result of incomplete dominance? (5) What is the probability that a smiley face will have a green face? _____ out of _____ or ____ % (6) How many smiley faces have a green face, which is a recessive trait? _____ out of _____ or ____ % (7) How does your predicted probability for a green face (#5) compare to the actual results (#6)? Explain. (8) What is the probability that a smiley face will have an orange nose? _____ out of _____ or ____ % (9) How many smiley faces have an orange nose? _____ out of _____ or ____ % (10) How does your predicted probability for an orange nose (#8) compare to the actual results (#9)? Explain. 57