An Introduction to Genetic Analysis
Preface
1. Genetics and the Organism
Introduction
Genes as determinants of the inherent
properties of species
Genetic variation
Methodologies used in genetics
Genes, the environment, and the organism
Summary
Concept Map
Problems
Gene interaction and modified dihybrid
ratios
Gene interaction in petal color of foxgloves
Gene interaction in coat color of mammals
Penetrance and expressivity
Chi-square test
Summary
Concept Map
Chapter Integration Problem
Solved Problems
Problems
5. Basic Eukaryotic Chromosome
2 . Patterns of Inheritance
Introduction
Mendel's experiments
Using genetic ratios
Sex
chromosomes
and
inheritance
Human genetics
Summary
Concept Map
Chapter Integration Problem
Solved Problems
Problems
Mapping
sex-linked
3. Chromosomal Basis of Heredity
Introduction
Historical development of the chromosome
theory
Mendelian genetics in eukaryotic life cycles
Topography of the chromosome set
Three-dimensional
structure
of
chromosomes
Sequence organization
Summary
Concept Map
Chapter Integration Problem
Solved Problems
Problems
4. Gene Interaction
Introduction
From genes to phenotypes
A diagnostic test for alleles
Interactions between the alleles of one gene
Introduction
The discovery of linkage
Recombination
Linkage symbolism
Linkage of genes on the X chromosome
Linkage maps
Three-point testcross
Interference
Calculating recombinant frequencies from
selfed dihybrids
Examples of linkage maps
Chi-square test for linkage
Mapping with molecular markers
Linkage mapping by recombination in
humans
Nature of crossing-over
Summary
Concept Map
Chapter Integration Problem
Solved Problems
Problems
6.
Specialized
Eukaryotic
Chromosome Mapping Techniques
Introduction
Accurate calculation of large map distances
Analysis of single meioses
Mapping genes by mitotic segregation and
recombination
Mapping by in situ hybridization
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An Introduction to Genetic Analysis
Mapping
human
genes
by
human–rodent somatic cell hybrids
Summary
Concept Map
Chapter Integration Problem
Solved Problems
Problems
using
7. Gene Transfer in Bacteria and Their
Summary
Concept Map
Chapter Integration Problem
Solved Problems
Problems
10 . Molecular Biology of Gene
Function
Introduction
Working with microorganisms
Bacterial conjugation
Bacterial transformation
Bacteriophage genetics
Transduction
Chromosome mapping
Bacterial gene transfer in review
Summary
Concept Map
Chapter Integration Problem
Solved Problems
Problems
Introduction
Properties of RNA
Transcription
Transcription and RNA polymerase
Eukaryotic RNA
Translation
Genetic code
Protein synthesis
Universality of genetic information transfer
Functional division of labor in the gene set
Summary
Concept Map
Chapter Integration Problem
Solved Problems
Problems
8. The Structure and Replication of
11. Regulation of Gene Transcription
Viruses
DNA
Introduction
DNA: The genetic material
Structure of DNA
Replication of DNA
Mechanism of DNA replication
Summary
Concept Map
Chapter Integration Problem
Solved Problems
Problems
9. Genetics of DNA Function
Introduction
How genes work
Gene-protein relations
Genetic fine structure
Mutational sites
Complementation
Introduction
Basic control circuits
Discovery of the lac system: negative
control
Catabolite repression of the lac operon:
positive control
Positive and negative control
Dual positive and negative control: the
arabinose operon
Metabolic pathways
Additional examples of control: attenuation
Lambda phage: a complex of operons
Transcription: an overview of gene
regulation in eukaryotes
Regulation of transcription factors
Epigenetic inheritance
Summary
Concept Map
Chapter Integration Problem
Solved Problems
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An Introduction to Genetic Analysis
Problems
12. Recombinant DNA Technology
Introduction
Making recombinant DNA
Cloning a specific gene
Using cloned DNA
Summary
Concept Map
Chapter Integration Problem
Solved Problem
Problems
13. Applications of Recombinant
Somatic versus germinal mutation
Mutant types
Occurrence of mutations
Selective systems
Mutation induction
Mutation and cancer
Mutagens in genetic dissection
Mutation breeding
Summary
Concept Map
Chapter Integration Problem
Solved Problems
Problems
16. Mechanisms of Gene Mutation
DNA Technology
Introduction
In vitro mutagenesis
RFLP mapping
Reverse genetics
Expressing eukaryotic genes in bacteria
Recombinant DNA technology in eukaryotes
Gene therapy
Using recombinant DNA to detect disease
alleles directly
Summary
Concept Map
Chapter Integration Problem
Solved Problems
Problems
Introduction
Molecular basis of gene mutations
Spontaneous mutations
Induced mutations
Reversion analysis
Relation between mutagens and carcinogens
Biological repair mechanisms
Repair defects and human diseases
Summary
Concept Map
Chapter Integration Problem
Solved Problem
Problems
17. Chromosome Mutation I: Changes
14. Genomics
in Chromosome Structure
Introduction
Genomics: an overview
Genome projects: practical considerations
Structural genomics
Functional genomics
Summary
Concept Map
Chapter Integration Problem
Solved Problems
Problems
Introduction
Origin of changes in chromosome structure
Deletions
Duplications
Inversions
Translocations
Diagnosis of rearrangements by tetrad
analysis
Summary
Concept Map
Chapter Integration Problem
Solved Problems
Problems
15. Gene Mutation
Introduction
How DNA changes affect phenotype
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An Introduction to Genetic Analysis
18.
