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Part of Thermo Fisher Scientific
GeneJET™ PCR Purification Kit
Fast and efficient purification of DNA from PCR mixes
Features
• Highly efficient – 90-100% recoveries in the range of
100 bp – 10 kb.
• Fast – procedure takes only 5 min.
• Convenient – spin columns are capped and assembled with
collection tubes.
Applications
• Fast isolation of high purity DNA suitable for all conventional
molecular biology procedures, including:
– FastDigest® or conventional restriction digestion,
– automated fluorescent sequencing,
– PCR,
– in vitro transcription,
– in situ hybridization,
– labeling,
– cloning.
Description
The GeneJET™ PCR Purification Kit is designed for rapid and
efficient purification of DNA from PCR and other enzymatic reaction
mixtures.
The kit utilizes a proprietary silica-based membrane technology
in the form of a convenient spin column, eliminating the need for
tedious resin manipulations or toxic phenol-chloroform extractions.
The GeneJET™ PCR Purification Kit effectively removes primers,
dNTPs, unincorporated labeled nucleotides, enzymes and salts
from PCR and other reaction mixtures. The kit can be used for purification of DNA fragments from 25 bp to 20 kb with recovery rates
up to 100%. Each GeneJET™ purification column has a total binding
capacity of up to 25 µg of DNA and the entire procedure takes just
5 min. The purified DNA can be used in common downstream applications such as sequencing, restriction digestion, labeling, ligation,
cloning, in vitro transcription, blotting or in situ hybridization.
Recovery rate, %
100
60
40
20
0
Direct sequencing of purified DNA
Purified PCR fragment was sequenced using ABI PRISM 377 DNA Analyzer. More
than 650 nucleotides can be read.
Product
GeneJET™ PCR Purification Kit
Cat. #
Size
K0701
50 preps
K0702
250 preps
Fermentas
vendor A
vendor B
80
100 bp
500 bp
1 kb
Size
5.5 kb
Superior purification of DNA fragments from PCR mixture
10 kb
Part of Thermo Fisher Scientific
GeneJET™ Gel Extraction Kit
Highly efficient extraction of DNA from agarose gels
Features
• Highly efficient – up to 95% recoveries in the range
of 100 bp – 10 kb.
• Fast – procedure takes only 15 min.
• Convenient – spin columns are capped and assembled with
collection tubes.
Applications
• Fast extraction of high purity DNA suitable for all conventional
molecular biology procedures, including:
– FastDigest® or conventional restriction digestion,
– automated fluorescent sequencing,
– PCR,
– in situ hybridization,
– labeling,
– cloning.
Fermentas
vendor A
vendor B
100
Recovery rate, %
Description
The GeneJET™ Gel Extraction Kit is designed for rapid and efficient
purification of DNA fragments from standard or low-melting point
agarose gels run in either TAE or TBE buffer.
The kit utilizes a proprietary silica-based membrane technology in
the form of a convenient spin column. The kit can be used to purify
DNA fragments from 25 bp to 20 kb in size with recovery rates up
to 95%. Each GeneJET™ purification column has a binding capacity
of up to 25 µg of DNA and can process up to 1 g of agarose gel.
The entire procedure takes just 15 min and the isolated DNA is
ready to use in all common downstream applications including ligation, restriction digestion, PCR, sequencing and labeling.
80
60
40
Spin column is capped and
assembled with collection tube
20
0
100 bp
500 bp
1 kb
Size
5.5 kb
10 kb
Superior extraction of DNA from agarose gels
Product
GeneJET™ Gel Extraction Kit
Cat. #
Size
K0691
50 preps
K0692
250 preps
PureExtreme® is registered trademark of Fermentas. GeneJET™ is trademark of Fermentas.