CURRICULUM VITAE
Personal Information
Name:
Saiqa Andleeb
Discipline:
Biological Sciences
Institution:
University of Azad Jammu and
Kashmir, Muzaffarabad
Gender:
Female
Department:
Zoology
Specialization:
Biotechnology/Genetic
engineering
Present Position: Lecturer
Address:
Department of Zoology,
University of Azad Jammu
City:
and Kashmir,
Muzaffarabad
Muzaffarabad
Email:
drsaiqa@gmail.com
+92-5822-960434
Fax:
Educational History
Year
Degree Field of Study
2010
Ph. D
Biotechnology
2006
M. Phil
Biotechnology
2003
M. Sc
Zoology
Phone:
N.I.C. #
Institution/ University
Quaid-I-Azam university,
Islamabad/NIBGE,
Faisalabad
Quaid-I-Azam university,
Islamabad/NIBGE,
Faisalabad
37405-8873179-6
Title of Thesis
Expression and binding studies of
some Gemini viral proteins
Cloning
and
Expression
of
Chaetomium thermophilum Xylanase
II-A gene in prokaryotic BL21 E.coli
and eukaryotic Pichia pastoris
expression systems
University of Arid
Biology of Parasitoid Aganaspis daci
Agriculture, Rawalpindi (Weld) (Hymenoptera: Eucoilidae)
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Professional Experience
Institution/ Organization
Position / Job Title
Period
University of AJ&K
Lecturer
From
2010
To
Date
National Institute for Biotechnology and
Genetic Engineering (NIBGE)
Researcher
2009
2010
Botanical Institute, Switzerland
Research fellow
2008
2009
National Institute for Biotechnology and
Genetic Engineering (NIBGE)
Researcher
2006
2008
Research Interests
Differential display analysis, protein expression in prokaryotes, yeast and plants, RNA interference,
molecular cloning techniques, antimicrobial activity, Proteomics, Bioinformatics
Projects
“Antimicrobial activity of some traditional herbal drugs against animal and human pathogens” approved
project by University Research Committee (URC) of Azad Jammu and Kashmir University for one year
(2011-2012) of Total Rs. 150,000/- only.
Publications in HEC recognized journals (Total IF- 4.83)
1. Affia Rafique, Saiqa Andleeb*, Tahseen Ghous, Nosheen Shahzad, Irsa Shafique, Atiya Zafar,
Ayesha kiyani. Sensitivity of poultry associated Pseudomonas aeruginosa against Traditional herbs
and standard antibiotics, J Pharm Sci Innov, 1(5), 12-16, (2012).
2. Saiqa Andleeb*, Sara Shamim, Muhammad Naeem Awan, Riaz Aziz Minhas. Modified protocol
for genomic DNA extraction from newly plucked feathers of Lophura leucomelana hamiltoni
(Galliformes) for genetic studies and its endo-restriction analysis. Pak. J. Sci. Ind. Res. 55 (2):108113, (2012).
3. Faiz-ul-Hussan Nasim, Saiqa Andleeb*, Mazhar Iqbal, Tahseen Ghous. Evaluation of antimicrobial
activity of extracts of Fresh and Spoiled Spinacia oleracea against some mammalian pathogens,
(African Journal of Microbiology Research, 6(29): 5847-5851) (2012).
4. Saiqa Andleeb, Imran Amin, Aftab Bashir, Rob W Briddon and Shahid Mansoor. Transient
expression of βC1 protein differentially regulate host genes related to stress response, chloroplast
and mitochondrial functions. (Virology Journal, 7: 373). (2010)
5. Saiqa Andleeb*, Imran Amin, Muhammad Shafiq Shahid, Rob W. Briddon and Shahid Mansoor.
Heterologous expression of βC1 of Chili leaf curl virus in Pichia pastoris. (African Journal of
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Biotechnology 9(47): 8023-8031. (2010)
6. Saiqa Andleeb*, Muhammad Shafiq Shahid and Riaz Mehmood. Biology of Parasitoid Aganaspis
daci (Weld) (Hymenoptera: Eucoilidae). Pak. J. Sci. Ind. Res. 53 (4): 201-204, (2010).
7. Saiqa Wajid*, Shafiq Shahid, Farooq Latif, Zahid Mukhtar, Sher Afzal and Shahid Mansoor.
Heterologous expression of Chaetomium thermophilum Xylanase 11-A (CtX 11-A) gene. Pak. J.
