Lisa L. Drake 1200 S. Horseshoe Dr. Foster Hall rm. 220 Las Cruces, NM 88003 Phone: (505) 690-9840 Email: drlisa@nmsu.edu EDUCATION: Present Ph.D. Candidate in Biology (subfield: Cell and organismal biology) New Mexico State University (NMSU), expected diploma spring 2015 2011 Master of Science in Biology (subfield: Cell and organismal biology) New Mexico State University (NMSU) 2008 Bachelor of Science in Biochemistry, Minor in Forensic Science. New Mexico State University (NMSU) 2004 High School Diploma Capital High School Santa Fe, NM, Valedictorian RECENT HONORS AND AWARDS: 2012 Outstanding Graduate Student Award, NMSU 2011 Biology Department Teaching Award, NMSU 2009-present Biology Department Teaching Assistantship, NMSU 2004-2009 New Mexico Scholars Scholarship, NMSU 2004 Los Alamos National Laboratory Scholarship, Bronze recipient RESEARCH EXPERIENCE: 2009-present GRADUATE STUDENT, New Mexico State University. Advisor: Immo A. Hansen (Biology) Project: Preformed molecular biology lab techniques to study and characterize membrane transporters in Aedes aegypti mosquitoes. -Mosquito dissection, RNA isolation, RT-PCR, qPCR, SDS-PAGE, Western blot, gel extraction, mosquito microinjection with dsRNA, gene cloning into expression vectors, Xenopus laevis oocyte microinjection and protein expression, computer skills including Graphpad Prism, experience working with avian malaria, Plasmodium gallinacium and animal models (chickens) in a BSL-1 laboratory, Environmental Health and Safety (EHS) trained as well as radiation safety trained. - Laboratory performance included maintenance and upkeep of Aedes aegypti, Aedes albopictus, Culex quinquefasciatus, and Anopheles quadrimaculatus mosquito culture, experience working with the bed bug, Cimex lectularius. 2011 SAPPHIRE ENERGY SUMMER INTERN, Las Cruces, NM Supervisor: Robert McBride, Ph.D. Lisa L. Drake 4 Project: Crop protection and laboratory research assistant on the Research and Development team for algae-based green crude oil production. - Assisted in algae mini-pond experimental data collection. - Tasks included collection of pH, temperature, depth, optical density, total organic carbon and Photosynthetic Activity Monitor (PAM) readings, qPCR data, and microscopic observations for each algae pond. PUBLICATIONS: 2014 Benoit JB, Hansen IA, Szuter EM, Drake LL, Burnett DL, Attardo GM.. (2014). Emerging roles of aquaporins in relation to the physiology of blood-feeding arthropods. J Comp Physiol B. Jun 19. doi: 10.1007/s00360-014-0836. 2014 Benoit JB, Hansen IA, Attardo GM, Michalková V, Mireji PO, Bargul JL, Drake LL, Masiga DK, Aksoy S. (2014). Aquaporins are critical for provision of water during lactation and intrauterine progeny hydration to maintain tsetse fly reproductive success. PLoS Negl Trop Dis. Apr 24. doi: 10.1371/journal.pntd.0002517. 2014 Hansen IA., Attardo GM, Rodriguez SD, Drake LL. (2014). Four-way regulation of mosquito yolk protein precursor genes by juvenile hormone-, ecdysone-, nutrient-, and insulin-like peptide signaling pathways. Front Physio. Mar 20;5:103. 2013 Rodriguez SD, Brar RK, Drake LL, Drumm HE, Price DP, Hammond JI, Urquidi J, Hansen IA. (2013). The effect of the radio-protective agents ethanol, trimethylglycine, and beer on survival of X-ray-sterilized male Aedes aegypti. Parasites and Vectors. doi:10.1186/1756-3305-6-211. 