Chromosome
Mutation
II:
21. Extranuclear Genes
Introduction
Aberrant euploidy
Aneuploidy
Mechanisms of gene imbalance
Chromosome mechanics in plant breeding
Summary
Concept Map
Chapter Integration Problem
Solved Problems
Problems
Introduction
Origin of extranuclear genes
Structure of organelle chromosomes
Organelle mutations
Inheritance of organelle genes and mutations
Recombination of extranuclear DNA
Cytoplasmic male sterility
Mitochondria and aging
Summary
Chapter Integration Problem
Solved Problems
Problems
19. Mechanisms of Recombination
22. Cancer as a Genetic Disease
Introduction
Breakage and reunion of DNA molecules
Chiasmata: the crossover points
Genetic results leading to recombination
models
Holliday model
Enzymatic mechanism of recombination
Summary
Concept Map
Chapter Integration Problem
Solved Problems
Problems
Introduction
Cancer and the control of cell number: an
overview
Cell proliferation machinery
Machinery for programmed cell death
Controlling the cell-proliferation and death
machinery
Cancer: the genetics of aberrant cell control
Cancer research in the genomic analysis era
Summary
Concept Map
Chapter Integration Problem
Solved Problems
Problems
Changes in Chromosome Number
20. Transposable Genetic Elements
Introduction
Controlling elements in maize
Bacterial insertion sequences
Prokaryotic transposons
Mechanism of transposition in prokaryotes
Review of transposable elements in
prokaryotes
Molecular nature of transposable elements
ineukaryotes
Review of transposable elements in
eukaryotes
Summary
Concept Map
Chapter Integration Problem
Solved Problem
Problems
23. Developmental Genetics
Introduction
Central themes of developmental genetics
Gene regulation at levels other than
transcription initiation: examples
Binary fate decisions: pathways of sex
determination
Drosophila sex determination: every cell for
itself
Sex determination in mammals: coordinated
control by the endocrine system
Binary fate decisions: the germ line versus
the soma
Forming complex pattern: establishing
positional information
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An Introduction to Genetic Analysis
Forming
complex
pattern:
utilizing
positional information to establish cell fates
Additional aspects of pattern formation
The many parallels in vertebrate and insect
pattern formation
Do the lessons of animal development apply
to plants?
Summary
Concept Map
Chapter Integration Problem
Solved Problems
Problems
24. Population Genetics
Introduction
Variation and its modulation
Effect of sexual reproduction on variation
Sources of variation
Selection
Balanced polymorphism
Artificial selection
Random events
Summary
Concept Map
Chapter Integration Problem
Solved Problems
Problems
26. Evolutionary Genetics
Introduction
A synthesis of forces: variation and
divergence of populations
Multiple adaptive peaks
Heritability of variation
Observed variation within and between
populations
Process of speciation
Origin of new genes
Rate of molecular evolution
Summary
Concept Map
Chapter Integration Problem
Solved Problems
Problems
Genetic nomenclature
Genetic nomenclature
25. Quantitative Genetics
Introduction
Some basic statistical notions
Genotypes and phenotypic distribution
Norm of reaction and phenotypic
distribution
Determining norms of reaction
Heritability of a trait
Quantifying heritability
Locating the genes
More on analyzing variance
Summary
Concept Map
Statistical Appendix
Chapter Integration Problem
Solved Problems
Problems
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