Sci. Ind. Res. 52(2): 100-106 (2009)
8. Muhammad Shafiq Shahid, Liaqat Ali, Saiqa Andleeb. The function of the a-rich region of the
alpha satellite associated with the cotton leaf curl disease in Pakistan. EurAsia. J. BioSci. 3(18), 152156 (2009).
9. Muhammad Shafiq Shahid, Liaqat Ali, Saiqa Wajid. Cotton Leaf Curl Rajasthan Virus infecting
Tomato in Pakistan (Pakistan Journal of Science and Industrial Research) Pak. J. Sci. Ind. Res. 52
(6): 319-321 (2009).
10. Saiqa Andleeb*, Farooq Latif, Sher Afzal, Zahid Mukhtar, Shahid Mansoor and Ibrahim Rajoka.
Cloning and Expression of Chaetomium thermophilum Xylanase II-A gene in Pichia pastoris. The
Nucleus, 45 (1-2): 75-81 (2008).
11. Saiqa Wajid*, Farooq Latif, Sher Afzal, Zahid Mukhtar Abdul Ghaffar, Shahid Mansoor and
Ibrahim Rajoka. Cloning and Expression of Chaetomium thermophilum Xylanase II-A gene in
prokaryotes. The Nucleus, 45 (3-4): 149-156 (2008).
Graduate and Post graduate supervision
Type
Role
M. Phil
Supervisor
No. of Students Supervised & No. of Students In Progress
Awarded Degrees
HEC Funded Non-HEC
Funded
0
NIL
5
M. Sc
Supervisor
12
NIL
12
M. Sc, 2013
Supervisor
0
NIL
5
Ongoing Research projects (2012)
1. Study of antioxidant, antibacterial and phytochemical activity of Mint (Mentha spicata) and Basil
(Ocimum basilicum L.) on variety of human and fish associated pathogenic bacteria
2. Comparative studies of the antibacterial effects of garlic (Allium sativum Linn) ginger (Zingiber
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officinale Roscoe) and honey against some Human and fish associated pathogens
3. Evaluation of in vitro antibacterial and antioxidant activities of extracts of Orange peel against
human and fish associated bacterial pathogens
4. Antibacterial activity of various solvent extracts and excreta of Eisenia fetida against human
bacterial pathogens
Student supervised M. Sc and M. Phil
Student
Uzma Azeem Awan
Nazia Riaz
Basharat Mehmood
Madiha Khalid
Maimoona Tahir
Kamran Khurshid
Dar
Mubashir Ejaz
Dissertation
M. Phil
M. Phil
M. Sc
M. Sc
M. Sc
Title
M. Phil thesis titles
Comparative studies of the antibacterial effects of
garlic (Allium sativum Linn) ginger (Zingiber
officinale Roscoe) and honey against Human
associated pathogens
Study
of
antioxidant,
antibacterial
and
phytochemical activity of Mint (Mentha spicata)
and Basil (Ocimum basilicum L.) on variety of
human pathogenic bacteria
M. Sc thesis titles
Evaluation of in vitro antibacterial and antioxidant
activities of solvent extracts of Orange peel against
human bacterial pathogens
Screening of antibacterial and antioxidant activity
of extracts of basil and mint against fish infectious
pathogens
In vitro screening of garlic (Allium sativum Linn)
ginger (Zingiber officinale Roscoe) against
bacterial pathogens associated with fish products
Year of graduation
Thesis write up
Thesis write up
Ongoing
Ongoing
Ongoing
M. Sc
In vitro evaluation of antibacterial and antioxidant
activities of extracts of Orange peel against fish
associated bacterial pathogens
Ongoing
M. Sc
Antibacterial activity of various solvent extracts
and excreta of Eisenia fetida against bacterial
Ongoing
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pathogens
Affia Rafique
Amna Shahnawaz
Summya Ghous
Sundus Siddique
M. Sc
Analysis of sensitivity of poultry associated
Pseudomonas aeruginosa against medicinal plants
and standard antibiotics
2012
M. Sc
Isolation and identification of Eisenia fetida
associated Pseudomonas aeruginosa spp. and its
control
2012
M. Sc
Assessment of extracts of Momordica charantia,
antibiotics and organic compounds against water
associated
Enterococcus
faecalis
and
Enterococcus faecium
2012
M. Sc
In vitro screening of medicinal herbal,
organometallic compounds extracts and antibiotics
against fish products at retail outlets
2012
Screening of antibacterial activity of medicinal
plants, organometallic compounds and antibiotics
against spoiled fish pathogens
2012
Identification and Investigation of Antibacterial
activity of antibiotics, organic compounds and
medicinal plants against Human associated
bacterial pathogens
2012
Evaluation of antibacterial activity of several
extracts of organometallic compounds, antibiotics
and medicinal plants against Human pathogenic
microbes
2012
Identification of unknown bacterial pathogens
through various Biochemical techniques
2012
M. Sc
The genomic DNA and protein extraction from the
fresh feathers of Lophura leucomelanos and its
endo-restriction analysis
2011
M. Sc
Effect of selected antibiotics against different
microbes through standard diffusion disc method
and isolation of genomic DNA through modified
methods.
