MATERIAL SAFETY DATA SHEET
Section 1.
Product Name:
Product and Company Information
GPR Electroelution Buffer I, Tris-Glycine
Catalog Numbers: GPR-020-25mL
UN number:
UN1230
Harmonized Code: 2905110000
Revision Date:
10/15/09
Section 2.
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Company:
Protea Biosciences, Inc.
955 Hartman Run Road
Morgantown, WV 26507 USA
P: 304.292.2226 ● F: 304.292.7101
www.proteabio.com
Composition / Information on Components
Chemical Characterization
Description: Mixture of the substances below with non-hazardous additions.
Listing of dangerous and non-hazardous components:
CAS #
Chemical Name
7732-18-5
Water
56-40-6
Glycine
EC #
231-791-2
200-272-2
Percent
80-85%
1.0-5.0%
77-86-1
67-56-1
Tris
Methanol
201-064-4
200-659-6
0.1-1.0%
10%
N/A
Anionic Acid Labile Surfactant (trade secret)
N/A
0.5%
Section 3.
Hazards Identification
EMERGENCY OVERVIEW
Appearance: whitish liquid
Caution! May cause eye and skin irritation.
Ingestion: May cause gastrointestinal irritation with nausea, vomiting and diarrhea. May cause systemic toxicity with
acidosis. May cause central nervous system depression, characterized by excitement, followed by headache,
dizziness, drowsiness, and nausea.
Inhalation: May cause CNS depression with nausea, headache, vomiting, dizziness and incoordination.
Chronic: Prolonged or repeated skin contact may cause dermatitis. Chronic exposure may cause effects similar to
those of acute exposure.
Information pertaining to particular dangers for man and environment: Not applicable.
Classification system
The classification was made according to the latest editions of the EU-lists.
NFPA ratings (scale 0 to 4)
Health = 1
Fire = 1
Reactivity = 0
Section 4.
First Aid Measures
General Information: No special measures required.
Eyes: Flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids. Get
medical aid.
Skin: Get medical aid if irritation develops or persists. Wash clothing before reuse. Flush skin with plenty of soap and
water.
Ingestion: If victim is conscious and alert, give 2-4 cupfuls of milk or water. Treat symptomatically and supportively.
Get medical aid immediately.
Inhalation: Remove from exposure and move to fresh air immediately. If not breathing, give artificial respiration. If
breathing is difficult, give oxygen. Get medical aid if cough or other symptoms appear.
Notes to Physician: Treat symptomatically and supportively.
Section 5.
Fire Fighting Measures
General Information: As in any fire, wear a self-contained breathing apparatus in pressure-demand, MSHA/NIOSH
(approved or equivalent), and full protective gear. Dusts at sufficient concentrations can form explosive mixtures with
air. Combustion generates toxic fumes.
Extinguishing Media: Use water spray, dry chemical, carbon dioxide, or chemical foam.
Flash Point: Not available.
Section 6.
Accidental Release Measures
General Information: Use proper personal protective equipment as indicated in Section 8.
Spills/Leaks: A vacuum or sweep up material and place into a suitable disposal container. Clean up spills
immediately, observing precautions in the Protective Equipment section. Do not allow to enter sewers, surface, or
ground water. Absorb with liquid-binding material (sand, diatomite, acid binders, universal binders, sawdust).
Section 7.
Handling and Storage
Handling: No special measures required. Avoid contact with eyes, skin, and clothing. Keep container tightly closed.
Storage: Store at 4 to -20°C.
Section 8.
Exposure Control / Personal Protection
Engineering Controls: Facilities storing or utilizing this material should be equipped with an eyewash facility and a
safety shower. Use adequate ventilation to keep airborne concentrations low.
Exposure Limits:
Water, 7732-18-5
PEL
Glycine, 56-40-6
PEL
Tris(hydroxymethyl)aminomethane, 77-86-1
PEL
Methanol, 67-56-1
PEL
Anionic Acid Labile Surfactant, N/A
PEL
None listed
None listed
None listed
200 ppm TWA; 260 mg/m3 TWA
None Listed
OSHA Vacated PELs:
Water: No OSHA Vacated PELs are listed for this chemical.
Glycine: No OSHA Vacated PELs are listed for this chemical.
Tris(hydroxymethyl)aminomethane : No OSHA Vacated PELs are listed for this chemical.
Methanol: 200 ppm TWA; 260 mg/m3 TWA
Anionic Acid Labile Surfactant: No OSHA Vacated PELs are listed for this chemical.
Personal Protective Equipment
Eyes: Wear appropriate protective eyeglasses or chemical safety goggles as described by OSHA's eye and face
protection regulations in 29 CFR 1910.133 or European Standard EN166.
Skin: Wear appropriate gloves to prevent skin exposure.
Clothing: Wear appropriate protective clothing to prevent skin exposure.
