National Centre for Marine Conservation and Resource Sustainability KQA207 General Microbiology Semester 1 / 2008 Unit Outline Dr Chris Burke © The University of Tasmania 2004 CRICOS Provider Code: 00586B Contact details Unit coordinator/lecturer Unit coordinator/lecturer: Chris Burke Campus: Launceston e-mail: C.Burke@utas.edu.au Website: MyLO Phone: 63243806 Fax: 63243804 Room number NH.S255 Consultation hours: Business hours unless otherwise noted Contents Contact details ................................................................................................................ 2 Unit description..................................................................................................... 4 Learning outcomes .............................................................................................. 4 Generic graduate attributes .......................................................................... 4 Prior knowledge &/or skills ............................................................................ 5 Learning resources required ......................................................................... 5 Occupational health and safety (OH&S) .............................................. 5 Further information and assistance ........................................................ 6 Details of teaching arrangements............................................................. 6 Learning expectations and strategies ..................................................... 6 Unit schedule .......................................................................................................... 8 Specific attendance/performance requirements................................ 9 Assessment schedule ............................................................................................. 9 Assessment details ................................................................................................. 9 How your final result is determined ....................................................... 11 Submission of assignments............................................................................ 11 Requests for extensions .................................................................................... 12 Penalties ................................................................................................................... 12 Review of results and appeals ...................................................................... 12 Academic referencing...................................................................................... 12 Plagiarism .............................................................................................................. 13 2 Withdrawing from a unit ................................................................................. 13 3 Unit description KQA207 General Microbiology Offered: Launceston semester 1 General Microbiology is an introductory unit that gives students an overview of microbes, in particular bacteria, as well as specific skills in handling and using microbial cultures. The philosophy of learning in this unit is one of directed student learning, so that a good understanding is obtained of the concepts presented. Students are expected to be actively involved in their learning, which will enhance not only their knowledge and understanding of microbiology, but also impart learning skills useful for future study. Practical classes take the form of a directed research project closely integrated with the theory course. In it students will practise the scientific method of: aim or hypothesis setting followed by experimental testing and evaluation. Students are encouraged to ask questions in lectures and tutorials and to suggest alternative topics for discussion in tutorials. Learning outcomes On successful completion of this unit, students should be capable of: 1) designing experiments to carry out and evaluate basic bacteriological techniques to identify and study the characteristics of an unknown culture. 2) demonstrating responsible and careful techniques in the handling of microorganisms; 3) accurately and precisely observing, recording and interpreting bacterial growth; 4) researching microbiological resources to: a) describe a broad overview of bacterial systematics; b) demonstrate understanding of the basic forms of catabolism performed by bacteria; c) demonstrate understanding of selected examples of microbial and viral pathogenicity in fish; d) discuss the importance of topical microbiological issues. Generic graduate attributes The University has defined a set of generic graduate attributes (GGAs) that can be expected of all graduates (see http://www.utas.edu.au/tl/policies/index.htm). Whilst undertaking this unit, students will progress in their development of the following attributes: 4 Knowledge: through developing a basic understanding of microbiology by collating and gathering relevant information from a variety of sources. This will be assessed by 3 project scientific reports