On-line 2D HPLC Coupled with Data Independent Tandem Mass
Spectrometry for Enhanced Quantitative and Qualitative Analysis of
Complex Proteomes
Yishai Levin
Proteomics & Mass Spectrometry Teaching Unit,
Weizmann Institute of Science, Rehovot, Israel
yishai.levin@weizmann.ac.il
Modern proteomics core facilities must live up to the high expectations of their scientific
collaborators and funding agencies. Secure protein identification, high proteome coverage and
confident quantification of expression are expected. Recent innovations in ultra/high performance
liquid chromatography (HPLC), ion mobility separation (IMS), mass spectrometry (MS) and
bioinformatics, that additively increase peak capacity, make these goals achievable in the majority
of cases. We will summarize the analytical characteristics of a novel 2D HPLC-IMS/MS platform for
qualitative and quantitative profiling of complex proteomes.
The addition of electron transfer dissociation (ETD) to our analytical platform has enabled the
reliable identification and mapping of labile post-translational modifications. Examples of the utility
of ETD, in our hands, will be discussed.
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