Transcription
Supplemental Instruction
Iowa State University
Leader:
Course:
Instructor:
Date:
Lilli Howard
BIOL/GEN 313
Dr. Myers
01/30/14
1. Which of the following is not a type of RNA found in prokaryotes?
a. tRNA
b. miRNA
c. rRNA
d. mRNA
2. T/F -Transcription uses a template RNA strand
3. T/F -RNA is synthesized in the 3' → 5' direction
4. Does transcription transcribe the entire genome? What specifically does it transcribe?
5. Label the diagram below with: template/nontemplate strands, promoter, terminator,
transcription start/stop sites, coding region, 5' end, 3' end
a. Draw and label the transcript
6. What is a promoter sequence? Where are they found? What are two examples found in
prokaryotes?
7. Describe the two kinds of transcription termination. What is the difference between
protein dependent and independent termination?
8. Why is RNA less stable than DNA?
1060 Hixson-Lied Student Success Center  515-294-6624  sistaff@iastate.edu  http://www.si.iastate.edu
1. Which of the following statements about transcription is TRUE?
a) Only discrete segments of the DNA genome are transcribed into RNA
b) Transcription utilizes deoxyribonucleotide triphosphates
c) Transcription requires a pre-existing nucleic acid polymer
d) Transcription is the same in all cells in a multicellular organism
e) Transcription cannot occur within euchromatic regions of chromosomes
2. Which component of the DNA replication machinery most closely resembles RNA
polymerase?
a) primase
b) helicase
c) DNA polymerase I
d) DNA polymerase III
e) telomerase
3. If the sequence of an RNA molecule is 5’-GGCAUCGACG-3’, what is the sequence of the
corresponding nontemplate strand of DNA?
a) 5’-GGCATCGACG-3’
b) 3’-GGCATCGACG-5’
c) 5’-CCGTAGCTGC-3’
d) 3’-CCGTAGCTGC-5’
4. What would be the effect of a mutation in a prokaryotic promoter that changes a base within
the -35 consensus sequence so that RNA polymerase bound to the sequence less tightly than
normal ?
a) Transcription would initiate more frequently than normal
b) Transcription would initiate less frequently than normal
c) Transcription would initiate normally but would terminate prematurely
d) Sigma factor would no longer be required for specific initiation at that promoter
e) Transcription elongation would occur at a slower rate than normal
5. Which component of the DNA replication machinery is the last one to act?
a) helicase
b) DNA gyrase
c) single-stranded DNA binding protein
d) DNA ligase
e) DNA polymerase I
6. Which statement is NOT true of heterochromatin?
a) Heterochromatin is present all over the inactive X chromosomes in female mammals.
b) Heterochromatin contains genes that are transcribed at high levels.
c) Heterochromatin is present on most of the Y chromosomes of male mammals.
d) Heterochromatin remains highly condensed throughout the cell division cycle.
e) Heterochromatin is present at centromeres and telomeres.
1060 Hixson-Lied Student Success Center  515-294-6624  sistaff@iastate.edu  http://www.si.iastate.edu