and by students’ capacities to integrate and evaluate microbiological information from the scientific literature and from their own studies in a taxonomic description and in the final exam. Communication skills: by beginning to appreciate how to work successfully in a small group to achieve a common aim. This will be assessed by the results of individual project reports and the content of the group laboratory diary. Written communication will be further assessed by essays in the final exam. Problem-solving skills: by developing understanding of how to carry out scientific research (planning, execution and evaluation) and by learning basic practical techniques so that bacterial cultures can be studied. Also, through application of theoretical knowledge to interpretation of common issues affecting daily human life. This will be assessed through the project reports, the midsemester tests and in the final exam. Global perspective: by appreciating that microbes affect all aspects of life on Earth and starting to develop an understanding of how they do so. Social responsibility: through developing an appreciation of ethical work and research methods and applying these within the group project. By following, in all aspects of work, the university’s code of practice in relation to plagiarism. Prior knowledge &/or skills There are no set pre-requisites for this unit. Students are expected to be committed to actively involving themselves in learning the unit subject matter: both in terms of the scientific method and in the specialist knowledge of microbiology. Learning resources required Requisite texts Madigan, M.T. and Martinko, J.M., 2006. Brock: Biology of Microorganisms, 11th edition, Prentice-Hall, New Jersey. Microbiology Methods Manual, 2008. Study notes for General Microbiology 2008. Recommended reading In the laboratory or library: Bergey’s Manual of Systematic Bacteriology, 2nd edition, vol. 1, 2. Springer. LtnRes 579.3012 B496 2001 Bergey’s Manual of Systematic Bacteriology, 1st edition, vols 1 – 4. Williams and Wilkins. Bergey’s Manual of Determinative Bacteriology, 1st edition. Williams and Wilkins. Buller, N.B., 2004. Bacteria from fish and other aquatic animals: a practical identification manual. CABI publishers, 341 pp. 579.3176 B936b 2004 Cowan and Steel’s Manual for the identification of medical bacteria, 2nd ed. Cambridge University Press. Encyclopedia of Microbiology vols 1 - 4. J. Lederberg (ed.) Academic Press. Ref.579.03 E56 2000. Fin Fish Diseases. Jan. 1990 Proceedings vol. 128, Postgraduate Committee in Veterinary Science, Sydney University 1990. Fin Fish Workshop Jan. 1992 Proceedings vol. 182, Postgraduate Committee in Veterinary Science, Sydney University 1992. These volumes are also available in the library. Electronic resources There is a website for this unit via MyLO. Occupational health and safety (OH&S) The University is committed to providing a safe and secure teaching and learning environment. All students are provided with a School of Aquaculture Handbook in which 5 laboratory rules and safe operating procedures are described. Additionally, there are specific requirements of this unit and these will be described in the first practical period. For a general overview you should refer to the University’s policy at: http://www.admin.utas.edu.au/hr/ohs/pol_proc/ohs.pdf Further information and assistance If you are experiencing difficulties with your studies or assignments, have personal or life planning issues, disability or illness which may affect your course of study, you are advised to raise these with your lecturer in the first instance. There is a range of University-wide support services available to you including Teaching & Learning, Student Services, International Services. Please refer to the Current Students homepage at: http://www.utas.edu.au/students/ Should you require assistance in accessing the Library visit their website for more information at http://www.utas.edu.au/library/ Details of teaching arrangements Lecture Tutorials Practicals Monday 11 – 12 & 3 - 4 NH.S141.LT 7 Wednesday 9 – 11, NH.S150 {not every week, see schedule} Friday 10 – 1, M102 {not every week, see schedule} Learning expectations and strategies Learning Expectations The University is committed to high standards of professional conduct in all activities, and holds its commitment and responsibilities to its students as being of paramount importance. Likewise, it holds expectations about the responsibilities students have as they pursue their studies within the special environment the University offers. The University’s Code of Conduct for Teaching and Learning states: Students are expected to participate actively and positively in the teaching/learning environment. They must attend classes when and as required, strive to maintain steady progress within the subject or unit framework, comply with workload expectations, and submit required work on time. Learning strategies In this unit a significant proportion of learning will be achieved via collaboration