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Thermo Scientific
Molecular Biology Marketplace
A quarterly publication containing special offers
for significant savings on a variety of molecular
biology products.
p2 Molecular Biology Tools
p5 PCR and qPCR
2
Thermo Scientific Molecular Biology Tools
Thermo Scientific GeneJET Nucleic Acid
Purification Kits – 30% Discount!
Nucleic Acid
Purification
Plasmid
DNA
Miniprep
30% OFF
Midiprep Maxiprep
Genomic
DNA
Plant
DNA
Blood
DNA
DNA
Fragments
Total RNA
DNA from
Cells &
Tissues
Plant
RNA
Blood
RNA
RNA from
Cells &
Tissues
From PCR From Gels
30% Discount on NEW GeneJET TM Kits
GeneJET Plasmid Maxiprep Kit
GeneJET Plasmid Midiprep Kit
Centrifuge/Vacuum Protocol
Pelleted bacteria
Pelleted bacteria
High quality plasmid DNA: A260/280 1.7 – 1.9
High yields and high concentration of purified DNA
• Maxiprep – 750 μg, ≥ 400 ng/μL
• Midiprep – 200 μg, ≥ 400 ng/μL
Silica-based membrane technology
Centrifuge and vacuum manifold protocols
Purified DNA is ideal for transfection of robust cell lines
Product
Preps
Alkaline lysate
Alkaline
lysate
Clear lysate by
centrifugation
Clear lysate by
centrifugation
Bind DNA
Bind
DNA
Cat. No.
List Price
30% Off
GeneJET Plasmid Maxiprep Kit
25
10
K0492
K0491
$475
$218
$333
$153
GeneJET Plasmid Midiprep Kit
100
25
K0482
K0481
$820
$230
$574
$161
Please quote promotion code MP33 and refer to the back cover for ordering details.
Offers are valid until March 31st, 2012.
Wash
Wash
Elute
Elute
GeneJET Whole Blood Genomic DNA Purification Mini Kit
GeneJET Whole Blood RNA Purification Mini Kit
GeneJET Whole Blood Genomic DNA Mini Purification Kit
• Purify RNA-free genomic DNA
• Fast 20-minute procedure with no separate cell lysis step
GeneJET Whole Blood RNA Purification Mini Kit
• Purify DNA-free RNA with no DNase step
• High RNA Integrity (RIN > 8)
Spin columns based on silica-membrane technology
Specialized and detailed protocols for different sample types
Purified DNA & RNA can be used in a wide range of downstream applications
Product
GeneJET Whole Blood Genomic
DNA Purification Mini Kit
GeneJET Whole Blood RNA
Purification Mini Kit
Offers are valid until March 31, 2012.
Preps
Cat. No.
List Price
30% Off
250
50
K0782
K0781
$500
$125
$350
$88
50
K0761
$250
$175
Compatible with a wide
variety of samples:
• Fresh or frozen
• Anticoagulants: EDTA,
citrate, heparin
• Buffy coat
• Bone marrow
• Body fluids (urine)
• Multiple animal species
3
Thermo Scientific Molecular Biology Tools
30% OFF
GeneJET Plant Genomic DNA Purification Kit
GeneJET Plant RNA Purification Kit
Compatible with a wide variety
of samples:
• Multiple plant species
GeneJET Plant Genomic DNA Purification Kit
• Purify DNA without column-clogging lysate filtration steps
• Material-specific protocols provided
GeneJET Plant RNA Purification Kit
• Purify DNA-free RNA with no DNase step
• High RNA Integrity (RIN > 8)
Product
Preps
• Various plant tissues:
• Leaves, roots, seeds, pine needles, etc.
Cat. No.
List Price
30% Off
GeneJET Plant Genomic DNA Purification Kit
250
50
K0792
K0791
$625
$156
$438
$109
GeneJET Plant RNA Purification Kit
250
50
K0802
K0801
$1000
$250
$700
$175
Offers are valid until March 31, 2012.
30% Discount on our Most Popular GeneJET Kits
30% OFF GeneJET Plasmid Miniprep Kit
High quality plasmid DNA: A260/280 1.7 – 1.9
High yields and high concentration of purified DNA: 20 μg ≥ 400 ng/μL
GeneJET PCR Purification Kit
GeneJET Gel Extraction Kit
Consistent and Reproducible Miniprep Yields
GeneJET PCR Purification Kit
• DNA recovery up to 100% (25 bp – 20 kb)
• Effective removal of primers, unincorporated nucleotides,
enzymes and salts
GeneJET Gel Extraction Kit
• DNA recovery up to 95% (25 bp – 20 kb)
• Purify DNA fragments from standard or low-melt agarose
gels run with TAE or TBE buffer.