2011
M. Sc
Ume-Kalsoom
M. Sc
Aysha Kiyani
M. Sc
Atia Zafar
Irsa Shafique
Sara Shamim
Asma Parveen
M. Sc
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Farhat Yasmeen
Mariam Jamil
M. Sc
Optimization of genomic DNA and protein
isolation from molted feathers of Himalayan
Griffon Vulture through modified methods.
2011
M. Sc
Antibacterial activity of some antibiotics against
Pseudomonas syringae and Staphylococcus aureus
and genomic analysis through standard diffusion
disc method and bioinformatics tools.
2011
Workshops and trainings
1. International Workshop on Marine Biology: Ecology and Applications, Advancements and
Issues of the 21st Century. 26-28 June 2012, COMSTECH Building, 33-Constitution Avenue, G5/2, Islamabad, Pakistan
2. Two day workshop on Capacity Building in Geospatial Information and Environmental Data,
4-5 July 2011, Muzzafarabad, Azad Jammu and Kashmir.
3. One day work shop on Propagation of QEC concepts and SAR writing methods, organized by
QEC at University of AJ&K on 19-03-2010.
4. Six months training program under scheme “International Research Initiative Support program”
IRISP, HEC scholarship from June 2008-December 2008.
5. ICGEB-Egypt Workshop on “Cloning and gene expression in prokaryotes” (Training course in
Genetic Engineering and Biotechnology Research Institute (GEBRI) Mubarak City For Scientific
Research and Technological Applications (MCSRTA),Research Area, New Borg El-Arab City,Post
Code 21934, Alexandria, Egypt.)
6. Workshop on fiber degrading enzymes: Impact on poultry feed and prospects of
commercialization. October 4, 2005.
Conferences/Seminars
1. Saiqa Andleeb*.Cloning and Expression of AV2 Tomato leaf curl New Delhi virus (ToLCNDV)
protein through Bacterial and Gateway recombination systems. ‘Second National Conference on
Biotechnology and Microbiology, 2012, July 7-10, 2012
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2. Saiqa Andleeb*, Tahseen Ghous, Nosheen Shahzad, Afia Rafique. Analysis of sensitivity of
poultry associated Pseudomonas aeruginosa against medicinal plants and standard antibiotics. 32nd
Pakistan congress of Zoology (International), March 6-8, 2012. CBGP-17, pp, 11.
3. Uzma Azeem, Aysha Kiyani, Atia Zafar, Irsa Shafique, Saiqa Andleeb*. Evaluation of antibacterial
activity of antibiotics and medicinal herbs against some human bacterial pathogens. 32nd Pakistan
congress of Zoology (International), March 6-8, 2012. CBGP-19, pp, 12
4. Sobia Nazir, Uzma Azeem, Saiqa Andleeb*, Nazia Riaz, Sadiq-ur-Rehman. Insecticidal and
antibacterial investigations of organometallic compounds against pathogens. 32nd Pakistan congress
of Zoology (International), March 6-8, 2012. CBGP-20, pp, 13.
5. Irsa Shafique, Aysha Kiyani, Atia Zafar and Saiqa andleeb*. Identification of unknown bacterial
pathogens through various biochemical techniques. 32nd Pakistan congress of Zoology
(International), March 6-8, 2012. CBGP-13, pp, 8-9.
6. Tahseen Ghous, Saiqa Andleeb*, Nosheen Shahzad, Ume Kalsoom, Sundus Siddique. Screening of
antibacterial activity of medicinal plants and antibiotics against fish products at retail outlets. 32 nd
Pakistan congress of Zoology (International), March 6-8, 2012. CBGP-138, pp, 80.