of students working together in small groups in the laboratory project and in tutorials. Students will be taught how to work in groups in order to practise the scientific method by designing and carrying out a research project that is closely linked to the theoretical component of the unit. Students will work collaboratively to develop their theoretical knowledge of bacteria Information for Accessing MyLO Go to the UTas website and look at: www.utas.edu.au/coursesonline/ 6 This site is the general help and advice area to guide you in the use of MyLO, which is used to support many units taught at UTas. If you use a modem at home to connect to the internet or do not have an internet connection at home, then I suggest that you buy a memory stick and download files from library computers or from computers in the AMC general computing laboratories. Lectures in LT7 will be recorded and made available in Lectopia in MyLO. See the web address below for general information about Lectopia. http://www.utas.edu.au/itr/lectopia/documents/StudentLectopiaSupport-v1.0.pdf 7 Unit schedule Week starting Lecture Topic / activity Number 25/02 Lectures 3/03 Tutorial Lectures 10/03 Tutorial Lectures Introduction to Course Introduction to microbiology Introduction to project E. coli and S. aureus – the lab rats. Researching bacterial cell structure Project planning, essay planning Public holiday Tutorial Researching bacterial cell structure Lectures Researching bacterial cell structure Bacterial growth & death Project planning: sub-project 2 Researching control of microbial growth 17/03 Tutorial Laboratory 24/03 No classes from 20/03/08 to 26/03/08 inclusive. 31/03 Lectures Tutorial 7/04 Lectures Tutorial 14/04 Lectures 21/04 Tutorial Lectures 28/04 Tutorial Lectures 5/05 Tutorial Lectures 12/05 Tutorial Lectures 19/05 Tutorial Lectures 26/05 Tutorial Lectures Tutorial Researching control of microbial growth An overview of microbial metabolism Project planning. Researching microbial metabolism. Bacterial taxonomy Researching bacterial diversity Researching bacterial diversity. Sub-project 2. Researching bacterial diversity Project planning & evaluation Midsemester test Researching bacterial diversity Researching bacterial diversity Researching bacterial diversity Project evaluation. Review of test Viruses Microbial pathogenesis None Overview of microbial diseases of fish Viral and other fish diseases None Bacterial diseases of fish Bacterial genetics – how bacteria change. None Researching genetic recombination Tying it all together Revision 1. No laboratory class 2. Laboratory procedures. Subproject 1: Survey of microbes. 3. Sub-project 1: Survey of microbes. Midsemester break over the Easter weekend. 4. Sub-project 1: Survey of microbes. 5. Sub-project 2: Bacterial taxonomy of purified cultures from sub-project 1. 6. Sub-project 2: Bacterial taxonomy of purified cultures from sub-project 1 7. Sub-project 2: Bacterial taxonomy of purified cultures from sub-project 1. 8. Sub-project 2: Bacterial taxonomy of purified cultures from sub-project 1. 9. Sub-project 2: Taxonomy. Sub-project 3: Environmental effects on microbial survival. 10. Sub-project 3: Environmental effects on microbial survival. 11. Sub-project 3: Environmental effects on microbial survival. 12. No laboratory class. 13. No laboratory class. NB: Detailed references for preparatory reading are given in the study guide. 8 Specific attendance/performance requirements Attendance at practical sessions is compulsory and absences must be explained to the lecturer, for example by providing a medical certificate if ill. Absence for more than 2 weeks may render a student ineligible to sit the final exam, resulting in a fail. Late assignments will incur a penalty of 10% of the total possible mark per day late. Assessment schedule Assessment task Date due Assignment 1: Sub-project 1 report Assignment 2: Taxonomic description Assignment 3: Sub-project 2 report Assignment 4: Mid-semester test Assignment 5: Completed practical schedule Assignment 6: Group laboratory diary Assignment 7: Sub-project 3 report Assignment 8: Online revision test End of semester exam 4/04/08 prac class 9/04/08 12 noon 2/05/08 prac class 23/04/08 tutorial 16/05/08 prac class 16/05/08 prac class 21/05/08 tutorial 21/06/08 12 am Percent weighting 4 10 8 10 5 5 8 5 45 Assessment details Assessment task 1: Sub-project 1: survey of microbes in the environment Task description Task length Links to unit’s learning outcomes Assessment criteria / guidelines Date due Scientific report evaluating methods and findings of sub-project 1. Report introduction, hypothesis, methods used, data obtained and a discussion addressing questions raised in project description (see Microbiology Laboratory Guide). Report should be typed. No more than 1000 words in total. Aim to be concise. Learning outcomes 1, 2 and 3 Correct format of scientific report (see Student handbook). Experiments address hypothesis and discussion evaluates data appropriately. Consideration given to questions raised in Lab. Guide. 