MATERIAL SAFETY DATA SHEET
Section 1.
Product Name:
Product and Company Information
ProteaPrep Non-ionic Cell Lysis Kit, Mass Spec Grade
Catalog Numbers: SP-817-10mL
Harmonized Code: 3402195000
Revision Date:
10/03/11
Section 2.


Company:
Protea Biosciences, Inc.
955 Hartman Run Road
Morgantown, WV 26507 USA
P: 304.292.2226 ● F: 304.292.7101
www.proteabio.com
Composition / Information on Components
Chemical Characterization
Description: Mixture of the substances below with non-hazardous additions.
CAS #
1132-61-2
N/A
7647-14-5
15471-17-7
77-86-1
7732-18-5
Section 3.
Listing of dangerous and non-hazardous components:
Chemical Name
EC #
4-Morpholinopropanesulphonic acid (MOPS)
214-478-5
Non-ionic Acid Labile Surfactant I
N/A
(trade secret)
Sodium Chloride
231-598-3
3-(1-Pyridinio)-1-Propanesulfonate
239-491-3
Tris (hydroxymethyl) aminomethane base
214-478-5
Water
231-791-2
Percent
≤1%
1-2%
≤1%
≤1%
≤1%
95-98%
Hazards Identification
EMERGENCY OVERVIEW
Appearance: Colorless Liquid
Caution! Irritating to eyes, respiratory system, and skin
Ingestion: Harmful by ingestion
Inhalation: Harmful by inhalation
Information pertaining to particular dangers for man and environment:
Limited evidence of a carcinogenic effect.
NFPA ratings (scale 0 to 4)
Health = 2
Fire = 1
Reactivity = 0
Section 4.
First Aid Measures
General Information:
Eyes: Flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids. Get
medical aid.
Skin: Get medical aid if irritation develops or persists. Wash clothing before reuse. Flush skin with plenty of soap and
water.
Ingestion: If victim is conscious and alert, give 2-4 cupfuls of milk or water. Treat symptomatically and supportively.
Get medical aid immediately.
Inhalation: Remove from exposure and move to fresh air immediately. If not breathing, give artificial respiration. If
breathing is difficult, give oxygen. Get medical aid if cough or other symptoms appear.
Notes to Physician: Treat symptomatically and supportively.
Section 5.
Fire Fighting Measures
General Information: As in any fire, wear a self-contained breathing apparatus in pressure-demand, MSHA/NIOSH
(approved or equivalent), and full protective gear. Dusts at sufficient concentrations can form explosive mixtures with
air. Combustion generates toxic fumes.
Extinguishing Media: Use water spray, dry chemical, carbon dioxide, or chemical foam.
Flash Point: Not available.
Section 6.
Accidental Release Measures
General Information: Use proper personal protective equipment as indicated in Section 8.
Spills/Leaks: A vacuum or sweep up material and place into a suitable disposal container. Clean up spills
immediately, observing precautions in the Protective Equipment section. Do not allow to enter sewers, surface, or
ground water. Absorb with liquid-binding material (sand, diatomite, acid binders, universal binders, sawdust).
Section 7.
Handling and Storage
Handling: No special measures required. Avoid contact with eyes, skin, and clothing. Keep container tightly closed.
Storage: Store at 4°C.
Section 8.
Exposure Control / Personal Protection
Engineering Controls: Facilities storing or utilizing this material should be equipped with an eyewash facility and a
safety shower. Use adequate ventilation to keep airborne concentrations low.
Exposure Limits:
4-Morpholinopropanesulphonic acid, 1132-61-2
PEL
None listed
Non-ionic Acid Labile Surfactant I, N/A
PEL
None listed
Sodium Chloride, 7647-14-5
PEL
None listed
3-(1-Pyridinio)-1-Propanesulfonate, 15471-17-7
PEL
None listed
Tris, 77-86-1
PEL
None listed
Water, 7732-18-5
PEL
None listed
OSHA Vacated PELs:
4-Morpholinopropanesulphonic acid: No OSHA Vacated PELs are listed for this chemical.
Non-ionic Acid Labile Surfactant I: No OSHA Vacated PELs are listed for this chemical.
Sodium Chloride: No OSHA Vacated PELs are listed for this chemical.
3-(1-Pyridinio)-1-Propanesulfonate: No OSHA Vacated PELs are listed for this chemical.
Tris: No OSHA Vacated PELs are listed for this chemical.
Water: No OSHA Vacated PELs are listed for this chemical.
Personal Protective Equipment
Eyes: Wear appropriate protective eyeglasses or chemical safety goggles as described by OSHA's eye and face
protection regulations in 29 CFR 1910.133 or European Standard EN166.
Skin: Wear appropriate gloves to prevent skin exposure.
Clothing: Wear appropriate protective clothing to prevent skin exposure.
Respirators: Follow the OSHA respirator regulations found in 29 CFR 1910.134 or European Standard EN 149. Use
a NIOSH/MSHA or European Standard EN 149 approved respirator if exposure limits are exceeded or if irritation or
other symptoms are experienced.
Section 9.
Physical and Chemical Properties
Physical State: film
Appearance: white
Odor: odorless
pH: Not available.
