赣 南 医 学 院 教 案
教研室：Department of biochemistry
课程名称
Biochemistry
教师姓名： Liu Lihua
授课专业
Clinical medicine major
授课内容
Experiment 2 The effects of temperature, pH,
activator and inhibitor on enzyme activity
教学目的
1．Master the basic principles of temperature, pH, activator and inhibitor to the
effects of enzyme activity.
2．Master the enzymatic reaction catalyzed by saliva amylase.
3．Know the usage and operation of some instruments.
教学重点
the basic principles of temperature, pH, activator and inhibitor to the effects of
enzyme activity.
教学难点
the basic principles of temperature, pH, activator and inhibitor to the effects of
enzyme activity.
教具和媒
体使用
computer, projector, mike, chalk, ferule, multimedia courseware, instruments.
教学方法
Problem based on learning methods
授课学时
4
1. Effect of temperature on enzyme activity
教学过程
2. Effect of pH on enzyme activity
3. Effect of activator and inhibitor on enzyme activity
All chemical reactions are affected by temperature. The effects of it on the
enzyme activity are bidirectional. Among certain range, the velocity of
enzyme-catalyzed reactions will increases as temperature rise. When the velocity
gets to the maximum, the temperature is optimum temperature. Whereas the
velocity will decrease as temperature rise further more. For human, the optimum
temperature of enzymes is usually close to the body temperature: 35℃－40℃. If
the temperature rises above the optimal temperature, activities of many enzymes
will decline sharply, mainly owing to their heat denaturation.
课后总结
pH is an important environmental factor for enzyme-catalyzed reactions.
Most enzymes are active only in a narrow pH range. When the velocity gets to
the maximum, the pH is optimum pH, whereas the velocity will decrease far
from optimum pH. For human saliva amylase, optimum pH is near to 7.0.
Activators and inhibitors can also affect enzyme activity. The substances
that inhibit or accelerate the enzymatic reaction are called inhibitors or activators.
For example, Cl － (chlorine ion) can increase the activity of enzyme, but
Cu2+(copper ion) can depress the activity of enzyme.