4/04/08 during practical class Assessment task 2: Taxonomic description Task description Task length Links to unit’s learning outcomes Assessment criteria / guidelines Date due Research information about a species of microbe (see KQA207 lecture notes for a list of possible taxa) and write an essay describing the organism: its cellular and metabolic features, distinguishing characteristics and anything that you consider to be of interest about it. Use the textbook and other library sources as required. I have additional papers available. 1500 words Learning outcome 3 and 4a, b, c. The issues listed in the Lecture notes booklet under Taxonomic description are addressed. Correct formatting for an essay and correct attribution of published work. 9/04/08 12 noon. 9 Assessment task 3: Scientific report on sub-project 2 Task description Task length Links to unit’s learning outcomes Assessment criteria / guidelines Date due Scientific report evaluating methods and findings of sub-project 2. Report introduction, hypothesis, methods used, data obtained and a discussion addressing questions raised in project description (see Microbiology Laboratory Guide). Report should be typed. No more than 1000 words in total. Aim to be concise. Learning outcomes 1, 2, 3 and 4a. Correct format of scientific report (see Student handbook). Experiments address hypothesis and discussion evaluates data appropriately. Consideration given to questions raised in Lab. Guide. 2/05/08 during practical class Assessment tasks 4: Midsemester test Task description Task length Links to unit’s learning outcomes Assessment criteria / guidelines Date due Answer questions on the theoretical and practical concepts presented in the course. This test is to inform students of their understanding of the subject and to identify misconceptions so that these may be corrected. 1 hour Learning outcomes 4a, b and c Correct answers to multiple choice questions 23/04/08 during tutorial Assessment task 5: Completed practical schedule Task description Task length Links to unit’s learning outcomes Assessment criteria / guidelines Date due Throughout the semester students are to have their practical work checked by the demonstrator, who will sign off and date the work once the student is competent at that technique. A list of techniques to be learnt is given in the Microbiology Laboratory Guide During laboratory classes Learning outcome 1, 2 and 3. Refer to practical schedule in Microbiology Laboratory Guide and to the Microbiology Methods Manual 16/05/08 during the practical class Assessment task 6: Group project work diary Task description Task length Links to learning outcomes Assessment criteria / guidelines Date due Through out the semester, work collaboratively with 2 other students to plan, execute and evaluate a project to isolate a particular organism from an environmental source and to characterise some properties of your organism. Use a laboratory diary to organise and report your work. Each student will in turn be responsible for managing the group’s activities for a particular sub-project. Written formative feedback will be given at least twice throughout the semester. Average of 3 hours per week for weeks 2 to 10 inclusive. Learning outcomes 1, 2 and 3 A detailed work diary model and a proforma for expected content are provided in the Microbiology Methods Manual. In essence, the work diary will be evaluated against the criterion that a microbiologist unfamiliar with the project could read the diary and understand what had been done, why it was done and the logic of the conclusions drawn from the data. 16/05/08 at the conclusion of the Practical class. 10 Assessment task 7: Scientific report on sub-project 3 Scientific report evaluating methods and findings of sub-project 2. Report introduction, hypothesis, methods used, data obtained and a discussion addressing questions raised in project description (see Microbiology Laboratory Guide). Report should be typed. No more than 1000 words in total. Aim to be concise. Learning outcomes 1, 2 and 3 Task description Task length Links to unit’s learning outcomes Assessment criteria / guidelines Correct format of scientific report (see Student handbook). Experiments address hypothesis and discussion evaluates data appropriately. Consideration given to questions raised in Lab. Guide. 21/05/08 during tutorial Date due Assessment task 8: Online revision test of cell structure Log on through MyLO and complete the online revision test Task description number 3. Two attempts are allowed and the higher score recorded. 