9th International Symposium on Toxicity Assessment 26 September to 1 October 1999 Pretoria, South Africa TECHNICAL PROGRAMMME SUNDAY, 26 SEPTEMBER WORKSHOP 09:30 - 10:00 Workshop Registration : Registration desk, In Touch Convention Centre 10:00 - 11:00 Group A : Toxicity testing of aqueous samples with the freshwater cnidarian Hydra attenuata: theoretical and practical aspects - Christian Blaise, Canada Group B : (a) Toxicity testing with Vibrio fisheri photobacteria. Traditional method and a new approach for solid and coloured samples - Juha Lappalainen, Finland; (b) Heavy metal biosensors - Marko Virta, Finland 11:00 - 11:15 Refreshments : Sponsored by OEN Enterprises (Pty) Ltd 11:15 - 12:15 Group A : (a) Toxicity testing with Vibrio fisheri photobacteria. Traditional method and a new approach for solid and coloured samples - Juha Lappalainen, Finland; (b) Heavy metal biosensors - Marko Virta, Finland Group B : Toxicity testing of aqueous samples with the freshwater cnidarian Hydra attenuata: theoretical and practical aspects - Christian Blaise, Canada 12:15 - 13:30 Lunch break 13:30 - 14:30 Group A : Use and application of the Biosensor, an automated and real-time water quality monitoring system - David Gruber, USA Group B : Fluoresence emission of algae as response to photosynthetic activity under the influence of toxic components in water samples - Christian Moldaenke, Germany 14:30 - 14:45 Refreshments - Sponsored by OEN Enterprises (Pty) Ltd 14:45 - 15:45 Group A : Fluoresence emission of algae as response to photosynthetic activity under the influence of toxic components in water samples - Christian Moldaenke, Germany Group B : Use and application of the Biosensor, an automated and real-time water quality monitoring system - David Gruber, USA 14:00 - 18:00 Symposium Registration : Foyer, Holiday Inn Garden Court 18:30 Meet and Greet : Rietvlei Dam Lapa i MONDAY, 27 SEPTEMBER 08:00 - 10:00 Registration continues : Registration Desk, In Touch Convention Centre OPENING SESSION CHAIRPERSON : Laetitia Slabbert, South Africa 09:00 - 09:05 Welcome - Ms Laetitia Slabbert, Chairman of the Organising Committee 09:05 - 09:15 Welcome address on behalf of the City of Pretoria - Dr Manie van Dyk, Acting Metro Mayor of Greater Pretoria 09:15 - 09:25 Welcome address on behalf of CSIR - Dr Anthos Yannakou, Director of Environmentek, CSIR 09:25 - 09:55 Opening address - Water Wars: The Potential of Water to Cause Conflict - Dr Peter Ashton, Divisional Fellow and Water Resources Specialist, Environmentek, CSIR 09:55 - 10:05 Address - Mr Piet Odendaal, President of the International Water Association (IWA) and Executive Director, Water Research Commission 10:05 - 10:10 10:10 - 10:30 Acknowledgement and vote of thanks to sponsors - Ms Laetitia Slabbert Tea/Coffee Briefing for chairpersons and presenters of Session 1 : Auditorium SESSION 1 : LABORATORY TECHNIQUES AND TEST KITS CHAIRPERSONS : Christian Blaise (Canada) and Takashi Kusui (Japan) 10:30 - 10:50 10:50 - 11:10 11:10 - 11:30 11:30 - 11:50 11:50 - 12:10 12:10 - 12:30 Toxkit microbiotests: New low cost tools for hazard detection/monitoring in environmental toxicology - G Persoone, Belgium Mammalian cell lines as a tool for toxicity assessment - J M Whitcutt and J J Alexander, South Africa Use of charophyte cell membrane reactions for toxicity assessment: In vivo and in vitro approaches - L Manusadzianas, G Maksimov, S Jurkonienë, K Sadauskas and R Vitkus, Lithuania A new method for measuring the toxicity of solid and colored samples with Vibrio fischeri J O Lappalainen, R Juvonen, K Vaajasaari and M T Karp, Finland In vitro urease enzyme