Types of Microorganisms Learning objective • Differentiate among the major characteristics of each group of microorganisms. BACTERIA (figure 1) Bacteria (singular: bacterium) are single celled (unicellular) organisms. Because their genetic material is not enclosed in a special nuclear membrane, they are called prokaryotes. Bacteria are enclosed in cell walls that are largely composed of a carbohydrate and protein complex called peptidoglycan. They reproduce by dividing into two equal cells; binary fission. Most bacteria use organic chemicals around them. Some manufacture their own food by photosynthesis, and some derive nutrition from inorganic substances. Many can “swim” by using appendages called flagella. ARCHAEA Archaea consist of prokaryotic cells, but if they have cell walls, they lack peptidoglycans. They are divided into 3 major groups: methanogens produce methane, extreme halophiles live in extremely salty environments and extreme thermophiles which live in hot springs. FUNGI Fungi are eukaryotes, which are organisms which have a distinct nucleus containing cell’s genetic material (DNA) surrounded by a special envelope called the nuclear membrane. Fungi may be unicellular or multicellular. True fungi have cell walls composed primarily of chitin. The unicellular forms are larger than bacteria. The most typical fungi are molds which form visible masses called mycelia which are composed of long filaments (hyphae) that branch and intertwine. They reproduce sexually or asexually. PROTOZOA Are unicellular, eukaryotic microbes. Amoebas move by extensions of their cytoplasm known as pseudopods. Others move by flagella, or shorter appendages called cilia. They live either as free entities or as parasites (organisms that derive their nutrition from living hosts). They reproduce sexually or a sexually. ALGAE Are photosynthetic eukaryotes with both sexual and asexual reproduction. The cell walls are composed of a carbohydrate called cellulose. Algae are abundant in fresh and salt water and in soil. They need light, water and carbon dioxide for food production and growth. They then produce oxygen and carbohydrates. VIRUSES They are very different form the other microbial groups. They are so small that they can be seen only under an electron microscope. They are acellular (not cellular), structurally very simple with a core that contains only one type of nucleic acid, either DNA or RNA. The core is surrounded by a protein coat and sometimes encased by an envelope. Viruses can reproduce only by using the cellular machinery of other organisms. Thus, on one hand, they are considered to be living when they multiply within host cells they infect. On the other hand, they are not considered to be living because outside of living hosts they are inert. MULTICELLULAR ANIMAL PARASITES The 2 major groups of parasitic worms, the flatworms and the round worms, are collectively called helminths. A bacillus, one of the several shapes of bacteria Mucor, a type of fungus HIV virus budding from a lymphocyte Amoeba Fig 1. Types of microorganisms A pond alga Prokaryotic and Eukaryotic Cells Learning objective • Compare and contrast the overall cell structure of prokaryotes and eukaryotes All living cells can be classified into 2 groups, prokaryotes and eukaryotes, based on certain structural and functional characteristics (Table 1). Plants and animals, in addition to some cellular microbes (fungi, protozoa and algae) are eukaryotes. Bacteria and Archaea are prokaryotes. Table1. Principle differences between Prokaryotic and Eukaryotic cells Characteristic Prokaryotic Eukaryotic Size of cell Nucleus Typically 0.2-2.0 µm in diameter No nuclear membrane or nucleoli Membrane-enclosed organelles Absent Flagella Consist of 2 protein building blocks Present as a capsule or slime layer Usually present; chemically complex with peptidoglycan No carbohydrates and generally lacks sterols No cytoskeleton Smaller size (70S) Usually single circular chromosome; typically lacks histones Binary fission None; transfer of DNA fragments only Glycocalyx Cell wall Plasma membrane Cytoplasm Ribosomes Chromosome (DNA) Cell division Sexual recombination Typically 10-100 µm in diameter True nucleus, consisting of nuclear membrane and nucleoli Present (lysosomes, Golgi complex, endoplasmic reticulum, mitochondria, chloroplasts) Complex; consist of multiple microtubules Present in some cells that lack a cell wall When present, chemically simple Sterols and carbohydrates that serve as receptors Cytoskeleton Larger size (80 S) Multiple linear chromosomes with histones Involves mitosis Involves meiosis