Title
Authorsِ
Detection of Meat Species in Food Using a Single Primer Pair by
PCR and a Novel DNA Extraction by Short Microwave
Irradiation
Abd-El Aziem Farouk, Mohamed Faizal Noor Batcha, Ibrahim
Al-Araidh, and Ralf Greiner
Contact Info
aa_farouk@yahoo.com.
Department
Major
Citation
Science – Teachers College
Science
Food Biotechnology, 22:171–178, 2008
Copyright © Taylor & Francis Group, LLC
2008
Year of
Publication
Publisher
Sponsor
Type of
Publication
ISSN
URI/DOI
Full Text
(Yes, No)
Key words
Abstracts
Taylor &Francis
Paper
0890-5436 print
http://dx.doi.org/10.1080/08905430802043222
DOI: 10.1080/08905430802043222
yes
microwave; meat; detection; PCR; primers
A unique DNA isolation protocol based on microwave irradiation and density
centrifugation
for the simultaneous detection of porcine, chicken, and mutton meat species in
food by PCR using newly developed primers is described. Emphasis is placed on
the
detection of pork, as it is considered unlawful in the diet in major religions such as
Islam and Judaism. The method has been optimized using the primers designed
from
porcine mitochondrial DNA (mtDNA) sequence and tested in 96 samples. The
method
involves 35 seconds of high microwave irradiation of lysed sample homogenate
followed
by a 5- min centrifugation in 1.5 mL Eppendorf tubes until a clear supernatant is
obtained. The design of this protocol makes it possible to process many samples in
a
short time. To evaluate the method pork and food spiked with different
concentrations
of pork together with unknown food samples were analyzed by PCR using the new
set
of porcine specific primers. The new set of primers and the method involved
showed
high sensitivity in detecting porcine and chicken content in food in addition as a
marker for the detection of mutton. The present sample preparation method has the
potential to be applied to other meat detection systems as well.