David & Matan The Nitrogen Cycle The nitrogen cycle is the global circulation of nitrogen from the environment to living organisms and back to the environment. It is the process by which nitrogen is converted between its chemical forms. There are five steps in the nitrogen cycle, in which nitrogen cycles between the abiotic environment and organisms: nitrogen fixation, nitrification, assimilation, ammonification, and denitrification. Bacteria are involved in all of these steps except for assimilation. Nitrogen fixation – conversion of gaseous nitrogen to ammonia Nitrification – soil bacteria perform this in two steps: 1. Soil bacteria convert ammonium to nitrate 2. Soil bacteria oxidize nitrite to nitrate. Assimilation – Plant roots absorb nitrate ammonia, or ammonium and assimilate the nitrogen of these molecules into plant proteins and nucleic acids. Ammonification – conversion of biological nitrogen compounds into ammonia and ammonium ions Denitrification – reduction of nitrate to gaseous nitrogen. Denitrifying bacteria reverse the action of nitrogen-fixing and nitrifying bacteria by returning nitrogen to the atmosphere.