20 minutes Task length Learning outcomes 4a and d Links to unit’s learning outcomes Correct answers to questions. Assessment criteria / guidelines 21/06/08 at 12 am Date due Final exam Links to learning outcomes Description / conditions Date Learning outcomes 1 and 4a, b, c and d. The final exam will assess students’ ability to think scientifically in the context of microbiology. Two sections: one hour closed book multiple choice and 2-h written exam of essays, data analysis and short answer questions. All theory and practical aspects of the course are examinable. The final exam is conducted by the University Registrar in the formal examination period. See the Current Students homepage (>Examinations and Results) on the University’s website. How your final result is determined Continuous assessment Practical Schedule Group laboratory diary Project reports Taxonomic description Midsemester test Online revision test 5% 5% 20% 10% 10% 5% Exam Final exam 45% Successful performance in both the continuous assessment and final examination components is necessary for a pass in this unit. Irrespective of the aggregate mark, a student who fails to achieve at least 45% in each of the continuous assessment and the examination components may be required either to undertake further assessment, or be deemed to have failed the unit. In the latter case a grade, but no mark will be recorded for the unit. Submission of assignments Completed assignments are to be submitted electronically via MyLO – by the due date. Late assignments submitted electronically will lose 10% of the total mark for each day late. If 11 electronic submission fails, then hard copies may be submitted to the lecturer or to the secretary on the third floor. Requests for extensions All requests must be made directly to the lecturer well before the due date. It is expected that only unforeseeable circumstances, not poor organisation of work schedules, will be accepted as a reason for an extension. Penalties Unless granted an extension, a penalty of 10% of the total possible mark will be incurred for each day an assignment is late. Review of results and appeals In the first instance, the unit coordinator should be approached. If the issue is unresolved, then it should be referred to the Head of the School of Aquaculture. If this does not satisfactorily resolve the problem, then the appeal should be taken to the Faculty of Science Engineering and Technology. Academic referencing In your written work you will need to support your ideas by referring to scholarly literature, works of art and/or inventions. It is important that you understand how to correctly refer to the work of others so that you maintain your academic integrity. The School of Aquaculture student handbook contains advice on how to avoid plagiarism. Failure to appropriately acknowledge the ideas of others constitutes academic dishonesty (plagiarism), a matter considered by the University of Tasmania as a serious offence. The appropriate referencing style for this unit is based on the journal Aquaculture. A detailed description of the required style can be downloaded from the school’s website at: http://www.scieng.utas.edu.au/aqua/index.asp For general information on presentation of assignments, including referencing styles: http://www.utas.edu.au/library/assist/gpoa/gpoa.html Please read the following statement by the University on plagiarism. Should you require clarification please see your unit coordinator or lecturer. A tutorial session will be used to guide students on how to avoid plagiarism. 12 Plagiarism Plagiarism is a form of cheating. It is taking and using someone else's thoughts, writings or inventions and representing them as your own; for example, using an author's words without putting them in quotation marks and citing the source, using an author's ideas without proper acknowledgment and citation, copying another student's work. If you have any doubts about how to refer to the work of others in your assignments, please consult your lecturer or tutor for relevant referencing guidelines, and the academic integrity resources on the web at http://www.utas.edu.au/tl/supporting/academicintegrity/index.html. The intentional copying of someone else’s work as one’s own is a serious offence punishable by penalties that may range from a fine or deduction/cancellation of marks and, in the most serious of cases, to exclusion from a unit, a course or the University. Details of penalties that can be imposed are available in the Ordinance of Student Discipline – Part 3 Academic Misconduct, see http://www.utas.edu.au/universitycouncil/legislation/ The University reserves the right to submit assignments to plagiarism detection software, and might then retain a copy of the assignment on its database for the purpose of future plagiarism checking. For further information on this statement and general referencing guidelines, see http://www.utas.edu.au/plagiarism/ or follow the link under ‘Policy, Procedures and Feedback’ on the Current Students homepage. Withdrawing from a unit If you wish to withdraw from a unit, please be aware of the implications of withdrawing after the census date. Semester 1 From date End date 1 January 2008 21 March 2008 15 April 2008 7 June 2008 20 March 2008 14 April 2008 6 June 2008 Withdrawal code W0 WW Enrolment status Withdrawn Academic penalty NO Financial liability NO NO YES WN Withdrawn without penalty Withdrawn deemed failed YES YES AN Absent deemed failed YES YES 13