BACTERIAL FEATURES ON PREPARED SLIDES
http://biology.clc.uc.edu/fankhauser/Labs/Microbiology/Prepared_Slides/Bacterial_Anatomy.htm
The box of slides you ha ve previously signed out co ntain specimens which disp lay significant features o f bacteria.
As you draw these bacteria in your notebook, be sure to note the significance of each specimen, the stain used, and
how the specimen may d iffer from other b acteria. (See the Index of S lides at the beginning of this manual.)
FEATURES OF BACTERIAL ANATOMY, SELECTED SPECIES
Slide
in
box
3
catalog
number
species
90 W 2021 Bacillus anthra cis
features
commen ts
strepto bacilli,
central spores
Gram stain, First etiological agent (of anthrax),
identified by Koch. Gram positive.
A special stain (para rosan iline) has b een used to
build up the flagella so that they can be seen on
this bacillus. Causes UTI.
5
Ba 156
Proteu s vulgaris
peritrichous flagella
6
Ba 016a
Unspecified
species
Capsules do not take up Negatively stained (with congo red or eosin? ) to
acid stain, the
show the presence of the mucopolysaccharide
backgro und do es.
"slime" capsule.
4
7
90 W 2054 Mycobacterium
tubercu losis
Ba 133
Neisse ria
gonorrhoeae
Clusters of red staining Acid Fast Stain This acid fast stained slide
tiny filamentous
shows the red pleomorphic shapes of the
bacteria among blue
etiological agent of tube rculosis.
stained sputum.
Gram ! diplo cocci in
leukocyte (PMN)
cytoplasm
Gram stained smear of a purulent urethral
discharge. Large numbers of
polym orphonucleoc ytes (PMN) are pre sent with
a few squamous cells. In the cytoplasm of the
PMNs can be seen phagocytosed Gm !
diplococci which are diagnostic of gonorrheal
infection.
SPO RE FO RM ING BA CT ER IA
17
Ba 60
Clostridium tetani terminal “lollipop”
spores
16
Ba 45
Clostridium
botulinum
Gra m Stain. Etiolo gical agent of tetanus. Cells
with terminal spo res loo k like “lollyp ops.”
terminal oblong spores Gra m Stain. These cells bearing spo res are only
(‘slipper” shaped)
slightly enlarged at the spore containing end in
this etiological agent of botulism.
15
90 W 0533 Bacillus subtilis
3
90 W 2021 Bacillus anthra cis strepto bacilli,
(a rep eat)
central spores
aerobic endospore
forming
Ubiquitous soil bacterium, used in commercial
production of enzymes
First etiological agent (of anthrax), identified by
Ko ch. Ce ntral spo res. W e view this slide again
because it is one of the two spore forming
genera we are studying, Bacillus and
Clostridium.