UNIVERSITY OF WESTMINSTER
School of Biosciences
Control of Substances Hazardous to Health (COSHH)
***** APPENDIX 4 - CHEMICAL SAFETY *****
Sheet........... of............
Title of Experiment
Room/Laboratory
Site
Sandwich ELISA Development with OPD
5.08
NCS
1.
Brief description of work
Sandwich ELISA developed with OPD
Note that a COSHH assessment for class activity may not be of a sufficiently high level to cover technician preparation; double assessment usually
required. Note also that it is RISK that is to be assessed, not HAZARD.
2.
LIST OF SUBSTANCES USED OR CREATED (continue on a separate sheet if necessary)
NAME OF SUBSTANCE
(CODE)
PHYSICAL
DESCRIPTION
Sodium bicarbonate
Solid
MAX.
QUANTITY
EXPOSED
TO
G
DATA AVAILABLE
(YES/NO)
Sodium hydrogen carbonate
Solid
G
Y
Sodium chloride (salt)
Solid
G
Y
Potassium chloride
Solid
G
Y
di-sodium hydrogen phosphate
Solid
G
y
Potassium dihydrogen phosphate
Solid
G
Y
Sulphuric acid
Liquid
Ml
Y
Citric acid
Solid
Liquid
G
Ml
Y
Hydrogen peroxide
Orthophenylamine diamine (OPD)
Solid
Mg
Y
Y
Y
NOTE: 1) EXPLOSIVE
2) OXIDIZING 3) FLAMMABLE 4) TOXIC 5) HARMFUL 6) CORROSIVE
7) IRRITANT 8) CARCINOGEN 9) MUTAGEN 10) TERATOGEN 11) DUST 12) INHALATION
13) INGESTION 14) SKIN ABSORPTION 15) SKIN OR EYE 16) INJECTION 17) MAXIMUM EXPOSURE LIMIT
18) OCCUPATIONAL EXPOSURE STANDARD 19) RADIATION 20) OTHER (specify)
3.
KNOWN
HAZARDS
(See Note;
use numbers)
5, (harmful)
7 (irritant)
5, (harmful)
7 (irritant)
5, (harmful)
7 (irritant)
5, (harmful)
7 (irritant)
5, (harmful)
7 (irritant)
5, (harmful)
7 (irritant)
5 (harmful)
7 (irritant)
7 (irritant)
2 (oxidising)
6 (corrosive)
4 (toxic)
5 (harmful)
7 (irritant)
8 (may cause
cancer)
Ref Note 17, 18: see EH40 (current edition).
PERSONNEL INVOLVED WITH SUBSTANCES (continue on a separate sheet if necessary)
NAME/CLASS
Dwek
4.
INITS
MV
STATUS
Supervisor /
Lecturer
INVOLVEMENT - DATE OF USE or
DAILY/WEEKLY/MONTHLY/OCCASIONALLY
Occassionally
OTHER GROUPS/PERSONS WHO MAY HAVE ACCESS TO THE SUBSTANCES
(e.g. students, cleaners, maintenance staff, contractors, visitors, storekeepers, etc.):
Stores
State control measures (to prevent inappropriate persons/groups having access to the substances ):
Store in locked cabinet with other corrosive / toxic materials. Access to named personnel only.
5.
Cabinet should be well ventilated.
EMERGENCY PROCEDURES (as per Hazard Data Sheet).
if any of the substances or procedures identified above is likely to pose a special hazard in an emergency, then identify below action to be taken.
CODE
Sodium bicarbonate
Sodium hydrogen carbonate
Sodium chloride (salt)
SPILLAGE/UNCONTROLLED RELEASE
Wear respirator, chemical safety goggles,
rubber boots and heavy rubber gloves.
Sweep up, place in bag and hold for waste
disposal Avoid raising dust and ventilate
area and wash spill site after material pick-up
is complete.
Ditto
Ditto
FIRE
Use a water spray, carbon dioxide or dry
chemical powder / foam. Emits toxic fumes
under fire conditions.
Ditto
Ditto
Potassium chloride
di-sodium hydrogen phosphate
Potassium dihydrogen
phosphate
Sulphuric acid
Citric acid
Hydrogen peroxide
Orthophenylamine diamine
(OPD)
Ditto
Ditto
Ditto
Ditto
Ditto
Ditto
Wear respirator, chemical safety goggles,
rubber boots and heavy rubber gloves.