test for water quality testing - E A Venter, J L Slabbert and N C Metelerkamp, South Africa Luminescence-based biosensors for the detection of bioavailable heavy metals - M P J Virta, J O Lappalainen, S Tauriainen, K Hakkila and M T Karp, Finland ii 12:30 - 13:40 13:20 - 13:40 Lunch Briefing for chairpersons and presenters of Session 2 : Auditorium SESSION 2 : ESTROGENIC EFFECTS CHAIRPERSONS : Guido Persoone (Belgium) and Alicia Ronco (Argentina) 13:40 - 14:00 14:00 - 14:20 14:20 - 14:40 14:40 - 15:00 15:00 - 15:20 15:20 - 15:40 On the measurement conditions for estrogen detection using yeast and estrogen screening of river water around industrial wastes disposal sites - I Aoyama, R Luo, X F Zhang and Y Shen, Japan The occurrence of estrogenic effects in Flanders, Belgium: a screening of resident fish populations - W M de Coen, K Van Campenhout, G Vendenbergh, B Denayer, C R Janssen and J P Giesy, USA Evaluation of estrogenic activity of environmental water by yeast screen - T Kusui and E Sugisawa, Japan The monitoring of water quality using a whole blood culture assay: The inflammatory activity of riverine and drinking water - D J Pool, J H van Wyk and A J Leslie Biomarkers for effects on reproduction and endocrine disruptors - B Sundelin, C Ryk and A-K Eriksson, Sweden Tea/Coffee Briefing for chairperson and presenters of Session 3 : Auditorium SESSION 3 : GENOTOXICITY AND MUTAGENICITY CHAIRPERSON : Arthur Barton (Australia) 15:40 - 16:00 16:00 - 16:20 Development of a test strategy for the detection of genotoxic effects in surface waters - T Grummt, Germany Somatic cell mutation induced by sunlight and UV-irradiation in Drosophila - T Negishi, M Toyoshima, S Takinami, M Mochizuki, H Hayatsu, O Nikaido, M Kubota, M Watanabe, M Hieda and K Hieda, Japan 18:30 - 20:00 Cocktail Reception - In Touch Convention Centre TUESDAY, 28 SEPTEMBER 08:00 - 08:30 Briefing for chairperson and presenters of Session 4 : Auditorium SESSION 4 : WATERTOX NETWORK CHAIRPERSON : Gilles Forget, Canada 08:30 - 08:35 08:35 - 08:50 08:50 - 09:05 09:05 - 09:20 09:20 - 09:35 09:35 - 09:50 09:50 - 10:10 Welcome - G Forget, Canada Overview of results of the eight country IDRC Watertox Project - G Forget, A Sánchez and B J Dutka, Canada The use of a set of animal and plant bioassays to estimate toxicity of organic and inorganic substances - V V Arkhipchuk, V D Romanenko, M V Malinovskaya, L S Kipnis, V D Solomatina and Y G Krot, Ukraine Evaluation of a battery of toxicity tests for use in the assessment of water quality in a Costa Rican laboratory - L E Castillo, M Pinnock and E Martínez, Development and validation of a battery of bioassays - The Colombia experience - M C Diaz-Baez and J Perez, Colombia Assessment of a battery of simple bioassays for the determination of surface water quality by a Canadian community-based laboratory - J J Ridal and T Beauregard, Canada Questions iii 10:10 - 10:30 Tea/Coffee SESSION 4 : WATERTOX NETWORK continues CHAIRPERSON : Gilles Forget, Canada 10:30 - 10:45 12:00 - 12:15 12:15 - 12:30 Standardization of a battery of bioassays for water toxicity testing: Results of a study in Chile - G Castillo, Chile Toxicity assessment of water with a battery of bioassays - Results from México - M C Y Pica Granados, México A comparative study of selected bioassays to determine water toxicity (India) - R C Srivastava and D Datta, India Comparative response of a battery of toxicity tests for water quality assessment within an intercalibration network: Results from Argentina - A E Ronco, M C Sobrero, V I Grassi, L P Kaminski, G B Rossini and L A Massolo, Argentina Evaluation of three water concentration