GeneJET Genomic DNA Purification Kit
GeneJET RNA Purification Kit
GeneJET Genomic DNA Purification Kit
• Purify RNA-free gDNA from a wide variety of tissues or
cells in only 20 minutes
GeneJET RNA Purification Kit
• Purify high integrity (RIN > 8.0), DNA-free total RNA from
a wide variety of sources
Product
Preps
Relaxed
Supercoiled
M
1
2
3
4
5
6
A pBluescript-based high copy
number plasmid was purified
from E.coli culture with 6 different
GeneJET Spin Columns
M – Thermo Scientific FastRuler
DNA Ladder, High Range, readyto-use (#SM1123)
1-6 – plasmid DNA isolated with 6
different GeneJET Spin Columns
Cat. No.
List Price
30% Off
GeneJET Plasmid Miniprep Kit
250
50
K0503
K0502
$339
$83
$237
$58
GeneJET Gel Extraction Kit
250
50
K0692
K0691
$477
$106
$334
$74
GeneJET PCR Purification Kit
250
50
K0702
K0701
$477
$106
$334
$74
GeneJET Genomic DNA Purification Kit
250
50
K0722
K0721
$650
$146
$455
$102
GeneJET RNA Purification Kit
250
50
K0732
K0731
$1040
$265
$728
$185
Offers are valid until March 31, 2012.
www.thermoscientific.com/mpcda
4
Thermo Scientific Molecular Biology Tools
Upgrade to FastDigest Restriction Enzymes
®
30% OFF Experience the power and convenience of FastDigest Restriction enzymes
•
•
•
•
One universal buffer for all 176 FastDigest enzymes
Complete digestion in 5-15 minutes
Direct loading on gels with FastDigest Green Buffer
100% buffer compatibility with downstream applications
One universal buffer for all FastDigest enzymes
To view a list of available enzymes and our FastDigest
“The Great Double Digestion Day” video visit:
www.thermoscientific.com/fastdigest
Offers are valid until March 31, 2012.
Superior Transfection Efficiency With Minimal Toxicity
30% OFF Thermo Scientific TurboFect Transfection Reagent is the reagent of choice for shRNA and plasmid
transfection applications.
• High transfection efficiency of a wide variety of cell types, including primary, differentiated and
undifferentiated cells
• Transfection can be performed in the presence or absence of serum
• Demonstrates superior transfection efficiency and minimal toxicity when compared to lipid-based or
other polymer-based transfection reagents
• Simple 3-step transfection protocol compared with 6-7 steps for other transfection reagents
Cat. No
Description
Quantity
List Price
30% Off
R0531
TurboFect Transfection Reagent
1.0 mL
$341
$239
Offers are valid until March 31, 2012.
1500
75
1250
1000
50
750
500
25
250
0
NTC TurboFect Lipofect- Fugene 6
amine 2000
From April 1, 2012 TurboFect™ Transfection
Reagent will replace ExGen 500, Arrest-In™ and
Express-In Transfection Reagents.
Orders for these discontinued products will
continue to be processed until March 31, 2012.
0
GFP Positive Cells (%)
Mean Fluorescence Intensity
GFP Positive Cells (%)
1750
HepG2 Cells
50
1500
40
1250
1000
30
750
20
500
10
0
250
NTC TurboFect Lipofect- Fugene 6
amine 2000
0
Transfection efficiency
Mean fluorescence intensity
60
1500
50
1250
40
1000
30
750
20
500
10
250
0
NTC TurboFect Lipofect- Fugene 6
amine 2000
0
TurboFect Reagent demonstrates higher transfection
efficiency and improved plasmid expression across cell lines
Cells were transfected with plasmid DNA encoding eGFP with
the indicated transfection reagent (NTC; non-treated control).
Transfections were performed according to manufacturers’
recommendations and subsequent GFP expression was analyzed
by flow cytometry.
Mean Fluorescence Intensity
WEHI Cells
2000
Mean Fluorescence Intensity
100
GFP Positive Cells (%)
HeLa Cells
Thermo Scientific PCR and qPCR
5
Take A More Direct Route To PCR
Thermo Scientific Direct PCR Kits
FREE SAMPLE
Get a free sample!
Request your
free kit at www.
thermoscientific.
com/
directpcrsample
Direct PCR saves you time and cost by allowing amplification of DNA directly from the sample
material. No DNA purification is needed. The Direct PCR approach is based on unique Thermo
Scientific Phusion and Phire DNA Polymerases which are extremely tolerant of PCR inhibitors
present in sample materials and guarantee high yields of specific PCR product.
• No need for time consuming and expensive DNA purification steps
• Simple and short protocols with minimal hands-on time
• Very little starting sample material required
• Control primers and detailed instructions for use included in each kit
• Optimized kits for plant, animal tissues, blood and human specimen
Cat. No
Description
Quantity
F-130X
Phire Plant Direct PCR Kit
20 rxns 50 µL each
F-140X
Phire Animal Tissue Direct PCR Kit
20 rxns 50 µL each
F-150X
Phusion Human Specimen Direct PCR Kit
20 rxns 20 µL each
F-547X
Phusion Blood Direct PCR Kit
20 rxns 20 µL each
Campaign is valid until March 31st, 2012.
