GenScript Corporation
Lambda
DNA/Eco911(BstEII) Marker
Product Name:
Lambda DNA/ Eco911 (BstEII) Marker
Catalog:
MM1222
Size:
50ug
Supplied with 6 x Loading dye solution.
Description:
Lambda DNA was completely digested by Eco911, phenol extracted, ethanol precipitated and
dissolved in 10mM Tris-HCl (pH 7.6) and 1mM EDTA. The marker yields the following 14 discrete
fragments (in base pairs): 8453, 7242, 6369, 5687, 4822, 4324, 3675, 2323, 1929, 1371, 1264, 702,
224, 117.
Storage Buffer: 10mM Tris-HCl, pH 7.6. 1mM EDTA
6 x loading buffer: 60% Glycerol, 0.09% bromophenol blue, 0.09% xylene cyanol F and 60mM
EDTA.
Concentration: 0.05mg DNA/ml
Storage: -20C
Quality Control:
Agarose gel electrophoretric analysis of homogeneity of Eco911 fragmentation patterns.
Recommendations for use
Vortex gently just prior to use.
Prepare DNA Marker, 15, before loading as following:
1ul (0.5ug) of Marker, 15
1ul of 6xLoading Dye Solution
4ul of deionized water.
The cohesive ends of the 12nt cos site of bacteriophage lambda from fragments of 8453bp and
5687bp( indicated ) may anneal and form an additional band of 14140bp. These fragments can
be separated by heating at 65oC for 5 min and then cooling on ice for 3min.
Following electrophoretic separation the DNA bands can be visualized by ethidium bromide
staining.
Apply 0.1ug( 0.2ul ) of DNA Marker, 15, per 1mm of agarose gel lane width.
GenScript Corporation
Tel: 732-885-9188
Fax: 732-210-0262
www.genscript.com
email: info@genscript.com
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