Activities in 2009
Classical swine fever
John Pasick
Canadian Food Inspection Agency, National Centre for Foreign Animal Disease
1015 Arlington Street, Winnipeg, Manitoba, Canada R3E 3M4
Tel.: (1-204) 789-2013, Fax: (1-204) 789-2038
e-mail address: John.Pasick@inspection.gc.ca
Summary of general activities related to the disease
1.
Test(s) in use/or available for the specified disease at your laboratory
Test
Detection
Target
Purpose
Totals
Regulatory
Disease
Investigation
Research
Quality
Assurance
ELISA
Antibody
7237
39
0
408
7684
NPLA
Antibody
0
26
0
132
158
IFAT
Antigen
0
0
0
0
0
Virus Isolation
(PK-15 cells) &
IPA
Antigen
0
232
0
350
582
Real-time RTPCR
Nucleic Acid
0
17
0
233
250
Conventional
RT-PCR
Nucleic Acid
0
13
0
128
141
Nucleic Acid
Sequencing
5’-NTR and E2
gene
0
0
10
24
34
2.
Production and distribution of diagnostic reagents
NCFAD produces the following reagents:
•
CSFV, BVDV and BDV stocks
•
CSFV hyperimmune serum
•
Timed antisera to various CSFV isolates
•
In vitro transcribed CSFV, BVDV and BDV 5’-NTR RNA for use in PCR proficiency panels
•
Fluorescent conjugates of porcine anti-CSFV IgG
•
CSFV specific monoclonal antibodies
•
Baculovirus expressed recombinant E2 proteins
Annual reports of OIE Reference Laboratories and Collaborating Centres, 2009
1
Classical swine fever
The following amounts were supplied nationally and for use by our own laboratory
•
145 real-time CSF RT-PCR proficiency panels
NCFAD has not supplied any reagents to OIE member countries in 2009.
Activities specifically related to the mandate
of OIE Reference Laboratories
3.
International harmonisation and standardisation of methods for diagnostic testing or the
production and testing of vaccines
NCFAD participates in the Inter-Laboratory Comparison Test organized by the EU Reference Laboratory for
Classical Swine Fever in Hannover, Germany
4.
Preparation and supply of international reference standards for diagnostic tests or vaccines
None.
5.
Research and development of new procedures for diagnosis and control
Work continues on the development of serological tests for CSF using CSFV-specific monoclonal antibodies and
recombinant E2 proteins that have been produced in house.
6.
Collection, analysis and dissemination of epizootiological data relevant to international disease
control
None.
7.
Provision of consultant expertise to OIE or to OIE Members
None.
8.
Provision of scientific and technical training to personnel from other OIE Members
One scientist from the Estados Unidos para la Prevención de la Fiebre Aftosa y Otras Enfermedades Exóticas de
los Animales, Mexico was trained in CSF diagnostic tests that included the neutralisation peroxidase-linked assay,
virus isolation, immunoperoxidase assay, conventional RT-PCR and real-time RT-PCR.
9.
Provision of diagnostic testing facilities to other OIE Members
None.
10. Organisation of international scientific meetings on behalf of OIE or other international bodies
None.
11. Participation in international scientific collaborative studies
A collaborative study with the Laboratory of Virology, Faculty of Veterinary Medicine, San Marcos University,
Peru was carried out to molecularly characterize CSF viruses that were isolated from domestic pigs from different
regions of Peru in 2008. All virus isolates were found to belong to genetic sub-group 1.1 consistent with the sub-
2
Annual reports of OIE Reference Laboratories and Collaborating Centres, 2009
Classical swine fever
group of viruses that have been identified from other South American countries. Although the Peruvian isolates
are most closely related to viruses from Colombia and Brazil, they form a monophyletic clade, which suggests
they have a distinct evolutionary history.
12. Publication and dissemination of information relevant to the work of OIE (including list of
scientific publications, internet publishing activities, presentations at international conferences)

Presentations at international conferences and meetings
None.
Scientific publications in peer-reviewed journals
None.

Other communications
None.
13. Inscription of diagnostic kits on the OIE Register
i)
Did you participate in expert panels for the validation of candidate kits for inscription on the
OIE Register? If yes, for which kits?
No.
ii)
Did you submit to the OIE candidate kits for inscription on the OIE Register? If yes, for
which kits?
No.
_______________
Annual reports of OIE Reference Laboratories and Collaborating Centres, 2009
3