Supplementary information
for
Identification of proteins involved in membrane fouling in
membrane bioreactors (MBRs) treating municipal wastewater
Taro Miyoshi1,*, Tomoyasu Aizawa2, Katsuki Kimura3, Yoshimasa Watanabe1
1: Research Center for Environmental Nano and Bio Engineering, Hokkaido University,
N13W8, Kita-ku, Sapporo 060-8628, Japan
2: Division of Biological Sciences, Hokkaido University, N10W8, Kita-ku, Sapporo
060-0810, Japan
3: Division of Built Environment, Hokkaido University, N13W8, Kita-ku, Sapporo
060-8628, Japan
2 Pages, 1 Figure and 1 Table
*Corresponding author
Tel & Fax: +81-11-706-6267
E-mail: jms-mbr@eng.hokudai.ac.jp
Fig. S1 SDS-PAGE gel of foulant obtained from MBR 3 fractionated by a sequential
precipitation with ethanol and TCA. Lanes: lane M, molecular weight markers; lane 0,
proteins that can be separated without addition of ethanol; lane 25, proteins separated
with 25% (v/v) ethanol; lane 50, proteins separated with 50% (v/v) ethanol; lane 80,
proteins separated with 80 (v/v) ethanol; lane T, proteins separated with 80% (v/v)
ethanol and 10% (w/v) TCA.
Table S1 Results of N-terminal amino acid sequencing analysis
Protein
1
2
Amino acid sequence
Ala Thr Val Ile
Gln Gln Gly Val
Ala Leu Thr Phe
Ala Thr Val Ile
Gln Gln Gly Val
Ala Leu Thr Phe
Leu Asp Gly Val
Val Ala Asp Gly
Ser Glu Asn Leu
Leu Asp Gly Val
Val Ala Asp Gly
Ser
x Asn Leu
Thr Lys
Ile Gly
Thr Lys
Ile
x