Prokaryotic Transcription
Supplemental Instruction
Iowa State University
Leader:
Course:
Instructor:
Date:
Lilli Howard
BIOL/GEN 313
Dr. Rodermel/Dr. Tuggle
09/29/14
1. What are the three basic stage of transcription? Describe what happens at each stage.
2. ____________ binding is responsible for determining how frequently a gene is
transcribed.
2. T/F -Transcription uses a template RNA strand
3. T/F -RNA is synthesized in the 3' → 5' direction
4. Does transcription transcribe the entire genome? What specifically does it transcribe?
5. Label the diagram below with: template/nontemplate strands, promoter, terminator,
transcription start/stop sites, coding region, 5' end, 3' end, +1
a. Draw and label the transcript
6. What is a promoter sequence? Where are they found? What are two examples found in
prokaryotes?
7. Describe the two kinds of transcription termination found in prokaryotes. What is the
difference between protein dependent and independent termination?
1060 Hixson-Lied Student Success Center  515-294-6624  sistaff@iastate.edu  http://www.si.iastate.edu
8. What is polycistronic RNA?
9. Which of the following statements about transcription is TRUE?
a) Only discrete segments of the DNA genome are transcribed into RNA
b) Transcription utilizes deoxyribonucleotide triphosphates
c) Transcription requires a pre-existing nucleic acid polymer
d) Transcription is the same in all cells in a multicellular organism
e) Transcription cannot occur within euchromatic regions of chromosomes
10. If the sequence of an RNA molecule is 5’-GGCAUCGACG-3’, what is the sequence of
the corresponding non-template strand of DNA?
a) 5’-GGCATCGACG-3’
b) 3’-GGCATCGACG-5’
c) 5’-CCGTAGCTGC-3’
d) 3’-CCGTAGCTGC-5’
11. Which statement is NOT true of heterochromatin?
a) Heterochromatin is present all over the inactive X chromosomes in female mammals.
b) Heterochromatin contains genes that are transcribed at high levels.
c) Heterochromatin is present on most of the Y chromosomes of male mammals.
d) Heterochromatin remains highly condensed throughout the cell division cycle.
12. What binds to the -10 consensus sequence found in most bacterial promoters?
a) The holoenzyme
b) The sigma factor alone
c) The core enzyme alone
d) mRNA
13. Which of the following phrases does not describe a function of the promoter?
a) Serves as sequence to which transcription apparatus binds
b) Determines the first nucleotide that is transcribed into RNA
c) Determines which DNA strand is template
d) Signals where transcription ends
14. Which of the following is not a type of RNA found in prokaryotes?
a) tRNA
b) miRNA
c) rRNA
d) mRNA
1060 Hixson-Lied Student Success Center  515-294-6624  sistaff@iastate.edu  http://www.si.iastate.edu