Iron oxide nanoparticles with potential applications in the purification of
nucleic acids
José R. Sosa-Acostaa, Leunam Fernández-Izquierdob, Judith CastilloRodríguezb, Linnavel Jiménez-Hernándezc, Alicia M. Díaz-Garcíaa
a
Department of Inorganic Chemistry - Bioinorganic Laboratory, Faculty of Chemistry, University
of Havana, Cuba
b Center
c
for Advanced Studies of Cuba, Ministry of Science Technology and Environment
Institute of Material Sciences and Technology (IMRE), University of Havana, Cuba
jrsosa@fq.uh.cu
Nucleic acids purification is considered a critical step in detecting both viral and
genomic diseases. The quality of the purification will determine the sensitivity and
specificity of the diagnostic assay. Appropriate modification of magnetic nanoparticles
(MNP) with stabilizers that contain certain groups capable of forming hydrogen bond,
allow a quickly and easily nucleic acids purification. [1] This research is intended to
obtain MNP by coprecipitation method followed by the modification with hydroxyl
groups as stabilizers and silicate and 3-aminopropyl trietoxysilane. [2] The unmodified
and modified MNP were characterized by FT-IR spectroscopy, XRD and SEM. A
characteristic band, around 575 cm-1, assignable to Fe-O valence vibration of
magnetite was observed in the IR spectrum. From XRD pattern analysis, it appears
that the obtained materials correspond to magnetite phase (JCPDS 19-629). Spherical
morphology of MNP was observed by SEM. Preliminary extraction tests of oligomer
were studied by UV-Vis spectroscopy at 260 nm.
[1] Z. M. Saiyed; J. Phys. Condens. Matter, 2008,20(20), 204153.
[2].W. U.Neng-Biao; Chinese Journal of Biochemistry and Molecular Biology, 2009,
25(10), 958-962.