THESIS INFORMATION Thesis title: Specialization: Study on the biological characteristics, culture and use of freshwater rotifer (Brachionus angularis) Freshwater Aquaculture Training field Code: 62 62 60 01 Student: Tran Suong Ngoc Supervisors: A/Prof. Dr. Vu Ngoc Ut, Can Tho University A/Prof. Dr. Truong Quoc Phu, Can Tho University Training institution: Can Tho University I. THESIS SUMMARY Study on the biological and reproductive characteristics of freshwater rotifer Brachionus angularis with the aim to evaluate its potential in different natural habitats. And based on these characteristics a culture protocol (procedure) has been made for freshwater rotifer Brachionus angularis, which will contribute to the hatchery phases of target fresh- or brackish species recently. Thesis has covered (1) distribution of fresh water rotifer B angularis in different eco-systems (e.g. run-off, static, fresh-water, brackish water and acidic waters), (2) biological characteristics included of embryo development, maturation, fecundity, brood-interval, life-span…in correspondent temperature, pH and salinity; (3) feeding strategy (Chlorella and yeast), initial stocking density (200, 300, 400 and 500 ind./mL), water exchange (10%, 20% và 30%/day), biomass collecting rate (15%, 25% và 35%/day), culture parameters (temperature, pH and salinity) on the fresh water rotifer B. angularis with the aim to develop a culture procedure for optimal biomass production; and (4) application of freshwater rotifer B. angularis in nursery of Sand goby or Marbled sleeper (Oxyeleotris marmoratus Bleeker) (optimal feeding level and culture procedure with and without green-water system) in order to improve their survival during day 1-10. II. NEW FINDING OF THE THESIS The freshwater rotifer Brachionus angularis (isolated in the Mekong Delta, South of Vietnam) could distribute at very low pH (≤4) and brackish water (up to 5 ppt) in the estuaries, this except unique character could not find in the other strain of B. Angularis in the world, and thus they are suitable in mass culture for initial feeding to a number of fresh- and/or brackish water species in the Mekong Delta. Compared to the other strains, B. Angularis on this study shows its tiny size and quick reproduction, high fecundity and fast population development, which are feasible in biomass production for feeding to early stages of fries, especially those characterized with too small mouth dimension. Application of B. Angularis in nursery of Sand goby (or Marble slepper) Oxyeleotris marmoratus Bleeker during day 0-10 helped to improved considerably their survival compared to the traditional feed (yolk and soya flour) from 19,3 % to 43,6%, respectively. This proved significantly that feeding strategy has to meet not only nutritional level but appropriate feed particle is crucial. Moreover, acclimatization of B. Angularis up to 5 ppt 1 (or even higher) to bring up a possibility of mass culture of this species to fulfill the live feed requirement of valuable species in aquaculture. III. APPLIED FINDINGS AND FUTHER RESEARCH NEEDS 3.1. Applicable possibility of thesis’s out-come Finding and completion of basic knowledge on biological and reproductive characteristics of freshwater rotifer B. angularis for lecture note and further investigation for production of this valuable live-feed. Culture and application of live-feed B. angularis in the hatcheries of target species (Giant gourami, Discus fish) will improve their survival and cost effective in production of these species in aquaculture. 3.2. Further researches necessary - Acclimatization to salinity higher than 5 ppt to fill-up a high demand of (small) rotifer in marine species hatcheries is expected. - Scaling-up and modified culture protocol for appropriate culture volume may needed - Application of B. Angularis to other valuable freshwater species should be considered. Supervisors A/Prof. Dr. Vu Ngoc Ut Student A/Prof. Dr. Truong Quoc Phu CERTIFIED BY TRAINING INSTITUTION RECTOR 2 Tran Suong Ngoc 3