Teacher Guide and Answers, continued Lab 5 • Classic How do we measure biodiversity? Data and Observations Sample graph Annual Average Grams of Biomass Objectives 120 160 Process Skills grams biomass per 0.3 m2 100 120 80 100 60 80 60 40 40 20 20 0 0 graph, model, infer, predict, draw conclusions 1982 1983 1984 1985 1986 1987 1988 1989 1990 1991 1992 Year Time Allotment Community 1 45 minutes Community 3 Community 2 Community 4 Materials pen graph paper ruler cm • Analyze data from four test sites. • Infer trends in biodiversity. • Predict what environmental factors impact biodiversity. 140 colored pencils calculator Alternative Materials • You might want students to graph the data using a computer and graphing program. Preparation Teaching the Lab • Make sure students choose appropriate scales for their graph. • Explain that each of the farms has been abandoned and then left undisturbed. Secondary succession is slowly returning each site to its natural habitat. • Assist students in their understanding of how the original data was collected. The average of grams of biomass per site was determined by collecting, drying, and measuring the mass of all the plant material that could be clipped from a 0.3-m2 area. The study was done over the course of 11 years at the Cedar Creek Natural History Area just north of the Minneapolis-St. Paul metropolitan area. The natural disaster of a drought during 1987–1988 provided an unusual opportunity to measure biomass effects and recovery in the area. 168 TEACHER GUIDE AND ANSWERS Analyze and Conclude 1. Organisms will likely include field grasses (forbes or weeds), small shrubs, rodents, insects, birds, and larger animals such as coyotes. 2. Community 1 is most diverse. The data indicate that Community 1 consistently had greater biomass, and even after the drought, it was able to show the most biomass. 3. The precipitation data indicate a period of drought from approximately 1987–1989. The biodiversity in all communities decreased during this time. 4. Community 2 had the greatest change in biomass. This is probably because there is less diversity in the community. The community with the least change is Community 1. Because of its greater diversity, its overall biomass remained at more consistent levels. 5. Community 1 recovered most quickly. Community 2 recovered most slowly. 6. Inability to analyze the data presented could be a source of error. 7. It appears that the greater the diversity and the more biomass in a community, the more stable it is. Inquiry Extensions 1. Students could cite events such as fire, flooding, or disease invasion as factors. Their predictions should show the greatest impact on and slowest recovery in Community 2. Communities with the Laboratory Manual Copyright © Glencoe/McGraw-Hill, a division of The McGraw-Hill Companies, Inc. • Make sure you have enough graph paper for each student • Review graphing techniques with students if necessary. Annual Precipitation Teacher Guide and Answers, continued most diverse biomass would rebound faster because the soil has more nutrients to support new growth. 2. Students could design an experiment in which an affected area is examined over a period of time. Real-estate development would adversely affect biodiversity. In addition to loss of biomass, students could mention habitat destruction, changes in water runoff patterns, or wind erosion. Because three of the communities were once farms, students might try to analyze the impact of fertilizers and pesticides. Human impact in the form of conservation efforts could result in more rapid naturalization through selected species re-introduction, protection for water, or removal of litter and pollutants. Lab 6 • Design Your Own How much vitamin C are you getting? Copyright © Glencoe/McGraw-Hill, a division of The McGraw-Hill Companies, Inc. Objectives • Make predictions about the amounts of vitamin C in a variety of drinks. • Design an experiment to compare the amounts of vitamin C in these beverages. • Measure how different substances react in the presence of iodine. • Draw conclusions about the nutritional value of the beverages. Process Skills measure, make predictions, design an experiment, draw conclusions, use titration as a testing method Materials 50-mL beakers (6) plastic droppers starch solution tincture of iodine vitamin-C solution orange juice juices and sports drinks with added vitamin C (4) (Note: Because the students will be looking for a blue-purple color change, do not provide drinks that are dark in color.) envelope containing copies of nutritional information labels keyed to each drink, to be distributed after the students complete the procedure Laboratory Manual Alternative Materials • Any type of juice or sports drink that makes a claim about vitamin-C content can be used. Possible Hypothesis Orange juice has the most vitamin C; therefore, a sample of orange juice will require the most drops of iodine to observe a reaction with the starch. Possible Procedure 1. Choose five beverages to test. Predict which one has the highest level of vitamin C and which has the lowest. Rank each sample according to the prediction. 2. Create a data table to record this information. 3. Set out and label six 50-mL beakers. In the first beaker, place 25 mL of vitamin-C solution. Add 25 mL of each beverage to its own beaker. 4. Add two drops of starch solution to each beaker. 5. Beginning with the vitamin-C solution, add one drop of iodine to the beaker, and gently swirl. Observe any change. Continue to add the iodine, one drop at a time, until the liquid turns a dark blue color. Record the number of iodine drops needed to form this color. 6. Repeat step 5 for the beverages in the remaining five beakers. Preparation • Prepare the starch solution by directing a stream of spray starch into 75–100 mL of water in a beaker while stirring rapidly. A mixture at the correct strength will have a pale blue tinge. • Tincture of iodine, which is used as a skin antiseptic, works well. Use a brand that comes with a dropper. • Prepare a 1-percent ascorbic-acid solution. [See Preparation of Solutions, pp. T13–T15.] • Small containers of beverages will provide enough samples for a class. • Choose a variety of juices and sports or energy drinks that students might be familiar with or drink on a daily basis. This will make the experiment more interesting for them. TEACHER GUIDE AND ANSWERS 169