techniques as a previous step to test acute toxicity - M C Diaz-Baez, L E Cruz, J Perez, D Rodriguez Toxicity screening of aqueous samples using a cost-effective 72-h exposure Selenastrum capricornutum assay - C Blaise, G Forget and S Trottier, Canada Questions Conclusions 12:30 - 13:40 13:20 - 13:40 Lunch Briefing for chairpersons and presenters of Session 5 : Auditorium 10:45 - 11:00 11:00 - 11:15 11:15 - 11:30 11:30 - 11:45 11:45 - 12:00 SESSION 5 : BIODEGRADATION AND TOXICITY CHAIRPERSONS : Isao Aoyama (Japan) and Lesley Phillips (South Africa) 13:40 - 14:00 14:00 - 14:20 14:20 - 14:40 14:40 - 15:00 15:00 - 15:20 15:20 - 15:40 The effects of early diagenesis on [13C] pyrene fate in sediments - E A Guthrie, J M Bortiatynski, J E Richman, B Chefetz, J D H Van Heemst and P G Hatcher, USA Microbial metabolism of chlorothalonil residues in agricultural soil - M van Eeden, H C Potgieter, H A Esterhuysen and A M van der Walt, South Africa Factors affecting the rate of biodegradation - D Liu, R J Maguire, Y L Lau, G J Pacepavicius, H Okamura and I Aoyama, Canada The impact of herbicides on the biological activity, measured with the Bait-Lamina test - A field study in a vineyard in the Western Cape - B Helling, A J Reinecke, K Louw, J Fourie and S A Reinecke, South Africa The toxicity and biodegradability of eight main phenolic compounds of the oil-shale industry wastewaters: a test battery approach - A Kahru, L Pollumaa, R Reiman, A Rätsep and A Maloveryan, Estonia Tea/Coffee Briefing for chairperson and presenters of Session 6 : Auditorium SESSION 6 : REGULATORY USE OF TOXICITY TESTS CHAIRPERSON : Rick Scroggins (Canada) 15:40 - 16:00 16:00 - 16:20 The US Clean Water Act and the application of microbiotests to demonstrate compliance - D Gruber, USA Use of toxicity testing for effluent assessment and control in Canadian Regulatory Programs - R P Scroggins, Canada 16:20 - 17:20 Open Forum Discussion Panel: David Gruber (USA), Guido Persoone (Belgium), Hein du Preez (South Africa), Malcolm McCormick (Australia), Isao Aoyama (Japan), and Alicia Ronco (Argentina) iv WEDNESDAY, 29 SEPTEMBER 08:00 - 22:00 Field trip THURSDAY, 30 SEPTEMBER 08:10 - 08:30 Briefing for chairpersons and presenters of Session 7 : Auditorium SESSION 7 : APPLICATION: GROUND-, SURFACE AND DRINKING WATER CHAIRPERSONS : Anneli Kühn (South Africa) and Dickson Liu (Canada) 08:30 - 08:50 09:50 - 10:10 Evaluation of groundwater toxicity using bioassay, chemical and TIE analyses - K E Gustavson and J M Harkin, USA Use of specific sorbents and rapid bioassays for groundwater monitoring - K E Gustavson and J M Harkin, USA Evaluation of toxicity assays for non-aqueous phase liquid (NAPL) detection in groundwater - J L Slabbert, E A Venter, E Pienaar, R Meyer and C Ellerbeck, South Africa Heavy metal binding capacity of environmental samples, using MetPAD/MetPLATE - G Bitton, F Huang, I C Kong and J-L Morel, USA Toxicity testing at Goreangab Reclamation Plant - past, present, future - J G Menge and J L Slabbert, Namibia 10:10 - 10:30 Tea/Coffee 08:50 - 09:10 09:10 - 09:30 09:30 - 09:50 SESSION 8 : POSTERS CHAIRPERSON : Wim de Coen (Belgium) 10:30 - 11:30 11:30 - 12:30 Poster Viewing : Atrium Poster Discussion : Auditorium Discussion Panel: Gabriel Bitton (USA), Christian Blaise (Canada), Falk Krebs (Germany) and Kristin Becker van Slooten (Switzerland) 12:30 - 13:40 13:20 - 13:40 Lunch Briefing for chairpersons and presenters of Session 9 : Auditorium SESSION 9 : SEDIMENT AND SOIL TOXICITY CHAIRPERSONS : Colin Janssens (Belgium) and Malcolm McCormick (Australia) 13:40 - 14:00 14:00 - 14:20 14:20 - 14:40 14:40 - 15:00 15:00 - 15:20 Use of bioassays to develop a PAH cleanup strategy for Hamilton Harbour - T Murphy, Canada Genotoxicity and estrogenicity of substances extracted from road sediments - Y Ono, H Nagadome, E Degirmenci, S Kanoh and O Kawara, Japan Acute and chronic toxicity of estuarine sediments to the Manila clam, Tapes semidecussatus - P A Byrne and J O'Halloran, Ireland The dynamics of metals and hydrophobic organic contaminants in sediments of euxinic bottoms - A-K Eriksson, B Sundelin and E Håkansson, Sweden Development of a standardized reference sediment to improve the usefulness of marine benthic toxicity testing as a regulatory tool - I Still, S Rabke and J Candler v 15:20 - 15:40 Tea/Coffee Briefing for chairpersons and presenters of Session 10 : Auditorium SESSION 10 : BIOMARKERS CHAIRPERSONS : Gabriel Bitton (USA) and Annette Venter (South Africa) 15:40 - 16:00 16:00 - 16:20 16:20 - 16:50 16:50 - 17:30 19:30 Molluscan shellfish biomarker study of the Saguenay Fjord, Québec, Canada - C Blaise, F Gagné, J Pellerin and P-D Hansen, Canada Effect of a herbicide on biomarkers and life cycle parameters of the collembola Folsomia candida - C Stämfli, K Becker van Slooten and J Tarradellas, Switzerland Heat shock protein expression as a biomarker for detection of environmental stress in sea bream - N Y S Woo, E E Deane, S P Kelly and C K M Lo, China Preview of the 10th International Symposium on Toxicity Assessment to be held in Quebec, Canada, 2001 - Lynn Cleary, Canada Banquet - Stars Studio FRIDAY, 1 OCTOBER 08:10 - 08:30 Briefing for chairpersons and presenters of Session 11 : Auditorium SESSION 11 : ON-LINE MONITORING, AND LABORATORY AND FIELD ASSAYS CHAIRPERSONS : John Harkin (USA) and Anders Svenson (Sweden) 08:30 - 08:50 08:50 - 09:10 09:10 - 09:30 09:30 - 09:50 09:50 - 10:10 10:10 - 10:30 Automated biological monitors as bio-markers - D Gruber and W J Rasnake, USA Monitoring of surface waters by ultra-sensitive Daphnia Toximeter - C Moldaenke, Germany MetPlant: A test for detecting metal-accumulating plants growing on contaminated soils G Bitton, A Boularbah, J-L Morel and C Schwartz, USA Comparison of community changes and tolerance analyses for the assessment of wastewater ecotoxicity - D Soldo and R Behra, Switzerland Development of marine sediment re-worker toxicity tests with Western Australian species - Y B Tsvetnenko, A J Black and L H Evans, Australia Tea/Coffee Briefing for chairpersons and presenters of Session 12 : Auditorium SESSION 12 : EFFLUENT TOXICITY AND DATA EVALUATION CHAIRPERSONS : Gabriela Castillo (Chile) and Barney Dutka (Canada) 10:30 - 10:50 10:50 - 11:10 11:10 - 11:30 11:30 - 11:50 11:50 - 12:10 12:10 - 12:30 Experiences with testing of chemicals used in the oil and gas industry in Western Australia - A P Barton, Australia Effects of copper and electroplating effluent on the filtration rate of the green mussel Perna viridis - C K Wong and P K Wong, China Argentina: The need of developing toxicity tests to assess the efficiency of treatment removal for industrial effluents and water quality of receiving waters - C E Gomez, L L Contento and A Carsen, Argentina Wastewater toxicity screening with microbiotests in a Northern Italy joint activated sludge plant - M Cardinaletti, Italy Agricultural pesticide contamination in the Lourens River, Western Cape: Importance of spraydrift and runoff for a field-relevant exposure assessment - R Schulz, J M Dabrowski, C Hugo, V Krause, S K C Peall and A J Reinecke, South Africa The use of principal components analysis to analyse toxicity data - C Blaise, D J Kew, T vi Kusui, M J McCormick and R J McCormick, Australia 12:30 - 13:40 13:20 - 13:40 Lunch Briefing for chairpersons and presenters of Session 13 : Auditorium SESSION 13 : ALGAL AND FUNGAL TOXINS CHAIRPERSONS : Hein du Preez (South Africa) and Ian Falconer (Australia) 13:40 - 14:00 14:00 - 14:20 14:20 - 14:40 14:40 - 15:00 15:00 - 15:20 15:20 - 15:40 Detection of cyanobacterial toxicity - I R Falconer, Australia Incorporation of the ELISA mycrocystin rapid screening kit in Rand Water's cyanophyceae toxin monitoring protocol - H Mulder, Z C Ngwenya, L Grobler and H H du Preez, South Africa Tests for the toxicity assessment of cyanobacterial bloom samples - M Tarczynska, G Nalecz-Jawecki, J Sawicki, K A Beattie, G A Codd and M Zalewski, Poland Cyanobacterial toxins showing pesticidal and mosquito larvicidal activity - A Kumar, D P Singh and M B Tyagi, India Toxicity assessment of fungal cultures and mycotoxins - G S Shephard, W F O Marasas and W C A Gelderblom, South Africa The occurrence of fumonisin B1 in Egyptian-market corn: Screening, processing and toxicology - A M Aboul-Enein, H A Amra, A A Ragab and M I Mohamed, Egypt 15:40 - 16:00 Closure and Presentation of Awards - Laetitia Slabbert 16:00 - 16:20 Tea/Coffee vii POSTER PRESENTATIONS BIOASSAY TECHNIQUES 1 Evaluation of a ß-galactosidase in vitro enzymatic test specific for heavy metal toxicity - C D Apartin and A E Ronco, Argentina 2 Tetrahymena pyriformis - a test system for environmental medicine - A Mueller, O Herbarth and A E Ronco, Germany BIOMARKERS 3 Biomarkers of freshwater ecotoxicity: Recovery capacity of fish under field cage conditions - F R de la Torre, L Ferrari and A Salibián, Argentina 4 Recovery capacity of fish biomarkers from a heavily polluted river under laboratory conditions - F R de la Torre, L Ferrari and A Salibián, Argentina 5 Fish biomarkers as toxicity assessment tools in simultaneous analysis in two sites of a polluted river - F R de la Torre, A Salibián and L Ferrari, Argentina 6 Using a mesocosm approach to assess the aquatic health of an urban and an agricultural stream - T M Tuominen, M Sekela, S Sylvestre and G Moyle, Canada 7 Biomarker assays for the detection of chronic toxicity in the aquatic environment - A Vorster and J H J van Vuren, South Africa 8 Effect of Cr(VI) on the nucleolar activity in liver cells from Odontesthes bonariensis (Pisces) - P Carriquiriborde, F Dulout and A E Ronco, Argentina 9 Hormesis: A stress response in cells exposed to low levels of toxic agents - L H Damelin, S Vokes, J M Whitcutt, S B Damelin and J J Alexander, South Africa 10 Preliminary evaluation of biomarkers for the detection of chronic toxicity in Oreochromis mossambicus - A Joubert, E A Venter, J L Slabbert, A Vorster, J H J van Vuren and L P D de Wet, South Africa 11 A short term Hydra attenuata toxicity test based on enzymatic inhibition - R Luo, F Gagné, C Blaise and I Aoyama, Japan 12 Toxicological studies on diazinon: Biochemical effects of residues on fish muscles - H N El-Khatib and S A A Shokr, Egypt 13 Stress protein expression in the freshwater bivalves Limnoperna fortunei and Corbicula fluminea exposed to heavy metals - C Oliver, A Porta and A E Ronco, Argentina 14 An enzyme linked immunosorbent assay (ELISA) for measuring vitellogenin, a biomarker for xenobiotic estrogens - E J Pool, J H van Wyk and A J Leslie, South Africa 15 Biomarkers in aquatic plants. Heavy metal uptake and changes of electrophoretic protein patterns in Pistia stratiotes, Sagitaria montevidensis and Schoenoplectus californicus - S Ramírez, A Porta, N Caffini and A E Ronco, Argentina viii MUTAGENICITY 16 Desmutagenic and/or antimutagenic activity of soil humic substances in