7. Nazia Riaz, Nosheen Shahzad, Summya Ghous, Saiqa Andleeb*, and Tahseen Ghous. Assessments
of extracts of Momordica charantia and antibiotics against water associated Enterococcus faecalis
and Enterococcus faecium. 32nd Pakistan congress of Zoology (International), March 6-8, 2012.
CBGP-139, pp, 80-81.
8.
Amna Shahnawaz1, Nosheen Shahzad2, Tehseen Ghous2, Saiqa Andleeb1*. Isolation and
identification of Eisenia fetida associated Pseudomonas aeruginosa spp. and its control. 32nd
Pakistan congress of Zoology (International), March 6-8, 2012. Poster 17, pp, 329.
9. Farhat Yasmeen, Riaz Aziz Minhas and Muhammad Saddique Awan, Saiqa Andleeb*.
Optimization of genomic DNA and protein isolation from molted feathers of Himalayan Griffon
Vulture through modified methods. 31st Pakistan congress of Zoology (International), April 19-21,
2011. CBGP-19, pp, 12.
10. Asma Parveen and Saiqa Andleeb*. Effect of selected antibiotics against different microbes
through standard diffusion disc method and isolation of genomic DNA through modified methods.
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31st Pakistan congress of Zoology (International), April 19-21, 2011. CBGP-18, pp, 11
11. Sara Shamim, Riaz Aziz Minhas and Muhammad Saddique Awan, Saiqa Andleeb*.The genomic
DNA and protein extraction from the fresh feathers of Lophura leucomelanos and its endorestriction analysis. 31st Pakistan congress of Zoology (International), April 19-21, 2011. CBGP-17,
pp, 11.
12. Mariam Jamil, Saiqa Andleeb*. Antibacterial activity of some antibiotics against Pseudomonas
syringae and Staphylococcus aureus and genomic analysis through standard diffusion disc method
and bioinformatics tools. 31st Pakistan congress of Zoology (International), April 19-21, 2011.
CBGP-20, pp, 13.
13. International conference on Advance in Agriculture: prospects and potentials of natural resources in
food security.11-13 August.2009
14. First international conference on the role of chemistry and biochemistry in the national
development. Department of chemistry and institute of biochemistry, University of Balochistan,
Quetta, Pakistan. 16-18 April, 2007
15. Characterization and Management of emerging viral diseases in the developing world. Ist
International Training course/Symposium 14-22 Nov.2006, Pakistan, Faisalabad
16. First International Science Conference in Azad Jammu and Kashmir University. Muzaffarabad, 1921, Sep, 2005.
Professional Contributions
Reviewer of „‟Virus Gene‟‟ Journal
Reviewer of “African journal of Biotechnology”
Reviewer of “Yeast” Journal
Reviewer of “African journal of Microbiological research”
Computer Skills
1. Efficient use of NCBI data base, ExPASY, justbio.com, Kobas analysis.
2. Skills in using the programs DNA star (Edit sequence, Seq man, Gene quest, Map draw, Meg Align,
Primer Select.). Clustal X and Tree view.
3. Proficient in MS- Word, Power Point, and MS- Excel
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4. pDraw32 1.1.101 ACOCLONE software (Map designing and orientation, used for restriction
analysis, open reading frames finding)
References
1. Dr. Shahid Mansoor, (Sitara-e-Imtiaz)
Principal Scientist (PS) & Supervisor
Molecular Virology and Gene Silencing lab,
Head Agricultural Biotechnology Division.
NIBGE P.O. Box- 577, Jhang Road,
Faisalabad, Pakistan.
E-mail: shahidmansoor7@gmail.com
2. Dr. Hesham Elsayed Mostafa
Genetic Engineering and Biotechnology Research Institute (GEBRI)
Mubarak City For Scientific Research and Technological Applications (MCSRTA),
Research Area, New Borg El-Arab City,
Post Code 21934, Alexandria, Egypt.
E.mail: hesham1968@yahoo.com
3. Prof. Dr Thomas Hohn
Foreign Supervisor,
Molecular Virology and Gene Silencing lab,
University of Basel, Botanical Institute.
Basel, Switzerland.
E-mail: Thomas.hohn@fmi.ch
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