國立嘉義大學九十八學年度
食品科學系碩士班（保健食品組）招生考試試題
科目：生物化學
一、選擇題：(2% each)
1. Which of the following is a palindromic sequence?
(A) AGGTCC
(B) CCTTCC
(C) GAATCC
(D) GGATCC
TCCAGG
GCAAGG
CTTAGG
CCTAGG
2. The anaerobic conversion of 1 mol of glucose to 2 mol of lactate by fermentation is accompanied by a net
gain of:
(A) 1 mol of ATP.
(B) 1 mol of NADH.
(C) 2 mol of ATP.
(D) 2 mol of NADH.
3. An enzyme used in both glycolysis and gluconeogenesis is:
(A) 3-phosphoglycerate kinase. (B) glucose 6-phosphatase. (C) hexokinase. (D) phosphofructokinase-1.
4. The oxidation of 3 mol of glucose by the pentose phosphate pathway may result in the production of:
(A) 2 mol of pentose, 4 mol of NADPH, and 8 mol of CO 2.
(B) 3 mol of pentose, 4 mol of NADPH, and 3 mol of CO2.
(C) 3 mol of pentose, 6 mol of NADPH, and 3 mol of CO2.
(D) 4 mol of pentose, 3 mol of NADPH, and 3 mol of CO2.
5. Which of the following statements is true of muscle glycogen phosphorylase?
(A) It catalyzes phosphorolysis of the(1  6)bonds at the branch points of glycogen.
(B) It catalyzes the degradation of glycogen by hydrolysis of glycosidic bonds.
(C) It degrades glycogen to form glucose 6-phosphate.
(D) It exists in an active (a) form and an inactive (b) form that is allosterically regulated by AMP.
6. Which of the below is not required for the oxidative decarboxylation of pyruvate to form acetyl-CoA?
(A) ATP
(B) CoA-SH
(C) FAD
(D) Lipoic acid
7. Saturated fatty acids are degraded by the stepwise reactions of oxidation, producing acetyl-CoA. Under
aerobic conditions, how many ATP molecules would be produced as a consequence of removal of each
acetyl-CoA?
(A) 2
(B) 3
(C) 4
(D) 5
8. If an aerobic organism (for example, the bacterium E. coli) were fed each of the following four compounds
as a source of energy, the energy yield per mole from these molecules would be in the order:
(A) glucose > alanine > palmitate. (B) glucose > palmitate > alanine.
(C) palmitate > alanine > glucose. (D) palmitate > glucose > alanine.
9. The synthesis of both glycerophospholipids and triacylglycerols involves:
(A) CDP-choline. (B) CDP-diacylglycerol. (C) phosphatidate phosphatase. (D) phosphatidic acid.
10. For amino acids with neutral R groups, at any pH below the pI of the amino acid, the population of amino
acids in solution will have:
(A) a net negative charge. (B) a net positive charge.
(C) no charged groups. (D) no net charge.
二、簡答題：
1. The composition (mole fraction) of one of the strands of a double-helical DNA is [A] = 0.3, and [G] = 0.24.
Calculate the following, if possible. If impossible, write "I." (4%)
For the same strand:
For the other strand:
[T] = ____
[A] + [T] = ____
[T] + [C] = ____
[C] = ____
2. (1) Predict the fragments that will be generated from the treatment of the following peptide with trypsin. (3%)
Gly-Ala-Trp-Arg-Asp-Lys-Glu-Phe-Gly-Gln
(2) For the following set of components, determine the final electron acceptor. Assume O2 is present. (3%)
NADH, Q, complexes, I, III, and IV
3. An enzyme follows Michaelis-Menten kinetics. Indicate (with an "x") which of the kinetic parameters at the
left would be altered by the following factors. Give only one answer for each. (4%)
Km
Vmax
Neither
Both
(a) a competitive inhibitor
(b) a mixed inhibitor
(c) 6 M urea
(d) doubling [S]
4. (1) What is the effect of pH on the binding of oxygen to hemoglobin (the Bohr Effect) (4%)
(2) If brewer’s yeast is mixed with pure sugar (glucose) in the absence of phosphate (P i), no ethanol is
produced. With the addition of a little Pi, ethanol production soon begins. Explain this observation. (4%)
(3) The citric acid cycle begins with the condensation of acetyl-CoA with oxaloacetate. Describe three
possible sources for the acetyl-CoA. (4%)
(4) 下列短 peptide 預測其二級結構三級結構 (α-helix; random coil; β-sheet; disulfide bond; ββ
motif; ααmotif) (4%)
GLy-Ala-Ala-Gly-Ser-Gly-Ala-Pro-Ala-Gly-Ala-Ala-Ser-Tyr-Gly
5. (1) Describe the physiological significance of 2,3-Bisphosphoglycerate (2,3-BPG). (5%)
(2) How does prokaryotic DNA replication appear to differ from eukaryotic DNA replication?(5%)
三、問答題：
1. Describe the metabolic role of following terms in biochemical reactions: (10%)
(1) Carnitine (2) Creatine (3) Ketone body (4) Glutathione (5) Arachidonic acid
2. Describe the significance of following terms in metabolism: (10%)
(1) Pyruvate/Malate cycle (2) Malate/Aspartate shuttle (3) The Cori cycle
3. What are the two types of enzyme inhibitors? Give an example of each. (10%)
4. Give an example of each of the following: (10%)
(1) Reducing sugar (2) Glycosidic linkage (3) Polyunsaturated fatty acid
(4) Epimer
(5) Acid-conjugate base pair