Biological Resource Center
National Institute of Technology and Evaluation
独立行政法人製品評価技術基盤機構
バイオテクノロジーセンター
2-5-8, Kazusakamatari, Kisarazu, Chiba 292-0818, JAPAN
Tel: +81-438-20-5763 Fax: +81-438-52-2329
E-mail: nbrc@nite.go.jp
〒292-0818 千葉県木更津市かずさ鎌足 2-5-8
Tel: 0438-20-5763 Fax: 0438-52-2329
E-mail: nbrc@nite.go.jp
ACCESSION FORM
(Clones)
For NBRC use only
NBRC No:
Medium No.
List: Open □ Close□
Accepted date:
Keeper:
Restriction code:
We ask the depositor to supply data on the following form (Please print or type).
1. Clone name/designation:
SPABC
2. Clone description:
a) Name or locus symbol(s) and origin(s)
XXSPB
b) Total size (bp or kb): 4,341bp
c) DDBJ /EMBL /Gen Bank accession number of the plasmid: M99999
d) Vector (name, size/coordinates and DDBJ /EMBL/GenBank accession number:
pUC118/3,162bp/1-17 and 754-2932/U07649
e) Insert (gene name(s)/source(s), size(s) /coordinates and DDBJ /EMBL/GenBank accession number):
gene name: surfactant-associated protein 9999
source: Homo sapiens (human)
size: 1,179bp
accession number: J99999
3. Form of deposit:
DNA dissolved in TE (pH8.0)
4. Host organism name (if supplied in host) or DNA concentration:
100 microgram/ml
5. Other DNA and/or collection number:
ATCC99999
6. Deposited by (name): Taro Kitaku
Date:
2010/11/26
Institution: Biological Resource Institute
Address: 2-5-8 Kazusakamatari, Kisarazu-shi, Chiba Pref. 292-0818, Japan
Tel: +81-428-52-XXXX
Fax: +81-428-52-XXXX
E-mail: kitaku-taro@xxx.go.jp
7. Constructed by (name): Jiro Kochiku
Institution: Molecular Biology Institute
Date: 2001/10/24
8. Isolated by (name): Saburo Bunri
Institution: Molecular Biology Institute
Date: 2001/10/01
9. History since original isolation:
NBRC <-- Depositor <-- Saburo Bunri
10. References for this DNA (please attach detailed descriptions of the plasmid, e.g. a restriction map and of the
construction methods, and references concerning source DNAs):
White BB et al. Isolation and characterization of the human DNA gene. Nature 999: 361-363, 1999
PubMed: 9999
11.Comments (mRNA size(s) detected, gene mutations, expression information, etc):
Restriction digests of the clone give the following sizes (kb): BglI--1.5, 1.2, 1.6; EcoRI--2.8, 1.5;
HindIII--3.2,1.1; KpnI--4.0, 0.3; PstI--3.6, 0.3 (doublet).
Contains the complete coding sequence except for codons for the first two amino acids. The 3' end is the
naturally occurring EcoRI site in the 3' untranslated region at nt 4,243 of the genomic sequence record.
12.Safety information
a). If supplied in host, is this strain known to be or likely to be pathogenic?
Yes
(human
, animal
, plant
), No
,
Unknown
If yes, please specify details.
Biosafety level
Level 1
Level of physical containment: P1
,
,
Level 2
P2
,
, Level 3
P3
b). Does this clone contain a part of and/or full-length genes that express pathogenic traits?
Yes
(human , animal , plant ),
No
,
Unknown
If yes, please specify the pathogenic traits.
13.Method for amplification:
PCR
Growth as bacteriophage*
Cultivation of host*
Other (please specify)
Recommended host strain and appropriate growth conditions:
Host strain: Escherichia coli HB101 and Escherichia coli BMH71-18 mutS
Growth conditions: LB Medium (ATCC medium 1065) with 50 microgram/ml ampicillin; Temperature: 37℃
I,
Taro Kitaku
, hereby certify that all the answers and information provided above have been
written by myself or under my direction, and are true to my own knowledge. In addition, I hereby confirm that I have read
the attached AGREEMENT FOR DEPOSITORY OF BIOLOGICAL RESOURCES carefully and hereby express my consent
to all the terms and conditions set forth in the attached AGREEMENT FOR DEPOSITORY OF BIOLOGICAL RESOURCES.
Taro Kitaku
Signature of Depositor
October 1, 2010
Date
Domestic use only. 国内の寄託者は下欄に日本語でご記入下さい。
氏名: 寄託 太郎
所属機関: 生物資源機関
住所: 〒292-0818 千葉県木更津市かずさ鎌足 2-5-8
(April, 2011)