Customer feedback on Phire Animal Tissue Direct PCR Kit:
The quality of PCR results was as good or better than with more time-consuming extraction
methods, followed by PCR (in terms of yield and clarity of bands on the agarose gel), so it allowed us
to streamline the species ID assay. Given the small amount of tissue required, it will also allow us to
refine our tissue collection methods so that we can take minute fin samples from live fish.
- Dr. Margaret Docker, Department of Biological Sciences, University of Manitoba, Canada
I used the Phire Animal Tissue Direct PCR Kit on the nematode C.elegans. I achieved good clean
product with results comparable to using extracted DNA. By side stepping the need to extract and
clean up the DNA we can save up to half a day using the Direct PCR kit, great when we are working
with a number of different strains.
- Laura Weldon, Technician, Nematode Biology Research, Bristol University, UK
Find out more information about Direct PCR at www.thermoscientific.com/directpcr
www.thermoscientific.com/mpcda
6
Thermo Scientific PCR and qPCR
qPCR Reagents and First Strand cDNA Synthesis Kits
For Fast Real-Time qPCR
Thermo Scientific DyNAmo Flash and ColorFlash qPCR kits for SYBR Green and probe chemistries optimized 2x master mixes for extremely fast qPCR protocols.
• The engineered Tbr DNA polymerase allows a combined annealing and extension
Time savings using fast qPCR reagents
step of only 15 seconds
120
• Specific and sensitive detection
• Included dUTP allows the use of UNG for prevention of carry-over contamination
100
• Blue master mix combined with yellow sample buffer turns the reaction mix green
80
that helps ensure correct pipetting (DyNAmo ColorFlash Kits)
Minutes
30% OFF
60
40
Thermo Scientific Maxima First Strand Synthesis Kits utilize Maxima Reverse
Transcriptase, an advanced enzyme derived from in vitro evolution of M-MuLV RT that
20
delivers faster cDNA synthesis than ever before.
0
• Increased synthesis rates - complete cDNA synthesis in 15 minutes
Standard qPCR Fast qPCR
instrument
instrument
• Sensitive and reproducible - cDNA synthesis from a wide range of total RNA
Reagent for standard cycling
G
C
G
A
T
A
T
C
G
A
T
C
G
A
T
C
G
A
T
C
G
A
T
C
G
A
T
C
G
A
T
C
G
A
amounts (1 pg - 5 μg)
Fast qPCR reagent
• Increased reaction temperatures
A T C G A T C -Gactive
A T C up
G AtoT65°C
CGATCGATCGGCGATATCG
A
• Increased reproducibility
reduced
bias
ATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGA
ATCGATCGATCGATCGATCGATCGATCGAGATCGCGATATCGATCGATCGCGA
Quantity
List Price 30% OFF
CGATCGATCGCGATATCGATCGATCGCGATATCGATCGATCGCGATATCGATC
F-416L
DyNAmo ColorFlash SYBR Green qPCR Kit
500 x 20 μL rxns
$428
$300
CGCGATATCGATCGATCGCGATATCGATCGATCGCGATATCGATCGATCGCGA
F-415L
DyNAmo Flash SYBR Green qPCR Kit
500 x 20 μL rxns
$428
$300
CGATCGATCGCGATATCGATCGATCGCGATATCGATCGATCGCGATATCGATC
F-456L
DyNAmo ColorFlash Probe qPCR Kit
500 x 20 μL rxns
$449
$314
CGCGATATCGATCGATCGCGATATCGATCGATCGCGATATCGATCGATCGCGA
F-455L
DyNAmo Flash Probe qPCR Kit
500 x 20 μL rxns
$449
$314
CGATCGATCGCGATATCGATCGATCGCGATATCGTCGATCGATCGCGATATCG
K1641
50
rxns
$357
$250
Maxima First Strand cDNA Synthesis Kit for RT-qPCR
G
Acomponents)
TCGCGATATCGATCGATCGCGATATCGATCGATCGCGATATCGATCGATCG
(pre-mixed
kit
K1642
200 rxns
$1122
$785
TATCGATCGATCGCGATATCGATCGATCGCGATATCGATCGATCGCGATATCG
Maxima Universal First Strand cDNA Synthesis Kit
K1661
G A TinCseparate
G C G A tubes)
T A T C G A T C G A T C G C50Grxns
A T A T C G A T C $357
G A T C G C$250
GATGCGATATCGATC
(kit components
C G31,
A T2012.
CGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGGAT
Offers are valid until March
TCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCGATCG
Cat. No
Description
Order Your Free Thermo Scientific PCR and qPCR Product Guide
Benefits
• State-of-the-art products from the Thermo Scientific
ABgene, Finnzymes and Fermentas product lines
• Contains a complete workflow of PCR and qPCR products
including reagents, instruments and plastic consumables.
• Features product selection tables to assist in finding the
best solution for your application
Order a free copy at
www.thermoscientific.com/pcr
For further information visit
www.thermoscientific.com/mpcda
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