Absorb on sand and place in closed
containers for disposal. Ventilate area and
wash spill site after material pick-up is
complete.
Wear respirator, chemical safety goggles,
rubber boots and heavy rubber gloves.
Sweep up, place in bag and hold for waste
disposal Avoid raising dust and ventilate
area and wash spill site after material pick-up
is complete.
Wear respirator, chemical safety goggles,
rubber boots and heavy rubber gloves.
Absorb on sand and place in closed
containers for disposal. Ventilate area and
wash spill site after material pick-up is
complete.
Wear respirator, chemical safety goggles,
rubber boots and heavy rubber gloves.
Sweep up, place in bag and hold for waste
disposal Avoid raising dust and ventilate
area and wash spill site after material pick-up
is complete.
Ditto
Ditto
Ditto
Dissolve or mix with a combustible solvent
and burn in a chemical incinerator equipped
with an afterburner and scrubber.
If personnel are affected (fumes, contamination, etc.) treatment to be adopted:
SPECIFY PER SUBSTANCE
(CODE AS PER HAZARD DATA SHEET)
sodium bicarbonate, sodium hydrogen carbonate, sodium chloride, potassium chloride, di sodium hydrogen phosphate, potassium dihydrogen
phosphate
If swallowed, wash out mouth with water provided if person is conscious. Call a physician.
If inhaled remove to fresh air. If breathing becomes difficult call a physican. In case of contact immediately wash skin with soap and copious quantities of water. In
case of contact with eyes, flush with copious quantities of water for at least 15 minutes. Assure adequate flushing by separating eyelids with fingers. Call a
physician.
Sulphuric acid, citric acid, hydrogen peroxide
If swallowed, wash out mouth with water provided if person is conscious. Call a physician.
If inhaled remove to fresh air. If breathing becomes difficult call a physican. In case of contact immediately wash skin with soap and copious quantities of water. In
case of contact with eyes, flush with copious quantities of water for at least 15 minutes. Assure adequate flushing by separating eyelids with fingers. Call a
physician.
Orthophenylamine diamine
In case of an accident or if you feel unwell, seek medical advice immediately. In case of contact with eyes rinse immediately with plenty of water and seek medical
advice.
(NB. Antidotes and special treatment may be obtained through...................................................................................................................................)
6.
CONTROL MEASURES TO BE ADOPTED (NB. Consider the following points, the appropriate Biosciences Safety Code plus any other
necessary measures. SPECIFY PER SUBSTANCE AS NECESSARY FOR EACH OF THE ITEMS BELOW.)
Always use TFA in fume cupboard, wear gloves (see COSHH form attached). Always add acid to water in small volumes NOT water to acid.
1. Limiting exposure time of personnel - state maximum time
2. Partial enclosure with local exhaust ventilation
3. Local exhaust ventilation
4. Sufficient general ventilation
5. Personal protective equipment (goggles/gloves/clothing/mask/breathing apparatus/safety screen, etc.) SPECIFY
* 6. Safe disposal/storage of hazardous substances
* 7. Regular cleaning of the workplace
* 8. Adequate washing facilities
* = MANDATORY
STORAGE - SAFETY CONSIDERATIONS
Sulphuric acid – toxic store cabinet
Citric acid – shelf of lab.
Hydrogen peroxide - fridge
HANDLING PRECAUTIONS
Always wear gloves and goggles.
Use in fume hood.
Orthophenylamine diamine - fridge
Wear gloves and avoid breathing dust
Sodium and potassium salts – lab shelf
Wear gloves and avoid breathing dust
DISPOSAL PROCEDURES DURING AND AT END OF EXPERIMENT
Dispose of plasticware after autoclaving via laboratory waste route. Dispose acid solutions via chemical disposal officer.
7.
REVIEW AND MONITORING OF CONTROL MEASURES
(Required checks, and their frequency, on the adequacy and maintenance of the control measures during the course of the experiment)
(NB. Biosciences Safety Code in respect of health surveillance needs. This form is not adequate if health surveillance is required.)
8.
OTHER RELEVANT INFORMATION (continue on a separate sheet if necessary)
(e.g. restriction of usage; transportation risks; labelling requirements; assessment review date, etc.)
9.
I certify that, to the best of my knowledge, the above precautions will adequately control the risks from the hazardous substances listed. I have brought the
assessment to the notice of the relevant technicians; academic staff; students; researchers (delete as necessary).
Name of Assessor:
Miriam Dwek
Signed:
Status of Assessor:
Lecturer
Date: