・DNA Step Ladders
・OneSTEP Ladders
・Ladders for 10minute separation
・Wide Range Ladders
・Markers ＆ OneSTEP Markers
・DGGE Markers
・PCR Marker Makers
Size St
Standard
Markers
d
・RNA Si
・RNA Step Ladder
・Loading Buffers
Molecular-Weight
Markers
・Prestain markers
・Western marker
・Biotinylated marker
・Markers for CBB dyeing
・Easy SeparatorTM
・Negative Gel Stain MS Kit
・SuperSepTM Ace
・SuperSepTM DNA
DNA Step Ladder (1~6)
Ready With dye and glycerol already
to
added, it is ready to be applied to
use the agarose gel.
for low-molecular-range analysis.
100
90
80
70
60
50
40
30
20
10
6xBlue/
Orange
Loading
Dye
1% Agarose
Loading:
1µL/lane
1
（bp） −20℃
300
275
250
225
200
175
150
125
100 Loading:
5µL/lane
75
50
25
−20℃
6x
Loading
Dye
Loading:
5µL/lane
2% Agarose
（bp）
−20℃
25 bp DNA Step
Ladder (25-500 bp)
2
3
50 bp DNA Step
Ladder (50-800 bp)
（bp）
（bp）
（ng） −20℃
500 60
400 60
300 60
6xBlue/
Orange
250 60
Loading
200 120
Dye
175 80
150 80
125 80
Loading:
100 120
5µL/lane
75 80
50 80
25 80
800
700
600
500
400
300
200
150
100
50
2% Agarose
25 bp DNA Step
Ladder (25-300 bp)
3.5% Agarose
10 bp DNA Step
Ladder (10-100 bp)
4
OneSTEP Ladder (7~12)
for simultaneous measurement of DNA size and amount.
5% Agarose 21
6x
Loading
Dye
5
717
600
500
400
6
Loading:
5-10µL/lane
OneSTEP Ladder 500
(0.5-5 kbp)
（bp）
5,000
4,500
4,000
3,500
3,000
2,500
2,000
Ready
to
use
6x
Loading
Dye
286
200
100
7
Loading:
5µL/lane
OneSTEP Ladder 1 kb
(1-10 kbp)
9
each fragment:
50ng/5µL
1% Agarose S
1,000
500
10
8
Concentration:
0.69µg/5µL
（kbp）
Ready
to
use
10.0
9.0
8.0
7.0
6.0
5.0
4.0
3.0
1,500
50 100
OneSTEP Ladder
Supercoiled Plasmid
（kbp）
Ready
to
use
Ready
to
use
Gene Ladder 100
(0.1-2 kbp)
（bp）
（ng/5µL）
Ready
to
use
2,000 100
1,500 75
10.0
9.0
8.0
7.0
6.0
5.0
−20℃
4.0
2.0
1.0
11
each fragment:
50ng/5µL
2
each fragment:
30ng/5µL
（bp）
（ng/5µL）
2,000 100
1,800 90
1,600 80
1,400 70
1,200 60
1,000 100
900 45
800 40
700 35
600 30
500 50
400 20
300 15
200 10
100 10
Concentration:
0.755µg/5µL
2% Agarose S
100
75
50
35
25
20
15
10
OneSTEP Ladder 100
(0.1-2 kbp)
（bp）
（ng/5µL）
2,000 100
1,500 75
1,000 50
900 45
800 40
700 35
600 30
500 75
400 22.5
450 20
350 17.5
300 15
250 37.5
200 10
150 7.5
100 10
Ready
to
use
3% Agarose 21
200
150
−20℃
（bp）
1,500
1,000
900
800
−20℃
1% Agarose S
（bp）
300
Ready
to
use
OneSTEP Ladder 50
(0.05-2 kbp)
3% Agarose 21
100 bp DNA Step Ladder
(0.1-1.5 kbp)
2% Agarose
DNA Step Ladder
(10-300 bp)
1% Agarose S
DNA Ladder
DNA Ladder
3.0
12
1,000
900
800
700
600
500
400
300
200
100
Concentration:
0.64µg/5µL
50
45
40
35
30
75
40
30
20
100
Ladder for 10minute separation (13~18)
（bp）
Ready
to
use
−20℃
（bp）
Ready
to
use
−20℃
200
100
6x
Loading
Dye
50
（bp）
Ready
to
use
−20℃
5,000
2,000
1,500
6x
Loading
Dye
850
6x
Loading
Dye
850
DNA Step Ladder
(500-10,000bp)
6x
Loading
Dye
Loading:
20µL/lane
14
100
25
25
25
25
0.1µg/5µL
Loading:
20µL/lane
15
2,000
1,000
500
100
17 Concentration:
2% Agarose
2% Agarose
50
（bp）
（ng/5µL）
1,000
500
200
Ready
to
use
10,000
4,000
2,000
−20℃
400
400
10
2% Agarose
4% Agarose 21
Gene Ladder Fast 1
(4 bands:0.1-2 kbp)
（bp）
Ready
to
use
20
Loading:
20µL/lane
13
DNA Step Ladder
(100-5,000 bp)
1% Agarose S
DNA Step Ladder
(50-1,500 bp)
Loading:
20µL/lane
16
Gene Ladder Fast 2
(6 bands:0.1-10 kbp)
Ready
to
use
1% Agarose S
DNA Step Ladder
(10-200 bp)
−20℃
: Store at ｰ20℃
NO DISPLAY: Store at 2〜10℃
（bp）
（ng/5µL）
10,000
4,000
2,000
1,000
500
100
25
25
25
25
25
25
18
Concentration:
0.15µg/5µL
Number
Product name
Code No.
Package Size
1
10 bp DNA Step Ladder (10-100 bp)
040-28721
32.5 μg (50 μL)
2
25 bp DNA Step Ladder (25-300 bp)
292-69001
100 μg
3
25 bp DNA Step Ladder (25-500 bp)
044-30711
500 μL
4
50 bp DNA Step Ladder (50-800 bp)
544-02311
85 μg (250 μL)
5
DNA Step Ladder（10-300 bp）
298-66301
50 μg
6
100 bp DNA Step Ladder（0.1-1.5 kbp）
296-68801
30 μg
7
8
9
OneSTEP Ladder 50（0.05-2.0 kbp）
OneSTEP Ladder 100（0.1-2 kbp）
OneSTEP Ladder 500 (0.5-5 kbp)
319-06044
25 μL
315-06041
500 μL
311-06043
500 μL ×2
317-05244
25 μL
313-05241
500 μL
319-05243
500 μL ×2
317-05364
25 μL
313-05361
500 μL
319-05363
500 μL × 3
314-05374
25 μL
310-05371
500 μL
10
OneSTEP Ladder 1 kb（1-10 kbp）
316-05373
500 μL × 3
11
OneSTEP Ladder Supercoiled Plasmid
317-05381
500 μL
12
Gene Ladder 100（0.1-2 kbp）
316-06951
500 μL
13
DNA Step Ladder（10-200 bp）
, Fast Separation Type
292-66201
10 μg（0.5mL）× 2
14
DNA Step Ladder（50-1,500 bp）
, Fast Separation Type
290-61001
10 μg（0.5mL）× 2
15
DNA Step Ladder（100-5,000 bp）
, Fast Separation Type
296-61101
10 μg（0.5mL）× 2
16
DNA Step Ladder（500-10,000 bp）
, Fast Separation Type
292-61201
10 μg（0.5mL）× 2
17
Gene Ladder Fast 1
（4 bands:0.1-2 kbp）
317-06981
500 μＬ× 2
18
Gene Ladder Fast 2（6 bands:0.1-10 kbp）
314-06991
500 μＬ× 2
Note
: Nippon Geneʼs products
3
DNA Ladder
for wide-range and simultaneous analysis
l i off b
both
th DNA size
i and
d amount.
t
Marker
1,500
32
1,030
900
800
700
600
500
400
300
200
100
80
200
180
160
140
120
200
80
60
40
20
16
−20℃
20
（bp）
（ng/5µL）
Ready
to
use
20,000 100
10,000 50
7,000 35
5,000 75
4,000 40
3,000 30
（bp）
（ng/5µL）
Ready
to
use
2,000 100
1,500 30
1,300 26
1,000 100
21
700 35
500 75
400 40
300 30
200 20
100 100
& the OneSTEP Marker
−20℃
Gene Ladder Wide 2
(0.1-20 kbp)
Ready
to
use
22
1% Agarose
1% Agarose
6x
Loading
Dye
Gene Ladder Wide 1
(0.1-20 kbp)
（bp）
（ng/5µL）
10,000 100
8,000 80
6,000 60
5,000 50
4,000 40
3,000 30
2,500 25
2,000 20
1,500 15
1,000 100
800 80
600 60
400 40
200 20
Ready
to
use
1% Agarose S
（bp）（ng/20µL）
10,000 200
8,000 160
6,000 120
5,000 100
4,000 80
3,000 60
2,500 50
2,000 40
−20℃
19
Smart Ladder
(0.2-10 kbp)
1% Agarose
DNA Step Ladder Mix
(0.08-10 kbp)
20,000
10,000
7,000
5,000
4,000
3,000
100
50
35
75
40
30
2,000
1,500
1,000
900
800
700
600
500
400
200
100
100
30
50
45
40
35
30
75
40
20
100
(23~33)
DNA molec
molecular-weight
lar eight markers that set si
size
e standards for linear do
double-strand
ble strand DNA
DNA. E
Each One-Step Marker
comes with pre-added dye and glycerol, so they can be applied directly to the agarose gel.
Marker 2
(λ/Hind Ⅲ・EcoRⅠ)
（kbp）
23.13
（kbp）
21.23
9.42
6.56
5.15
4.36
4.27
Marker 3
(λ/Hind Ⅲ+λ/EcoRⅠ)
4.98
3.52
2.02
Marker 5
(φX174/Hinc Ⅱ)
1.38
0.95
25
0.83
Marker 6
(λ/StyⅠ)
（bp）
1,057
770
612
495
392
345
341
335
297
291
210
162
（kbp）
19.33
（bp）
1,353
1,078
872
603
26
（kbp）
（kbp）
165.65
56.74
165.65
50.31
41.77
7.74
6.22
23.45
21.10
17.70
4.26
3.47
2.69
1.88
28
1.49
0.93
29
4
310
281
271
234
194
118
72
Marker 8GT
(T4 GT7 + T4 GT7/Bgl Ⅱ)
10.06
30
0.3% Agarose H
79
（kbp）
23.13
21.23
9.42
7.42
6.56
5.81
5.65
4.88
4.36
3.52
2.32
2.02
Marker 7GT
(T4 GT7 + T4 GT7/BglⅠ)
0.3% Agarose H
27
1.58
1% Agarose S
24
1% Agarose S
1.91
1% Agaose S
23
1% Agarose S
2.32
2.02
Marker 4
(φX174/Hae Ⅲ)
3% Agarose 21
Marker 1
(λ/Hind Ⅲ)
3% Agarose 21
DNA Ladder
Wide Range Ladder
der (19~22)
17.82
17.70
15.71
14.28
10.65
8.03
5.59
5.51
Marker 10
(pBR322/MspⅠ)
Number
32
Marker 11
(pUC19/MspⅠ)
（bp）
622
527
404
307
242
238
217
201
190
180
160
160
147
147
123
110
90
76
67
（bp）
33
Product name
19
DNA Step Ladder Mix（0.08-10 kbp）
20
Smart Ladder (0.2-10 kbp)
21
22
Code No.
Package Size
292-68401
1 mL
25 μL
Gene Ladder Wide 1（0.1-20 kbp）
313-06961
500 μL × 2
Gene Ladder Wide 2（0.1-20 kbp）
310-06971
OneSTEP Marker 2
（lambda/
dⅢ+
d Ⅲ digest）
Marker 2
（lambda/
dⅢ+
d Ⅲ digest）
R I double digest）
R I double digest）
OneSTEP Marker 3
（lambda/
d Ⅲ + lambda/
Marker 3
（lambda/
d Ⅲ + lambda/
R I digest mixture）
R I digest mixture）
Ⅲ digest）
26
Marker 4（phiX174/
c Ⅱ digest）
Ⅰdigest）
Marker 6（lambda/
Ⅰdigest）
Marker 7 GT
Marker 8 GT
OneSTEP Marker 9（phiX174/
Marker 9（phiX174/
fⅠdigest）
fⅠdigest）
80 μg
315-00566
80 μg × 5
314-05271
1500 μL
310-05273
1500 μL × 5
316-00574
80 μg
312-00576
80 μg × 5
318-05791
375 μL
314-05793
375 μL × 5
Ⅰdigest）
317-05803
375 μL × 5
OneSTEP Marker 11（pUC19/
Ⅰdigest）
Ⅰdigest）
15 μg
15 μg × 5
311-05281
1500 μL
317-05283
1500 μL × 5
313-00964
80 μg
319-00966
80 μg × 5
315-03981
10 μg
311-03983
10 μg × 5
312-03991
10 μg
10 μg × 5
318-05811
375 μL
314-05813
375 μL × 5
15 μg
15 μg × 5
315-05821
375 μL
311-05823
375 μL × 5
313-01503
33
15 μg × 5
375 μL
317-01501
Ⅰdigest）
15 μg
311-05801
310-01493
OneSTEP Marker 10（pBR322/
Marker 11（pUC19/
1500 μL × 5
319-00564
314-01491
32
Marker 10（pBR322/
311-05264
318-03993
31
120 μg × 5
1500 μL
318-00676
28
120 μg
317-05261
312-00674
c Ⅱ digest）
OneSTEP Marker 6（lambda/
1500 μL × 5
311-00666
27
Marker 5（phiX174/
314-05254
315-00664
Ⅲ digest）
OneSTEP Marker 5（phiX174/
1500 μL
312-00456
Note
500 μL × 2
310-05251
316-00454
OneSTEP Marker 4（phiX174/
30
OneSTEP Marker：
Store at 2〜10℃
500 μL
Marker 1（lambda/
29
−20℃
317-03941
OneSTEP Marker 1（lambda/
25
Storage conditions
DNA MW Marker：
313-03943
23
24
501
489
404
331
242
190
147
111
110
3% Agarose 21
（bp）
726
713
553
500
427
417
413
311
249
200
151
140
118
100
82
66
66
48
3% Agarose 21
31
3% Agarose 21
Marker 9
(φX174/HinfⅠ)
15 μg
15 μg × 5
312-05831
375 μL
318-05833
375 μL × 5
319-03261
15 μg
315-03263
15 μg × 5
: Nippon Geneʼs products
5
DNA Ladder
DNA markers for DGGE (dena
(denaturing
aturing grad
gradient gel electrophoresis)
electrophoresis). A CG clamp is a
added at the end of one side of
each fragment to enable separation / inspection by DGGE.
1
2
3
34
65%
5
35
65%
8% Acrylamide
4
1
2
3
4
5
6
7
8
9
10
DGGE Marker Ⅳ
(8 fragments)
Denaturant
50%
−20℃
36
70%
Denaturant
20%
−20℃
1
2
3
4
5
6
7
8
9
10
37
45%
No. of fragments (Conc.)
Application
5 (50ng/5µL)
For bacterial 16S rDNA
10 (100ng/5µL)
For bacterial 16S rDNA
10 (100ng/5µL)
For bacterial 16S rDNA
Constant voltage
Electrophoresis time, Temp.
120V
8 hours, 60℃
120V
8 hours, 60℃
50V
18 hours, 58℃
PCR Marker Maker (39~42)
DGGE Marker Ⅴ
(9 fragments)
Denaturant
20%
−20℃
1
2
3
4
5
6
7
8
38 50%
1
2
3
4
5
6
7
8
9
6% Acrylamide
Denaturant
35%
−20℃
DGGE Marker Ⅲ
(10 fragments)
7% Acrylamide
Denaturant
35%
−20℃
DGGE Marker Ⅱ
(10 fragments)
6% Acrylamide
DGGE MarkerⅠ
(5 fragments)
8% Acrylamide
DNA Ladder
4~38)
DGGE Marker (34~38
8 (80ng/5µL)
9 (50ng/5µL)
For fungal bacteria16S rDNA For fungal bacteria 16S rDNA
50V
20 hours, 60℃
75V
16 hours, 60℃
−20℃
for preparing DNA size markers
markers. Consisting of a mixt
mixture of template DNA, primer, and reaction buffer, the reagent
initiates a PCR with the simple addition of a Tag DNA polymerase “Gene Tag.” It can also be used as a positive
control while the PCR is under way.
（bp）
1057
770
612
495
392
345
341
335
297
291
210
162
79
Number
34
35
36
37
38
39
40
41
42
M
39
40
41
42
43
（bp）
0.5 µg
M：Marker 5（φX174/
c Ⅱ）
3 µL
39：100L（101 bp, 200 bp, 299 bp, 398 bp, 502 bp）
3 µL
40：100H（601 bp, 702 bp, 800 bp, 906 bp, 1,005 bp）
3 µL
41：200U（101 bp, 299 bp, 502 bp, 702 bp, 906 bp）
3 µL
42：200E（200bp, 398 bp, 601 bp, 805 bp, 1,005 bp）
3 µL each
43：Mixture of products from a 100L & 100H PCR
1005
905
800
702
601
502
398
299
200
101
Product name
DGGE MarkerⅠ(5 fragments)
DGGE Marker Ⅱ (10 fragments)
DGGE Marker Ⅲ (10 fragments)
DGGE Marker Ⅳ (8 fragments)
DGGE Marker Ⅴ (9 fragments)
PCR Marker Maker 100L
PCR Marker Maker 100L, Gene
attached
PCR Marker Maker 100H
PCR Marker Maker 100H, Gene
attached
PCR Marker Maker 200U
PCR Marker Maker 200U, Gene
attached
PCR Marker Maker 200E
PCR Marker Maker 200E, Gene
attached
Code No.
318-06411
Package Size
30 μL（for 6 applications）
314-06413
150 μL（for 30 applications）
312-06414
150 μL × 5（for 150 applications）
311-06401
30 μL（for 6 applications）
317-06403
150 μL（for 30 applications）
315-06404
150 μL × 5（for 150 applications）
315-06921
30 μL（for 6 applications）
311-06923
150 μL（for 30 applications）
319-06924
150 μL × 5（for 150 applications）
312-06931
30 μL（for 6 applications）
318-06933
150 μL（for 30 applications）
316-06934
150 μL × 5（for 150 applications）
319-06941
30 μL（for 6 applications）
315-06943
150 μL（for 30 applications）
313-06944
150 μL × 5（for 150 applications）
317-03061
For 10 applications
314-03071
For 10 applications
311-03081
For 10 applications
318-03091
For 10 applications
318-03111
For 10 applications
315-03121
For 10 applications
312-03131
For 10 applications
319-03141
For 10 applications
Note
: Nippon Geneʼs products
6
RNA Ladder
RNA Ladder
RNA Size Standard Marker (1~4)
RNA molecular-weight markers prepared by in vitro transcription.
（k base）
9
−70℃
（base）
−70℃
1% formaldehyde Agarose
6
5
4
6,583
4,981
3,638
300
2,604
1.5
1
2
200
100
3
RNA Ladder
(0.125-6 kb)
（base）
−80℃
1,000
750
500
400
5% polyacrylamide-urea
1
3
2.5
2
0.5
RNA Size Standard
Marker Ⅳ (0.28-6.58 kb)
（base）
−70℃
6,000
4,000
3,000
2,000
1,500
1,000
500
250
125
1% formaldehyde Agarose
RNA Size Standard
Marker Ⅲ (0.1-1 kb)
1,908
1% formaldehyde Agarose
RNA Size Standard
Marker Ⅱ
1,383
955
623
281
4
RNA Step Ladder (0.1–1 kb) (5)
Loading Buffer
an RNA molecular-weight
l
l
i ht marker
k can b
be di
directly
tl applied
li d
to native gel, denatured gel, or polyacrylamide gel.
A sample can be easily applied during DNA gel
electrophoresis to effectively predict the migration
point.
a
b
c
d
（Store at room temp.）
e
f
a
b
c
d
e
f
RNA Step Ladder (0.1-1 kb)
Xylene Cyanol FF
（base）
2X
Loading
Dye
1,000
800
600
1,000
800
600
5
400
300
200
100
Number
2% Formaldehyde Agarose
（base）
2% Native Agarose
−80℃
Bromophenol Blue
Orange G
400
300
a
b
c
d
e
f
200
100
Marker 4
(φX173/Hae Ⅲ)
Product PCR
(299 bp) 50 ng
TE Buffer
1 % agarose S
2 % agarose S
3 % agarose 21
Product name
6 × Loading Buffer Triple Dye
6 × Loading Buffer Orange G
6 × Loading Buffer Triple Dye
6 × Loading Buffer Orange G
6 × Loading Buffer Triple Dye
6 × Loading Buffer Orange G
Orange G
Bromophenol Blue Xylene Cyanol FF
100 bp (approx.) 900 bp (approx.) 5,000 bp (approx.)
30 bp (approx.) 300 bp (approx.) 1,500 bp (approx.)
10 bp (approx.) 100 bp (approx.)
500 bp (approx.)
Code No.
Package Size
1
RNA Size Standard Marker Ⅱ（0.5-9 kb）
542-00651
50 μg
2
RNA Size Standard Marker Ⅲ（0.1-1 kb）
545-01621
50 μg
3
RNA Size Standard Marker Ⅳ（0.28-6.58 kb）
188-01831
50 μL
311-06261
25 μL
317-06263
25 μL × 2
4
RNA Ladder（0.125-6 kb）
5
RNA Step Ladder（0.1-1 kb）
298-61301
40 μL × 5
−
Loading Buffer
313-90111
10 mL
−
6 × Loading Buffer OrangeG
317-90251
1 mL × 3
−
6 × Loading Buffer Double Dye
313-90351
1 mL × 3
−
6 × Loading Buffer Triple Dye
314-90261
1 mL × 3
Note
: Nippon Geneʼs products
7
Protein Size Marker
Protein Size Marker
Prestained Marker (1, 2) : With dye combined in its recombinant protein content.
protein G with the ability to combine with the Fc region
Western Marker (3) : Contains
of immunoglobulin. Reacts with both primary and secondary
antibodies in a western blot.
biotinylated protein. Capable of both chromogenetic and
Biotinylated Marker (4) : Contains
photogenetic detection by reacting with avidin HRP or avidin AP.
（kDa）
−20℃
140
（kDa）
240
140
100
100
−20℃
70
50
40
−20℃
60
50
42
35
42
31
21
25
15
14
16
2
Prestained
79
50
15
1
150
120
100
80
25
20
20
（kDa）
70
35
30
（kDa）
7
3
PrestainedⅡ
4
Western
Biotinylated
Marker for CBB Dyeing (5~8)
Optimized for CBB dyeing. Obtains sharp, uniform bands because of a reduction alkyl process.
（kDa）
（kDa）
（kDa）
（kDa）
200
180
116
97
79
116
30
20
17
6.5
3.5
For low molecular
range
79
42
42
30
30
20
14
17
42
42
5
79
30
20
14
6.5
6
7
For middle molecular
range
For high molecular
range
8
Wide range
−20℃
: Store at ｰ20℃
NO DISPLAY: Store at 2〜10℃
Number
Wako Cat. No.
Package Size
1
WIDE-VIEW TM Prestained Protein Size Marker
Product name
230-02221
500 μL
2
WIDE-VIEW TM Prestained Protein Size Marker 2
239-02291
500 μL
3
WIDE-VIEW TM Western Size Marker
233-02211
250 μL
4
WIDE-VIEW TM Biotinylated Size Marker
233-02331
1.5 mL
5
Molecular Weight Marker, Low Range
294-63101
1 mL
6
Molecular Weight Marker, Middle Range
131-14511
1 mL
7
Molecular Weight Marker, High Range
134-14501
1 mL
8
Molecular Weight Marker, Wide Range
296-63301
1 mL
8
Easy SeparatorTM (Wako Catalog No.058-07681 (1 set ) )
Easy SeparatorTM is an electrophoresis tank specially designed for “SuperSep” products to enable easy setting
and removal of the gel plate.
Features
1 Easy to operate.
2 Requires only a small amount (about 250-350ml) of
running buffer for electrophoresis.
3 Allows the gel plate to be removed without removing the
running buffer after electrophoresis has been completed.
Easy Set
Easy Remove
①Insert a gel.
②Turn the knobs
toward you.
③Setting is
completed.
Negative Gel Stain MS Kit
①Remove the cover
②Turn the knobs
after electrophoresis
away from you.
has been completed.
③Remove the gel
without removing
the running buffer.
（Store at room temperature）
Protein bands separated by SDS/polyacrylamide gel electrophoresis (SDS-PAGE) can be visualized as stable,
clear and transparent bands against the background of milky white gel stained by negative gel stain containing
our improved imidazol derivative reagent in as little as 10 minutes. The staining technique is useful to obtain the
clear and sensitive resolution pattern of the gel before immunoblotting as well as to excise and purify the band
of interest from the gel without significant deterioration of amino acid residues for the subsequent studies of
protein such as sequencing and mass analysis of peptide.
Featureas
Procedure
SDS-PAGE gel
1 Detectable limit of protein: 1 ng
Staining Sol. A
2 Sensitive and Simple procedure with 2-step staining in
Shake 5 ~ 10 min.
Wash 5 ~ 10 sec. x 3
5~10 minutes
3 Optimized the staining intensity by either de-staining the
Staining Sol. B
excess stain or re-staining following de-stain of the gel.
Shake 10 ~ 60 sec.
Wash 1 min. x 3
Mass Spectrometry
4 Applicable to proteome analysis by mass spectrometry
and sequencing
Check staining intensity
of the gel against a sheet
of black paper
MALDI-TOF/MS analysis
SDS-PAGE analysis
1
2
3
a
Figure 1.
b
SDS-PAGE analysis of proteins using
Negative Gel Stain MS Kit
c
d
a : rat phosphorylase (97k);
b : bovine serum albumin (68k);
c : hen egg white ovalbumin (45k);
d : bovine carbonic anhydrase (31k).
Lane 1: 50 ng; Lane 2: 100 ng; Lane 3: 150 ng
relative abundance
rat muscle glycogen phosphorylase (EC2.4.1.1)
Figure 2.
MALDI-TOF/MS of
1000 1100 1200 1300 1400 1500 1600 1700 1800 1900 2000 rat phosphorylase
m/z
The band was excised and treated with trypsin. Following the in-gel digestion and
preparation, the sample was analyzed on MALDI-TOF mass spectrometer. (These data
were provided by Dr. Y. Wada at Osaka Medical Center, Japan.)
Product name
Wako Cat. No.
Package Size
293-57701
20 tests
Negative Gel Stain MS Kit
Kit Contents:
1) Staining Solution A (1 × 500 mL); 2) Staining Solution B (1 × 500 mL); 3)De-staining Solution (1 × 500 mL)
9
Electrophoresis-related Products
Electrophoresis-related Products
Electrophoresis-related Products
Electrophoresis-related Products
SuperSepTM Ace
SuperSep™ Ace is a polyacryla
polyacrylamide
amide gel of the highest quality
quality,
designed to resolve the problems of existing products (SuperSep™ and
SuperSep™ HG). Tris-glycine buffer is used in the gel buffer, similar to
existing products, but because its pH is neutralized, the gel’s chemical
storage stability is improved. Physical storage stability is also improved
by using vacuum packaging. And because it does not contain SDS, it
can be used in SDS-PAGE and Native-PAGE by changing the running
buffer.
Features
13 well
17 well
t
enien
Conv er for
stick el
g
n!
catio
i
f
i
t
n
ide
1 Good separation and uniform bands
2 Can be used with a multi-channel pipette.
3 Reasonably priced and capable of long-term storage (for 6 months)
4 Box sticker allows easy identification of type of gel.
5 Comes in 13-well and 17-well types.
100 × 100 × 3（mm）
oved
Impr ation
r
sepa center!
e
h
t
near
90 × 85 × 1（mm）
（kDa）
Product specifications
Plate size
Gel size
13 wells
17 wells
30 μL
25 μL
200
150
3.3 〜 6.5 μg
1.3 〜 3.9 μg
100
75
Number of wells
Well volume
Total amount of protein＊ (/well)
SuperSepTM
SuperSepTM
Ace
＊Indicates total amount of protein that can be clearly separated.
50
rm
Unifo d!
ban
37
① SuperSepTM: 10%
0
1.5
3
4.5
6
7.5
9
10.5
12 (hours)
25
TM
②SuperSep
Ace: 10%
20
（Store at 2〜10℃）
Product name
Wako Cat. No.
Package Size
SuperSepTMAce, 6%,
13well
195-15171
10 ea.
SuperSepTMAce, 6%,
17well
192-15181
10 ea.
SuperSepTMAce, 7.5%,
13well
198-14941
10 ea.
SuperSepTMAce, 7.5%,
17well
191-14931
10 ea.
SuperSepTMAce, 10%,
13well
195-14951
10 ea.
SuperSepTMAce, 10%,
17well
192-14961
10 ea.
SuperSepTMAce, 12.5%,
13well
199-14971
10 ea.
SuperSepTMAce, 12.5%,
17well
196-14981
10 ea.
SuperSepTMAce, 15%,
13well
193-14991
10 ea.
SuperSepTMAce, 15%,
17well
190-15001
10 ea.
SuperSepTMAce, 5-12%, 13well
199-15191
10 ea.
SuperSepTMAce, 5-12%, 17well
192-15201
10 ea.
SuperSepTMAce, 5-20%, 13well
197-15011
10 ea.
SuperSepTMAce, 5-20%, 17well
194-15021
10 ea.
SuperSepTMAce, 10-20%, 13well
191-15031
10 ea.
SuperSepTMAce, 10-20%, 17well
198-15041
10 ea.
SuperSepTMAce, 15-20%, 13well（Tricine Gel）
198-15301
10 ea.
10
SuperSepTM DNA (15%, 17 wells) (Wako Catalog No.190-15481 (10 ea.) )
（Store at 2〜10℃）
Polyacrylamide gel is used in the separation of low-molecular-weight DNA because it has a finer gel mesh
structure than agarose. However, the DNA size will vary widely depending on the acrylamide concentration and
the degree of cross-linkage of the gel. In SuperSepTM DNA, the acrylamide concentration and the degree of
cross-linkage of the gel have been optimized for the separation of low-molecular-weight DNA. This product is
therefore effective for the separation of samples with a DNA size from 20-200 bp.
Features
Product specifications
1 The acrylamide concentration, degree of
Plate size
cross-linkage, and number of wells are optimized for
the electrophoresis of low-molecular-weight DNA.
100 × 100 × 3（mm）
90 × 85 × 1（mm）
Gel size
17 wells
Number of wells
2 Cleaner separation image than that obtained with
25 μL
Well volume
agarose gel.
3 Ideal for 20-200 bp separation.
（bp）
（bp）
100
90
80
70
60
50
40
30
20
（bp）
（bp）
200
100
90
80
70
60
50
150
200
150
100
100
75
75
50
50
25
40
30
25
20
SuperSepTM DNA
（15%, 17 well）
1% Agarose
2.5% Agarose
SuperSepTM DNA
（15%, 17 well）
Example of electrophoresis
20-200 bp DNA was clearly separated by using SuperSepTM DNA and Tris-glycine buffer. PCR products under 200
bp can be used for separation from gel, especially when cloning small RNA. 30-200 bp DNA was clearly
separated with TBE buffer.
① With Tris-Glycine running buffer
（bp）
② With TBE running buffer
（bp）
（bp）
（bp）
200
150
200
150
100
100
50
50
30
25
20
30
20
EtBr staining
25
EtBr staining
Staining Reagent
Product name
Wako Cat. No.
Package Size
EtBr Solution
315-90051
10 mL
11
Selection Guide
【How to read the chart】
1 , p1
●
Number Reference page
【Display codes】
NO DISPLAY Store at 2〜10℃
−80℃ Store at or below ｰ80℃
−20℃ Store at or below ｰ20℃
−70℃ Store at or below ｰ70℃
DNA Ladders
10 bp
1 kbp
100 bp
100 kbp 200 kbp
10 kbp
1 , p2
●
2 , p2
●
3 , p2
●
4 , p2
●
5 , p2
●
6 , p2
●
7 , p2
●
8 , p2
●
9 , p2
●
10 , p2
●
11 , p2
●
12 , p2
●
13 , p3
●
14 , p3
●
15 , p3
●
16 , p3
●
17 , p3
●
18 , p3
●
19 , p4
●
20 , p4
●
21 , p4
●
22 , p4
●
23 , p4
●
24 , p4
●
25 , p4
●
26 , p4
●
27 , p4
●
28 , p4
●
29 , p4
●
30 , p4
●
31 , p5
●
32 , p5
●
33 , p5
●
39 , p6
●
40 , p6
●
41 , p6
●
42 , p6
●
10 bp
1 kbp
100 bp
100 kbp 200 kbp
10 kbp
RNA Ladders
10 b
10000 b
1000 b
100 b
1 , p7
●
2 , p7
●
3 , p7
●
4 , p7
●
5 , p7
●
10 b
10000 b
1000 b
100 b
Protein Size Markers
10 kDa
1000 kDa
100 kDa
1 , p8
●
2 , p8
●
3 , p8
●
4 , p8
●
5 , p8
●
6 , p8
●
7 , p8
●
8 , p8
●
10 kDa
100 kDa
1000 kDa
・This brochure may contain products that cannot be exported to your country due to regulations.
・Listed products are intended for laboratory research use only, and not to be used for drug, food or human use.
co.jp
08X05開01
09Y3IBF