germinating seeds of Vicia faba - G Ferrara, E Loffredo, R Simeone and N Senesi, Italy 17 Cytogenetic consequences of a toxic impact on cells of Allium cepa, Lactuca sativa and Hydra attenuata - V V Arkhipchuk, M V Malinovskaya and N N Garanko, Ukraine ON-LINE MONITORING 18 Biological early warning systems in drinking water production - E J M Penders and P G Stoks, The Netherlands ALGAL TOXINS 19 Algal toxin initiation of avian botulism? - T Murphy, K Irvine, C Nalewajko, H Murkin and L Ross, Canada 20 Microcystis aeruginosa in the Lake Velencei - M N Reskóné and A K Törökné, Hungary 21 Cyanobacterial toxins detected by Thamnotox kitFM. (A double blind experiment) - A K Törökné and K Sivonen, Hungary BIODEGRADATION AND TOXICITY 22 Removal of a Sasol surfactant in surface water using a modified "stair-case" river model - B Goronyane, South Africa 23 Fate and ecotoxicity of the new antifouling compound Irgarol 1051 in the aquatic environment - H Okamura, I Aoyama, D Liu, R J Maguire, G J Pacepavicius and Y L Lau, Japan CHEMICAL TECHNIQUES 24 A sensitive analysis of aldehydes in environmental samples - T Horike, M Iden, T Hidehira and S Kira, Japan 25 Measurement of benzo(a)pyrene in sea water and in mussels in the Seto Inland Sea, Japan - S Kira, A Seki, S Nakayama, T Katsuse, Y Nogami and T Ito, Japan 26 Benzo(a)pyrene adsorbed to suspended solid in fresh water - Y Nogami, R Imaeda, T Ito and S Kira, Japan 27 HEX-MS: Investigation on the use of an MCN and a Meinhard nebuliser - C Thomas, F AbouShakra, A Eaton, D Churchman and K Compson, UK CHEMICAL EFFECTS 28 Influence of storage conditions in the acute toxicity to aquatic biota of water samples fortified with pesticides - M J Cerejeira, T Pereira and J Espiríto-Santo, Portugal 29 Use of microbiotests to compare the toxicity of water samples fortified with active ingredient and formulated pesticides - T Pereira, M J Cerejeira and J Espiríto-Santo, Portugal 30 The use of microbiotests, Daphnia magna reproduction test and some "rapid" methods in the assessment of pesticides toxicity - P Fochtman, Poland 31 Effect of Cr(VI) on early life stages of three species of hylid frogs (Amphibia, Anura) from South ix America - G Natale, N Basso and A E Ronco, Argentina 32 Organotin compounds on Portuguese coastal waters - Sado and Mira estuaries case studies - M F Pessoa, A Fernando and J S Oliveira, Portugal SEDIMENT TOXICITY 33 Toxicity tests on sediment from the Saigon river and Hochiminh City urban waterways, Vietnam L C Do Hong, K Becker Van Slooten, J-J Sauvain and J Tarradellas, Vietnam 34 Sediment bioassessment with a battery of assays and sediment phases: pore water, elutriates and extracts - F Krebs, Germany APPLICATION: DRINKING WATER, SURFACE WATER AND EFFLUENTS 35 Feasibility study on incorporating the Aquanox field instrument in Rand Water's biomonitoring programme - H Mulder, D Masoabi and H H du Preez, South Africa 36 The perspective of microbiotests application in water monitoring system in Estonia - I Blinova, Estonia 37 Microbiotests in Portugal - a target for the next years - M A Morbey and M F Brito, Portugal 38 Effect of sewage effluents on phytobenthos - M Husain and A M Fahmi, Kuwait 39 Biomarker assays for ecotoxicity assessment from heavy metals and wastewaters - G Castillo, I Vila, and E Neild, Chile 40 Toxicity identification and evaluation of effluent from a high density sludge plant on an East Rand gold mine - L P D de Wet, J L Slabbert, R D Walmsley and H Oelofse, South Africa 41 Toxicity identification evaluation of nitrification inhibitors in wastewaters - A Svenson, B Sandén, G Dalhammar, M Remberger and L Kaj, Sweden x