2012 Drake LL, Price DP, Aguirre SE, Hansen IA. (2012). RNAi-mediated gene knockdown and in vivo diuresis assay in adult female Aedes aegypti mosquitoes. JoVE. Jul 14;(65):e3479) 2012 Carpenter VK, Drake LL, Aguirre SE, Price DP, Rodriguez SD, Hansen IA. (2012). SLC7 amino acid transporters of the yellow fever mosquito, Aedes aegypti and their role in fat body TOR signaling and reproduction. J Insect Physiol. Apr;58(4):513-22. 2011 Price DP, Nagarajan V, Churbanov A, Houde P, Milligan B, Drake LL, Gustafson JE, Hansen IA. (2011). The fat body transcriptomes of the yellow fever mosquito, Aedes aegypti, pre- and post- blood meal. PLoS One. 6(7):e22573. 2011 Hansen IA, Boudko DY, Shiao SH, Voronov DA, Meleshkevitch EA, Drake LL, Aguirre SE, Fox J, Attardo GM, Raikhel AS. (2011). AaCAT1 of the yellow fever mosquito, Aedes aegypti: A novel histidine-specific amino acid transporter from the SLC7 family. J Biol Chem. 286, 10803-13. 2010 Drake LL, Boudko DY, Marinotti O, Carpenter VK, Dawe AL, Hansen IA. (2010). The aquaporin gene family of the yellow fever mosquito, Aedes aegypti. PLoS One. 5(12):e15578. PRESENTATIONS AND POSTERS: 2014 “Expression and functional characterization of aquaporins in the yellow fever mosquito, Aedes aegypti”. Three Minute Thesis Competition, Las Cruces, NM, March 2014. (Lisa L. Drake, Stacy D. Rodriguez, Immo A. Hansen) Lisa L. Drake 3 2013 “The functional characterization of aquaporins 1, 4, and 5 of the yellow fever mosquito, Aedes aegypti”. NMSU Biosymposium, Las Cruces, NM, March 2010-2014. (Lisa L. Drake, Stacy D. Rodriguez, Immo A. Hansen) 2012 “Functional Characterization of Aquaporins in the Disease Vectors Aedes aegypti and Culex quinquefasciatus”. Entomological Society of America Annual Meeting, Knoxville, TN, November 2012; Reno, NV, November 2011; Entomological Society of America Southwest Branch Annual Meeting, Las Cruces, NM, February 2013. (Lisa L. Drake, Sarah E. Aguirre, Immo A. Hansen) 2008 “Aquaporins in the Malpighian tubules are essential for water transport in the yellow fever mosquito, Aedes aegypti”. American Society of Tropical Medicine and Hygiene Annual Meeting, Philadelphia, PA, December 2011; Atlanta, GA, November 2010; Washington D.C., November 2009. ((Lisa L. Drake, Stacy D. Rodriguez, Immo A. Hansen) TEACHING EXPERIENCE: 2009-present TEACHING ASSISTANT, Biology Department, NMSU. Courses: Cell and Organismal Biology Laboratory (BIOL 211GL), Molecular Biology Techniques Laboratory (BIOL 302) PROFESSIONAL SOCIETIES: 2011-present Entomological Society of America member REFERENCES: Dr. Immo A. Hansen Department of Biology New Mexico State University Las Cruces, NM 88003 Telephone: (575) -646-7719 E-mail: immoh@nmsu.edu Dr. Kathryn Hanley Department of Biology New Mexico State University Las Cruces, NM 88003 Telephone: (575) -646-4583 E-mail: khanley@nmsu.edu Dr. Dimitri Boudko Department of Physiology & Biophysics Rosalind Franklin University Chicago, IL 60064 Telephone: (847) 578-8359 Email: dmitri.boudko@rosalindfranklin.edu Dr. Robert McBride Sapphire Energy Lisa L. Drake Las Cruces, NM 88007 Telephone: (858) 366-2331 Email: Robert.mcbride@sapphireenergy.com 4