Respirators: Follow the OSHA respirator regulations found in 29 CFR 1910.134 or European Standard EN 149. Use
a NIOSH/MSHA or European Standard EN 149 approved respirator if exposure limits are exceeded or if irritation or
other symptoms are experienced.
Section 9.
Physical and Chemical Properties
Physical State: liquid
Appearance: Whitish
Odor: odorless
pH: ~8.0-9.0
Vapor Pressure: Not available.
Vapor Density: Not available.
Evaporation Rate: Not available.
Section 10.
Boiling Point: Not available.
Melting Point: Not available.
Decomposition Temperature: Not available.
Solubility: Fully miscible in water.
Specific Gravity/Density: Not available.
Molecular Formula: Not available.
Molecular Weight: Not available.
Stability and Reactivity
Chemical Stability: Stable under normal temperatures and pressures.
Thermal decomposition / conditions to be avoided: Incompatible materials, dust generation, excess heat.
Dangerous reactions: Strong oxidizing agents.
Hazardous Decomposition Products: Carbon monoxide, carbon dioxide, formaldehyde.
Hazardous Polymerization: Will not occur.
Section 11.
Toxicological Information
GPR Electroelution Buffer GPR-020-10mL
RTECS#:
CAS# 7732-18-5
CAS# 56-40-6
CAS# 77-86-1
CAS# 67-56-1
Anionic Acid Labile Surfactant CAS# N/A
Carcinogenicity: No ingredients listed.
LD50/LC50:
Not available.
Mutagenicity: No ingredients listed.
Epidemiology: No ingredients listed.
Teratogenicity: No ingredients listed.
Reproductive Effects: No ingredients listed.
Neurotoxicity: No ingredients listed.
Section 12.
Ecological Information
Ecotoxicity: No data available. No information available.
Environmental: Water hazard class 1: slightly hazardous for water. Do not allow undiluted product or large
quantities of it to reach ground water, water course, or sewage system.
Physical: No information available.
Section 13.
Disposal Considerations
Chemical waste generators must determine whether a discarded chemical is classified as a hazardous waste. US
EPA guidelines for the classification determination are listed in 40 CFR Parts 261.3. Additionally, waste generators
must consult state and local hazardous waste regulations to ensure complete and accurate classification.
RCRA P-Series: None listed.
RCRA U-Series: CAS# 67-56-1: waste number U154 (Ignitable waste).
Section 14.
Transport Information
DOT Regulations:
Hazard Class: non-hazardous – This substance is considered to be non-hazardous for transport.
Land transport ADR/RID (cross border):
ADR/RID Class: Maritime transport IMDG:
IMDG Class: Marine Pollutant: No
Air transport ICAO-TI and IATA-DGR:
ICAO/IATA Class: non-hazardous -- This substance is considered to be non-hazardous for air transport.
Transport / Additional information: Not dangerous according to the above specifications.
Section 15.
Regulatory Information
US Federal
TSCA
CAS: 7732-18-5 is listed on the TSCA Inventory
CAS: 56-40-6 is listed on the TSCA Inventory
CAS: 77-86-1 is listed on the TSCA Inventory
CAS: 67-56-1 is listed on the TSCA Inventory
SARA Reportable Quantities (RQ)
None of the components are on this list
CERCLA/SARA Section 313
None of the components are on this list
OSHA – Highly Hazardous
None of the components are on this list
US State
State Right to Know
CAS# 7732-18-5 is not present on state lists from CA, PA, MN, MA, FL, or NJ.
CAS# 56-40-6 is not present on state lists from CA, PA, MN, MA, FL, or NJ.
CAS# 77-86-1 is not present on state lists from CA, PA, MN, MA, FL, or NJ.
CAS# 67-56-1 is present on state lists from CA, PA, MN, MA, FL, or NJ.
Anionic Acid Labile Surfactant is not present on state lists from CA, PA, MN, MA, FL, or NJ.
European/International Regulations
Canada Ingredient Disclosure List
CAS# 7732-18-5 is not listed on Canada’s Ingredient Disclosure List.
CAS# 56-40-6 is not listed on Canada’s Ingredient Disclosure List.
CAS# 77-86-1 is not listed on Canada’s Ingredient Disclosure List.
CAS# 67-56-1 is listed on Canada’s Ingredient Disclosure List.
Anionic Acid Labile Surfactant is not listed on Canada’s Ingredient Disclosure List.
Section 16.
Other Information
MSDS Creation Date: 07/08/2008
Revision #1 Date: 04/09/2009
The information above is believed to be accurate and represents the best information currently available to us.
However, we make no warranty of merchantability or any other warranty, express or implied, with respect to such
information, and we assume no liability resulting from its use. Users should make their own investigations to
determine the suitability of the information for their particular purposes. In no event shall Protea Biosciences be liable
for any claims, losses, or damages of any third party or for lost profits or any special, indirect, incidental,
consequential or exemplary damages, howsoever arising, even if Protea Biosciences has been advised of the
possibility of such damages.