Vapor Pressure: Not available.
Vapor Density: Not available.
Evaporation Rate: Not available.
Section 10.
Boiling Point: Not available.
Melting Point: Not available.
Decomposition Temperature: Not available.
Solubility: Fully miscible in water.
Specific Gravity/Density: ~1.0.
Molecular Formula: Not available.
Molecular Weight: Not available.
Stability and Reactivity
Chemical Stability: Stable under normal temperatures and pressures.
Thermal decomposition / conditions to be avoided: Incompatible materials, dust generation, excess heat.
Dangerous reactions: Strong oxidizing agents.
Hazardous Decomposition Products: Will not occur.
Hazardous Polymerization: Will not occur.
Section 11.
Toxicological Information
ProteaPrep Non-ionic Cell Lysis Kit Reagent
RTECS#:
CAS# 1132-61-2
CAS# N/A
CAS# 7647-14-5
CAS# 15471-17-7
CAS# 77-86-1
CAS# 7732-18-5
Carcinogenicity:
Not listed by ACGIH, IARC, NTP, or CA Prop 65.
Epidemiology: No data available.
Teratogenicity: No data available.
Reproductive Effects: No data available.
LD50/LC50:
CAS# 1132-61-2 LD50 Oral-rat >2000mg/kg
CAS# 7647-14-5 LD50 Oral-rat >3000mg/kg
LC50 Inhalation-rat-1hr >42000mg/m3
LD50 Dermal-rabbit >10000mg/kg
CAS# 77-86-1 LD50 Oral-rat >5900 mg/kg
Section 12.
Mutagenicity: Laboratory experiments have shown
mutagenic effects.
Neurotoxicity: No data available.
Ecological Information
Ecotoxicity: No data available. No information available.
Environmental: Water hazard class 1: slightly hazardous for water. Do not allow undiluted product or large
quantities of it to reach ground water, water course, or sewage system.
Physical: No information available.
Section 13.
Disposal Considerations
Chemical waste generators must determine whether a discarded chemical is classified as a hazardous waste. US
EPA guidelines for the classification determination are listed in 40 CFR Parts 261.3. Additionally, waste generators
must consult state and local hazardous waste regulations to ensure complete and accurate classification.
RCRA P-Series: None listed
RCRA U-Series: None listed
Section 14.
Transport Information
DOT Regulations:
Hazard Class: non-hazardous – This substance is considered to be non-hazardous for transport.
Land transport ADR/RID (cross border):
ADR/RID Class: Maritime transport IMDG:
IMDG Class: Marine Pollutant: No
Air transport ICAO-TI and IATA-DGR:
ICAO/IATA Class: non-hazardous -- This substance is considered to be non-hazardous for air transport.
Transport / Additional information: Not dangerous according to the above specifications.
Section 15.
Regulatory Information
US Federal
TSCA
CAS: 1132-61-2 is listed on the TSCA Inventory
CAS: 7647-14-5 is listed on the TSCA Inventory
CAS: 15471-17-7 is listed on the TSCA Inventory
CAS: 77-86-1 is listed on the TSCA Inventory
CAS: 7732-18-5 is listed on the TSCA Inventory
SARA Reportable Quantities (RQ)
None of the components are on this list.
CERCLA/SARA Section 313
None of the components are on this list.
OSHA – Highly Hazardous
None of the components are on this list
US State
State Right to Know
CAS# 1132-61-2 is present on state lists from CA,
PA, MN, MA, FL, or NJ.
CAS# N/A is not present on state lists from CA, PA,
MN, MA, FL, or NJ.
Section 16.
CAS# 7647-14-5 is present on state lists from CA,
PA, MN, MA, FL, or NJ.
CAS# 15471-17-7 is present on state lists from CA,
PA, MN, MA, FL, or NJ.
CAS# 77-86-1 is present on state lists from CA, PA,
MN, MA, FL, or NJ.
CAS# 7732-18-5 is not present on state lists from CA,
PA, MN, MA, FL, or NJ.
European/International Regulations
Canada Ingredient Disclosure List
CAS# 1132-61-2: is not listed on Canada’s Ingredient
Disclosure List.
CAS# N/A is not listed on Canada’s Ingredient
Disclosure List.
CAS# 7647-14-5 is not listed on Canada’s Ingredient
Disclosure List.
CAS# 15471-17-7 is not listed on Canada’s Ingredient
Disclosure List.
CAS# 77-86-1 is not listed on Canada’s Ingredient
Disclosure List.
CAS# 7732-18-5 is not listed on Canada’s Ingredient
Disclosure List.
Other Information
MSDS Creation Date: 10/03/2011
Revision #1 Date: 02/20/2012
The information above is believed to be accurate and represents the best information currently available to us.
However, we make no warranty of merchantability or any other warranty, express or implied, with respect to such
information, and we assume no liability resulting from its use. Users should make their own investigations to
determine the suitability of the information for their particular purposes. In no event shall Protea Biosciences be liable
for any claims, losses, or damages of any third party or for lost profits or any special, indirect, incidental,
consequential or exemplary damages, howsoever arising, even if Protea Biosciences has been advised of the
possibility of such damages.